Raman characterizations of red blood cells with β-thalassemia using laser tweezers Raman spectroscopy

Abstract

This study aimed to study the differences in Raman spectra of red blood cells (RBCs) among patients with β-thalassemia and controls using laser tweezers Raman spectroscopy (LTRS) system.A total of 33 patients with β-thalassemia major, 49 with β-thalassemia minor, and 65 controls were studied. Raman spectra of RBCs for each sample were recorded. Principal component analysis (PCA), one-way analysis of variance (ANOVA), and independent-sample t test were performed.The intensities of Raman spectra of β-thalassemia (major and minor) RBCs were lower than those of controls, especially at bands 1546, 1603, and 1619 cm–1. The intensity ratio of band 1546 cm–1 to band 1448 cm–1 demonstrated that there was a significant difference between the spectra of β-thalassemia major (mostly below 2.15) and those of controls. The spectra of controls could be well distinguished from those of β-thalassemia major using PCA. After normalization, the spectra of two different genotypes with β0/β0 mutations mainly overlapped, while those with β+/β+ mutations had lower intensity at bands 1546, 1603, and 1619 cm–1.The present study provided Raman characteristics of RBCs in patients with β-thalassemia major and supported the use of LTRS as a method for screening β-thalassemia major. The recognition rate for β-thalassemia minor needs to be further improved.

Spectroscopic Evaluation of Red Blood Cells of Thalassemia Patients with Confocal Microscopy: A Pilot Study

“…Therefore, it would be interesting to carry out another type of assay, referred to as the immuno-labeling of the cell membrane, which can only be carried out with the cells fixated; this would be helpful for detecting affectations in the structure of the cell membrane, which are not currently clearly determined. Nevertheless, another advantage of the combined use of the imaging techniques described in this work, unlike other classical tests (such as complete blood count, blood smears, or electrophoresis), is that it does not necessarily require reactives or the use of markers and fixating substances used to capture absorption spectra [30]. Thus, tests can be carried out in normal physiological conditions without the need to prepare hemolyzed serums and with a relatively low volume of RBCs.…”

Classification of Red Blood Cells using Principal Component Analysis Technique

“…Jia W. in 2018 was study the differences in Raman spectra of red blood cells (RBCs) among patients with β-thalassemia using PCA [20]. MU Ali in 2017 reported that Bioinformatics data is high dimensional, so that using PCA to selected of attributes of data for getting accurate results in classification, Prediction, Clustering and Pattern Extraction are useful techniques of data mining [21]. Sharma in 2012 was reported that there are no literatures yet with regard to the anemic RBCs feature selection that has been published [22].…”

Red blood cells under varying extracellular tonicity conditions: an optical tweezers combined with micro-Raman study

“…This spectroscopic tool have found high potential in health sciences for diagnosing cell abnormalities by probing the dynamic cellular processes at single cell level [20,21]. Researchers have already combined optical tweezers and Raman spectroscopy to gather vital information regarding the biochemical alterations occurring in a single biological cell to investigate various disease conditions [22][23][24][25].…”