Raman microspectroscopy differentiates perinatal pathogens on ex vivo infected human fetal membrane tissues

Ayala, Oscar D.; Doster, Ryan S.; Manning, Shannon D.; O’Brien, C.; Aronoff, David M.; Gaddy, Jennifer A.; Mahadevan‐Jansen, Anita

doi:10.1002/jbio.201800449

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…Screening for colonization and intrapartum prophylaxis with antibiotics decreases the incidence of early onset neonatal sepsis 99 . GBS biofilms on fetal membranes have been observed 100 and it is one of the most commonly cultured bacteria in the setting of intra-amniotic infections 97 . GBS colonizes the genital tract in ~10–30% of women 101 .…”

Section: Torch Pathogensmentioning

confidence: 99%

Infections at the maternal–fetal interface: an overview of pathogenesis and defence

2021

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Infections are a major threat to human reproductive health, and infections in pregnancy can cause prematurity or stillbirth, or can be vertically transmitted to the fetus leading to congenital infection and severe disease. The acronym ‘TORCH’ ( Toxoplasma gondii , other, rubella virus, cytomegalovirus, herpes simplex virus) refers to pathogens directly associated with the development of congenital disease and includes diverse bacteria, viruses and parasites. The placenta restricts vertical transmission during pregnancy and has evolved robust mechanisms of microbial defence. However, microorganisms that cause congenital disease have likely evolved diverse mechanisms to bypass these defences. In this Review, we discuss how TORCH pathogens access the intra-amniotic space and overcome the placental defences that protect against microbial vertical transmission.

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Section: Torch Pathogensmentioning

confidence: 99%

Infections at the maternal–fetal interface: an overview of pathogenesis and defence

2021

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“…Vaginal infections caused by group B Streptococcus (GBS), Escherichia coli , Bacteroides species, C. trachomatis , and N. gonorrhoeae can ascend to the genital tract and intraamniotic fluid causing chorioamnionitis ( Galinsky et al., 2013 ; Jain et al., 2022 ). Infections caused by ascending genito-urinary tract pathogens are typically polymicrobial ( Mendz et al., 2013 ) and often associated with microbial biofilm and antimicrobial cervical mucous plug to reach the intra-amniotic fluid or maternal-fetal interface and induce inflammation locally, which then endangers the fetus due to aberrant inflammation at the fetal membrane ( Ayala et al., 2019 ). There is no clear evidence of how dysbiotic flora crosses the maternal barriers to reach the fetus, but GBS and E. coli are the most common pathogens found in the placenta and late-onset sepsis in neonates ( Wilkie et al., 2019 ; Glaser et al., 2021 ).…”

Section: Maternal Infections During Pregnancymentioning

confidence: 99%

Infections and Pregnancy: Effects on Maternal and Child Health

Kumar

Saadaoui

Khodor

2022

Front. Cell. Infect. Microbiol.

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Pregnancy causes physiological and immunological adaptations that allow the mother and fetus to communicate with precision in order to promote a healthy pregnancy. At the same time, these adaptations may make pregnant women more susceptible to infections, resulting in a variety of pregnancy complications; those pathogens may also be vertically transmitted to the fetus, resulting in adverse pregnancy outcomes. Even though the placenta has developed a robust microbial defense to restrict vertical microbial transmission, certain microbial pathogens have evolved mechanisms to avoid the placental barrier and cause congenital diseases. Recent mechanistic studies have begun to uncover the striking role of the maternal microbiota in pregnancy outcomes. In this review, we discuss how microbial pathogens overcome the placental barrier to cause congenital diseases. A better understanding of the placental control of fetal infection should provide new insights into future translational research.

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“…According to the authors, however, the lack of FTIR spectral libraries is the major limitation in the FTIR-based identification of Candida strains, [49][50] which is line with bacterial strains. [13][14][51][52] 8…”

Section: Vibrational Spectroscopy In Fungal Infectionsmentioning

confidence: 99%

Vibrational spectroscopy and its future applications in microbiology

Jansson

Kögler

Hörkkö

et al. 2021

Applied Spectroscopy Reviews

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Vibrational spectroscopic techniques, namely Fourier transform infrared (FTIR) and Raman spectroscopy, are based on the study of molecular vibrations, and they are complementary techniques to each other. This review provides an overview of the vibrational spectroscopic techniques applied in microbiology during the past decade. In addition, future applications of the elaborated spectroscopic techniques will be highlighted. The results of this review show that both FTIR and Raman spectroscopy are promising alternatives to conventional diagnostic approaches because they provide label-free and noninvasive bacterial detection, identification, and antibiotic susceptibility testing in a single step. Cost-effective, accurate, and rapid tests are needed in order to improve diagnostics and patient care, to decrease the use of unnecessary antimicrobial agents, to prevent resistant microbials, and to decrease the overall burden of outbreaks. Prior to that, however, the presented approaches need to be validated in a clinical workflow against the conventional diagnostic approaches.

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Raman microspectroscopy differentiates perinatal pathogens on ex vivo infected human fetal membrane tissues

Cited by 6 publications

References 24 publications

Infections at the maternal–fetal interface: an overview of pathogenesis and defence

Infections at the maternal–fetal interface: an overview of pathogenesis and defence

Infections and Pregnancy: Effects on Maternal and Child Health

Vibrational spectroscopy and its future applications in microbiology

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