Raloxifene promotes adipocyte differentiation of 3T3-L1 cells

Murase, Yuko; Kobayashi, Junji; Nohara, Atsushi; Asano, Akimichi; Yamaaki, Naoto; Suzuki, Kaoru; Sato, Hiroshi; Mabuchi, Hiroshi

doi:10.1016/j.ejphar.2006.03.033

Cited by 23 publications

(18 citation statements)

References 14 publications

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“…Interestingly, concomitant application of Y-27632 reversed this action, implicating that Rho-kinase may mediate the production /release of resistin by estrogen in any way. Estrogens and the activators of Rho/ROCK signaling may provoke insulin resistance through diverse cellular events including the inhibition of insulin signaling [17,[27][28][29]. Taken together, we could postulate that one of the reasons of 17b-estradiol-induced insulin resistance might be resulted from the increase in resistin but decrease in adiponectin levels, which requires further confirmation by in vivo studies.…”

Section: Discussionmentioning

confidence: 81%

Effects of 17β-estradiol and progesterone on the production of adipokines in differentiating 3T3-L1 adipocytes: Role of Rho-kinase

et al. 2015

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Section: Discussionmentioning

confidence: 81%

Effects of 17β-estradiol and progesterone on the production of adipokines in differentiating 3T3-L1 adipocytes: Role of Rho-kinase

et al. 2015

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“…The foam cells were stained for 20 minutes at 37 ℃ with oil red O working solution. The cells were washed with 60% isopropanol to remove the background color, after which the intracellular stained oil red O was eluted with isopropanol and the optical density (OD) of the solution at 520 nm was measured using a kinetic microplate reader (Gene Company Limited, Hong Kong, China) 23) . The OD values were proportional to the amount of intracellu-…”

Section: Quantitative Analysis Of Foam Cell Formationmentioning

confidence: 99%

Involvement of TLR4 in Oxidized LDL/β2GPI/Anti-β2GPI-Induced Transformation of Macrophages to Foam Cells

Zhang

Xie

Zhou

et al. 2014

JAT

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Aim: It has been reported that oxidized low-density lipoprotein (oxLDL) forms a stable and non-dissociable complex with β2-glycoprotein I (β2GPI) and that IgG anti-β2GPI autoantibodies are able to recognize this complex, thus facilitating macrophage-derived foam cell formation in patients with antiphospholipid syndrome (APS). However, the immunopathological mechanisms of oxLDL/β2GPI complexes in promoting foam cell formation are not fully understood. In this study, we examined the role of toll-like receptor 4 (TLR4) in the oxLDL/β2GPI/anti-β2GPI complex-induced transformation of mouse peritoneal macrophages to foam cells.

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“…To date, numerous compounds that promote adipocyte differentiation have been found, including lecithin (Zhang et al, 2009), cilostazol , sakuranetin (Saito et al, 2008), phloretin (Hassan et al, 2007), magnolol (Choi et al, 2009), catechin (Shin et al, 2009), ginsenosides (Niu et al, 2009;Shang et al, 2007), fl avanone (Saito et al, 2009), raloxifene (Murase et al, 2006), progestins (Rondinone et al,1992), diallyl disulfi de (Lee et al, 2007b), emodin and nobiletin (Saito et al, 2007). More recently, Kim et al (2008) explored a wide variety of natural marine products for screening the PPARα/γ transcriptional activity, thereby fi nding two novel PPARα/γ dual agonists, sargaquinoic acid and sargahydroquinoic acid.…”

Section: Introductionmentioning

confidence: 99%

Extract of Chaga mushroom (Inonotus obliquus) stimulates 3t3‐l1 adipocyte differentiation

Joo

Kim

Yun

2010

Phytotherapy Research

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Chaga mushroom (Inonotus obliquus) has long been used as a folk medicine due to its numerous biological functions such as antibacterial, antiallergic, antiinflammatory and antioxidative activities. In the present study, it was found that the I. obliquus hot water extract (IOWE) activated adipogenesis of 3T3-L1 preadipocytes. Even in the absence of adipogenic stimuli by insulin, the IOWE strongly induced adipogenesis of 3T3-L1 preadipocytes. The major constituent of IOWE was glucose-rich polysaccharides with a molecular mass of 149 kDa. IOWE enhanced the differentiation of 3T3-L1 preadipocytes, increasing TG (triacylglycerol) accumulation that is critical for acquisition of the adipocyte phenotype, in a dose-dependent manner. IOWE stimulated gene expression of C/EBPα (CCAAT/enhancer-binding protein α) and PPARγ (peroxisome proliferator-activated receptors γ) during adipocyte differentiation, and induced the expression of PPARγ target genes such as aP2 (adipocyte protein 2), LPL (lipoprotein lipase) and CD36 (fatty acid translocase). Immunoblot analysis revealed that IOWE increased the expression of adipogenic makers such as PPARγ and GLUT4 (glucose transporter 4). The luciferase reporter assay demonstrated that IOWE did not exhibit PPARγ ligand activity. Although these results require further investigation, the ability of natural mushroom product to increase PPARγ transcriptional activities may be expected to be therapeutic targets for dyslipidemia and type 2 diabetes.

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Raloxifene promotes adipocyte differentiation of 3T3-L1 cells

Cited by 23 publications

References 14 publications

Effects of 17β-estradiol and progesterone on the production of adipokines in differentiating 3T3-L1 adipocytes: Role of Rho-kinase

Effects of 17β-estradiol and progesterone on the production of adipokines in differentiating 3T3-L1 adipocytes: Role of Rho-kinase

Involvement of TLR4 in Oxidized LDL/β2GPI/Anti-β2GPI-Induced Transformation of Macrophages to Foam Cells

Extract of Chaga mushroom (Inonotus obliquus) stimulates 3t3‐l1 adipocyte differentiation

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