Raffinose family oligosaccharides in seeds of common vetch (Vicia sativa L. ssp. sativa)

Lahuta, Leśław B.; Rybiński, W.; Bocianowski, Jan; Nowosad, Kamila; Börner, Andreas

doi:10.18805/lr-491

Cited by 2 publications

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“…Biochemical characteristics of the seeds, for example, the composition and content of soluble carbohydrates (mainly raffinose family oligosaccharides, RFOs), are also commonly used for the estimation of intra- and inter-specific diversity. The suitability of that approach was demonstrated for the species representing the genus Lupinus (Piotrowicz-Cieslak 2005 ), Vicia (Lahuta et al 2018 ; Lahuta et al 2020 ), Lathyrus (Ibrahim et al 2021 ), Agave , Dasylirion (Mancilla-Margalli and Lopez 2006 ), and for Vitis vinifera L. varieties (Bhouri et al 2016 ) or willow cultivars (Budny et al 2021 ). Oat grains are also abundant in sugars and oligosaccharides (MacLeod and McCorquodale 1958 ; Kaur et al 2019 ).…”

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confidence: 99%

The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds

et al. 2023

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Here, we compared the polymorphism among 13 Avena species revealed by the iPBS markers and soluble carbohydrate profiles in seeds. The application of seven iPBS markers generated 83 bands, out of which 20.5% were polymorphic. No species-specific bands were scored. Shannon’s information index (I) and expected heterozygosity (He) revealed low genetic diversity, with the highest values observed for A. nuda (I = 0.099; He = 0.068). UPGMA clustering of studied Avena accessions and PCoA results showed that the polyploidy level is the main grouping criterion. High-resolution gas chromatography revealed that the studied Avena accessions share the same composition of soluble carbohydrates, but significant differences in the content of total (5.30–22.38 mg g−1 of dry weight) and particular sugars among studied samples were observed. Sucrose appeared as the most abundant sugar (mean 61.52% of total soluble carbohydrates), followed by raffinose family oligosaccharides (31.23%), myo-inositol and its galactosides (6.16%), and monosaccharides (1.09%). The pattern of interspecific variation in soluble carbohydrates, showed by PCA, was convergent to that revealed by iPBS markers. Thus, both methods appeared as a source of valuable data useful in the characterization of Avena resources or in the discussion on the evolution of this genus.

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Section: Introductionmentioning

confidence: 99%

The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds

et al. 2023

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“…Common vetch (Vicia sativa subsp. sativa L.) is the most widely grown subspecies used as a winter cover crop or green manure, although it has also been utilized for animal grazing, silage and hay (Seymour et al 2002;Lahuta et al 2020). The subsp.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic Diversity and Characterization of Vicia sativa Subspecies

Benlioğlu

2020

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Background: Vicia sativa L. is variable genus comprised of several subspecies. Close relative species and subspecies of the cultivated species are easily usable gene sources because they have gained resistance against a wide range of biotic and abiotic stresses. The main objectives of this study are to identify and describe the cytogenetical and karyological characteristics of subspecies in the Vicia sativa complex.Methods: The research material consisted of multiple entries collected from the five subspecies of nine taxa. All cytological observations made from root tips. Six chromosomal parameters (chromosome length, relative length, long arm length, short arm length, arm ratio and centromeric index) and five karyotype asymmetric parameters (difference in relative length, total form percentage, intrachromosomal asymmetry index, interchromosomal asymmetry index and mean centromeric asymmetry) were determined.Result: It was determined that the chromosome number of subspecies were 2n =10-12. The haploid chromosome lengths of subspecies were 15.86-33.88 µm and the average chromosome lengths varied between 2.64-5.65 µm. According to the intrachromosomal karyotype asymmetry index analysis, subsp. segetalis was the most asymmetric karyotype and subsp. sativa “Antalya” was the most symmetric karyotype. According to the interchromosomal karyotype asymmetry index analysis, subsp. angustifolia was the most asymmetric karyotype and subsp. nigra was the most symmetric karyotype.

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Raffinose family oligosaccharides in seeds of common vetch (Vicia sativa L. ssp. sativa)

Cited by 2 publications

References 20 publications

The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds

The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds

Cytogenetic Diversity and Characterization of Vicia sativa Subspecies

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