Raman Chemical Imaging of Breast Tissue

Kline, Nicole J.; Treado, Patrick J.

doi:10.1002/(sici)1097-4555(199702)28:2/3<119::aid-jrs73>3.0.co;2-3

Cited by 88 publications

(53 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…34,38 The shift in the 680-cm peak originated from C-C twisting mode. 34,36,39 To further confirm that quercetin reacts with the boronic containing chemicals, the inorganic boric acid was tested. The spectra for quercetin and quercetin plus boric acid show the reaction between the 2 chemicals (Figure 6Fiii,iv).…”

Section: The Interaction Between Quercetin and Bortezomib Mg-262 Ormentioning

confidence: 99%

“…The spectral differences were also observed in the area of 1150 to 1080 cm, demonstrating C-C stretching. 34,39 In addition, peaks located in the region of 1450 to 1410 cm originate from symmetric stretching vibration of C-O, 35 and those in approximately 1500 m can be assigned to C-C stretching in rings. 34 Spectra presented in Figure 6Fiv,i, illustrated within the same region, indicate that similar changes take place in the reactions between quercetin with bortezomib and quercetin with boric acid.…”

Section: The Interaction Between Quercetin and Bortezomib Mg-262 Ormentioning

confidence: 99%

See 1 more Smart Citation

Dietary flavonoids inhibit the anticancer effects of the proteasome inhibitor bortezomib

Liu¹,

Agrawal²,

Movasaghi³

et al. 2008

Blood

View full text Add to dashboard Cite

Dietary flavonoids have many healthpromoting actions, including anticancer activity via proteasome inhibition. Bortezomib is a dipeptide boronate proteasome inhibitor that has activity in the treatment of multiple myeloma but is not effective in chronic lymphocytic leukemia (CLL). Although CLL cells are sensitive in vitro to bortezomib-induced apoptosis when cultured in medium, the killing activity was blocked when cultured in 50% fresh autologous plasma. IntroductionBortezomib (Velcade) is a first-in-class proteasome inhibitor developed specifically for use as an antineoplastic agent. It is the most potent antineoplastic agent for the treatment of relapsed, refractory multiple myeloma. 1 A total of 73% of patients with myeloma responded to treatment with bortezomib combined with pegylated liposomal doxorubicin. 2 However, only 4 of 15 patients with acute leukemia showed a decrease in blast count. 3 The efficacy of bortezomib in chronic lymphocytic leukemia (CLL) appears to be related to IgV(H) and BCL-6 mutational status, 4 down-regulation of CD23, and inactivation of Notch 2. 5 However, we have previously shown that all primary CLL cells were sensitive to bortezomib in vitro. Proteasome inhibition increased Bax protein accumulation and Bax activation in CLL cells. Bortezomib also increased the sensitivity of CLL cells to TNF-related apoptosis-inducing ligand-induced apoptosis. 6 Although the effectiveness of bortezomib killing of CLL cells in vitro has also been reported by other groups, 4,5 it did not display substantial antitumor activity in patients with CLL. 7,8 The basis of this differential activity of bortezomib on CLL cells in vivo and in vitro is unknown. Autologous plasma is capable of maintaining survival of CLL cells in vitro and conferring resistance to chemo-radiotherapy, 9 and albumin in plasma plays an important role in this survival mechanism. 10 We sought to identify factors in the blood, which could prevent bortezomib-mediated killing of leukemic cells in the circulation.Quercetin is one of the most abundant flavonoids in the human diet and is a potent antioxidant. 11 Quercetin, abundant in human plasma, noncovalently binds to serum albumin. 12,13 It contributes to the prevention of human diseases by promoting relaxation of cardiovascular smooth muscle and protects low-density lipoprotein from oxidation. 14 Recent studies found that quercetin can induce apoptosis by inhibiting proteasome activation, 15 causing G 2 /M-phase arrest 16 and increasing p21 expression. 17 Other dietary flavonoids, such as myricetin, kaempferol, and apigenin, also have similar functions to quercetin with respect to proteasome inhibition and apoptosis induction. 15 In this study, we demonstrate that human plasma affects killing by bortezomib and that the dietary flavonoids, especially quercetin, inhibit bortezomib-induced apoptosis in malignant B-cell lines and primary CLL cells. This inhibitory activity of quercetin was associated with complex formation with bortezomib. However, in myeloma cell lines, quercetin al...

show abstract

Section: The Interaction Between Quercetin and Bortezomib Mg-262 Ormentioning

confidence: 99%

Section: The Interaction Between Quercetin and Bortezomib Mg-262 Ormentioning

confidence: 99%

Dietary flavonoids inhibit the anticancer effects of the proteasome inhibitor bortezomib

Liu¹,

Agrawal²,

Movasaghi³

et al. 2008

Blood

View full text Add to dashboard Cite

show abstract

“…Light scattered by the sample is diffracted into individual wavelengths by a spectrograph and collected by a detector such as a CCD or CMOS sensor. 7 Raman systems can be coupled to a microscope and motorized stage for high-resolution imaging [8][9][10][11][12][13][14] or to a fiberoptic probe for bulk in vivo sampling. [15][16][17][18][19][20] Raman spectroscopy's independence from a specific sample thickness and lack of spectral interference from water make it an ideal technique for biomedical applications.…”

Section: Raman and Fourier Transform Infrared Spectroscopymentioning

confidence: 99%

Vibrational spectroscopy: a tool being developed for the noninvasive monitoring of wound healing

Crane¹,

Elster²

2012

J. Biomed. Opt.

View full text Add to dashboard Cite

Abstract. Wound care and management accounted for over 1.8 million hospital discharges in 2009. The complex nature of wound physiology involves hundreds of overlapping processes that we have only begun to understand over the past three decades. The management of wounds remains a significant challenge for inexperienced clinicians. The ensuing inflammatory response ultimately dictates the pace of wound healing and tissue regeneration. Consequently, the eventual timing of wound closure or definitive coverage is often subjective. Some wounds fail to close, or dehisce, despite the use and application of novel wound-specific treatment modalities. An understanding of the molecular environment of acute and chronic wounds throughout the wound-healing process can provide valuable insight into the mechanisms associated with the patient's outcome. Pathologic alterations of wounds are accompanied by fundamental changes in the molecular environment that can be analyzed by vibrational spectroscopy. Vibrational spectroscopy, specifically Raman and Fourier transform infrared spectroscopy, offers the capability to accurately detect and identify the various molecules that compose the extracellular matrix during wound healing in their native state. The identified changes might provide the objective markers of wound healing, which can then be integrated with clinical characteristics to guide the management of wounds.

show abstract

“…The potential benefits of using Raman spectroscopy to diagnose breast cancer have been studied by several research groups [14][15][16][17][18][19][20]. Visualization of breast tissue microstructural features is a key first step toward the development of a noninvasive Raman imaging technique for the identification and classification of breast cancer in a clinical environment.…”

Section: Breast Cancermentioning

confidence: 99%