A hydrogen rail (hydrail) powertrain is conceptualized in this study, using drive cycles collected from the trains currently working on the Union Pearson Express (UPE) railroad. The powertrain consists of three preliminary different subsystems: fuel cell, battery, and hydrogen storage systems. A backward design approach is proposed to calculate the time-variable power demand based on a "route simulation data" method. The powertrain components are then conceptually sized according to the calculated duty cycle. The results of this study show that 275 kg of hydrogen is sufficient to satisfy the daily power and energy demand of a hydrogen locomotive with drive cycles similar to the ones currently working on the UPE rail route.World Electric Vehicle Journal 2019, 10, 32 2 of 14 specifically for transportation applications. This can also prevent grid fluctuations and improve the flexibility of the electricity grid and help in controlling the intermittency of renewable systems [7][8][9][10][11][12].Hydrogen transportation is a multidimensional issue that can be analyzed from different aspects including, but not limited to, hydrogen production, refueling station infrastructure, powertrain components topology, sizing, and control. Evaluating the infrastructural requirements and energy consumption of such a system is an essential part towards commercialization. In that sense, the high portion of clean electricity, generated by nuclear and renewable sources, in Ontario's supply mix provides a unique opportunity for development of a hydrogen transportation system. Figure 1, shows Ontario electricity supply mix in 2017 [13].World Electric Vehicle Journal 2019, 10, x FOR PEER REVIEW 2 of 14 fluctuations and improve the flexibility of the electricity grid and help in controlling the intermittency of renewable systems [7][8][9][10][11][12].Hydrogen transportation is a multidimensional issue that can be analyzed from different aspects including, but not limited to, hydrogen production, refueling station infrastructure, powertrain components topology, sizing, and control. Evaluating the infrastructural requirements and energy consumption of such a system is an essential part towards commercialization. In that sense, the high portion of clean electricity, generated by nuclear and renewable sources, in Ontario's supply mix provides a unique opportunity for development of a hydrogen transportation system. Figure 1, shows Ontario electricity supply mix in 2017 [13].