Raised plasma concentration and ex vivo production of inflammatory chemokines in patients with systemic lupus erythematosus

Abstract

Background: Chemokines are involved in leucocyte chemotaxis. Infiltrating leucocytes play an important role of tissue injury in systemic lupus erythematosus (SLE). Objective: To investigate the role of inflammatory chemokines and their association with interleukin 18 (IL18) in SLE pathogenesis and disease activity. Methods: Plasma concentrations and ex vivo peripheral blood mononuclear cell production of inflammatory chemokines IP-10, RANTES, MIG, MCP-1, TARC, IL8, and GROa, and proinflammatory cytokines IL18, IFNc, IL2, IL4, and IL10 were assayed in 80 SLE patients with or without renal disease and 40 healthy controls by immunofluorescence flow cytometry and enzyme linked immunosorbent assay. Results: Plasma IP10, RANTES, MIG, MCP-1, GROa, and IL18 concentrations in all SLE patients were higher than in controls, and correlated significantly with SLEDAI score (all p,0.05). In SLE patients without renal disease, IP10, RANTES, MIG, MCP-1, IL8, and IL18 correlated positively with SLEDAI score, while in those with renal derangement, IP10, IL8, IL10, and IL18 correlated with disease activity (all p,0.05). Plasma IL18 concentration correlated positively with IP10, MIG, GROa, and IL8 in all SLE patients (all p,0.005). Mitogen induced increases in ex vivo production of IP10, MCP-1, TARC, IFNc, IL4, and IL10 were higher in all SLE patients regardless of their difference in disease activity (all p,0.05). Patients with renal disease had an augmented ex vivo release of RANTES. Conclusions: The correlation of raised plasma concentration and ex vivo production of inflammatory chemokines with disease activity, and their association with IL18, supports the view that chemotaxis of Th1/Th2 lymphocytes and neutrophils is important in SLE pathogenesis.

Discrimination between Active and Latent Tuberculosis Based on Ratio of Antigen-Specific to Mitogen-Induced IP-10 Production

“…Therefore, the use of the IP-10 ratio might support an improved diagnosis of active TB disease when combined with the standard diagnostic methods, including microbiology, molecular tests, and clinical and radiological assessments. IP-10 (also known as CXCL10 or IFN-␥-induced protein 10) is a chemokine that is expressed at a high level in inflammatory diseases such as systemic lupus erythematosus, thyroid disease, acute coronary syndrome, and allergies (28)(29)(30). IP-10 is expressed in the bronchial epithelium (31) and in inflamed tissues (32) of active TB patients, who demonstrate upregulation of the helper T (Th) 1-type cytokine IFN-␥.…”

Enhanced expression of interferon-inducible protein-10 correlates with disease activity and clinical manifestations in systemic lupus erythematosus

“…Recently, elevated plasma or serum IP-10 levels were found in several studies of small numbers of SLE patients [14][15][16][17][18]; however, the correlation with disease activity was variable. Thus we sought to explore further the serum levels of this chemokine in SLE and their relationship with disease activity and organ manifestations in a larger cohort of 464 oriental lupus patients.…”

Proteomic analysis of human plasma and peripheral blood mononuclear cells in Systemic Lupus Erythematosus patients

“…Another study showed that plasma concentrations of IP10 and MCP-1 were increased in SLE patients. They also showed a strong positive correlation between IP-10 levels and SLE disease activity and suggested a possible correlation between SLE renal involvement and IP-10 [113].…”

“…In SLE, several of these cytokines are overexpressed and contribute to the pathogenesis, in the activity or organ involvement of the disease. Even chemokines (chemoattractan cytokines) that exert their effects by attracting inflammatory cells, including monocytes, neutrophils and T lymphocytes, to sites of inflammation, are implicated in the pathogenesis of SLE, and in some studies, the expression was found to be increased and correlated with renal involvement and disese activity [45,46].…”