Raman confocal microscopy and biophysics multiparametric characterization of the skin barrier evolution with age

Rigal, Aline; Michael‐Jubeli, Rime; Nkengne, Alex; Baillet‐Guffroy, Arlette; Bigouret, Armelle; Tfayli, Ali

doi:10.1002/jbio.202100107

Cited by 13 publications

(12 citation statements)

References 55 publications

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“…In older humans, keratin is in more unfolded state compared to younger humans [39], which was recently confirmed by Rigal et al [60]. Porcine skin, which is widely used as an ex vivo model of human skin in dermatological research, has more exposed secondary structure of keratin at ≈10–90% SC depth ( p < 0.01), and a more folded tertiary structure of keratin at ≈30–100% SC depth ( p < 0.05) in comparison with human SC in vivo, which determines binding of water inside the corneocytes [62].…”

Section: Resultsmentioning

confidence: 70%

“…and 50 y.o. age-groups) [39], which was recently confirmed by Rigal et al [60]. The lipid organization is changed toward decreasing of skin barrier function in the xerosis and atopic skin in vivo [32, 61].…”

Section: Resultsmentioning

confidence: 78%

“…age-groups) [39]. Increase of tightly bound, unbound, and total water content in elderly skin was also reported [60]. The reduction of bound water in the SC was reported for atopic skin in vivo [61].…”

Section: Resultsmentioning

confidence: 93%

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Current Views on Noninvasive in vivo Determination of Physiological Parameters of the Stratum Corneum Using Confocal Raman Microspectroscopy

Darvin

Schleusener

Lademann

et al. 2022

Skin Pharmacol Physiol

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Confocal Raman microspectroscopy is widely used in dermatology and cosmetology for analysis of the concentration of skin components (lipids, natural moisturizing factor molecules, water) and the penetration depth of cosmetic/medical formulations in the human stratum corneum (SC) in vivo. In recent years, it was shown that confocal Raman microspectroscopy can also be used for non-invasive in vivo depth-dependent determination of the physiological parameters of the SC, such as lamellar and lateral organization of intercellular lipids, folding properties of keratin, water mobility and hydrogen bonding states. The results showed that the strongest skin barrier function, which is primarily manifested by the orthorhombic organization of intercellular lipids, is provided at ≈20–40% SC depth, which is related to the maximal bonding state of water with surrounding components in the SC. The secondary and tertiary structures of keratin determine water binding in the SC, which is depth-dependent. This paper shows the technical possibility and advantage of confocal Raman microspectroscopy in non-invasive investigation of the skin and summarizes recent results on in vivo investigation of the human SC.

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Section: Resultsmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 78%

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Current Views on Noninvasive in vivo Determination of Physiological Parameters of the Stratum Corneum Using Confocal Raman Microspectroscopy

Darvin

Schleusener

Lademann

et al. 2022

Skin Pharmacol Physiol

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“…With age, the skin naturally has decreased lamellar body secretions, depletion of lipids, slower barrier repair, and increased TEWL [ 53 , 54 ]. Though the global stratum corneum water content decreases with age [ 55 ], the surface stratum corneum water content has been shown to be similar in both young and aged skin [ 56 ].…”

Section: Pathophysiology Associated With Diabetic Wound Healingmentioning

confidence: 99%

Diabetic Wound-Healing Science

et al. 2021

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Diabetes mellitus is an increasingly prevalent chronic metabolic disease characterized by prolonged hyperglycemia that leads to long-term health consequences. It is estimated that impaired healing of diabetic wounds affects approximately 25% of all patients with diabetes mellitus, often resulting in lower limb amputation, with subsequent high economic and psychosocial costs. The hyperglycemic environment promotes the formation of biofilms and makes diabetic wounds difficult to treat. In this review, we present updates regarding recent advances in our understanding of the pathophysiology of diabetic wounds focusing on impaired angiogenesis, neuropathy, sub-optimal chronic inflammatory response, barrier disruption, and subsequent polymicrobial infection, followed by current and future treatment strategies designed to tackle the various pathologies associated with diabetic wounds. Given the alarming increase in the prevalence of diabetes, and subsequently diabetic wounds, it is imperative that future treatment strategies target multiple causes of impaired healing in diabetic wounds.

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“…A recent study further analyzed water content in different stratum corneum layers, revealing similar amounts at the surface. At the same time, the authors found higher water content in deep parts of the stratum corneum and viable epidermis in aged skin [ 3 ]. This is explained by an age-dependent change in the amount of bound and unbound water.…”

mentioning

confidence: 99%