Raman microspectroscopy of human coronary atherosclerosis: Biochemical assessment of cellular and extracellular morphologic structures in situ

Buschman, H.P.J.; Deinum, Geurt; Motz, Jason T.; Fitzmaurice, Maryann; Kramer, John; Laarse, Arnoud van der; Bruschke, Albert V.G.; Feld, Michael S.

doi:10.1016/s1054-8807(01)00064-3

Cited by 135 publications

(112 citation statements)

References 47 publications

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“…Some were commonly used in skin and non-skin models. For instance, collagen and triolein are known to be important contributors to the RS signal of breast, gastric, and artery tissues [17,19,20]. We demonstrated these two components also played an important role for fitting in vivo skin data.…”

Section: Discussionmentioning

confidence: 78%

“…The building blocks of our model are Raman active components extracted from skin in situ. Previous biophysical models used model components either measured directly from synthetic/purified chemicals [9,[15][16][17][18], or extracted from tissue sections in situ [19][20][21]. The advantage of using synthetic/purified chemicals as model components is that they can be easily measured without the need for Raman micro-imaging.…”

Section: Introductionmentioning

confidence: 99%

“…This concept has been applied successfully to a previous Raman model of excisional skin biopsies [9] and has given us some prior knowledge about skin composition. Other groups used in situ constituents to build biophysical models of other disease processes, such as coronary atherosclerosis [19], breast cancer [20], and brain tumor [21]. Here, we expand upon this approach by developing a biophysical human skin model using in situ skin constituents.…”

Section: Introductionmentioning

confidence: 99%

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Raman active components of skin cancer

Feng¹,

Moy²,

Nguyen³

et al. 2017

Biomed. Opt. Express

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Raman spectroscopy (RS) has shown great potential in noninvasive cancer screening. Statistically based algorithms, such as principal component analysis, are commonly employed to provide tissue classification; however, they are difficult to relate to the chemical and morphological basis of the spectroscopic features and underlying disease. As a result, we propose the first Raman biophysical model applied to in vivo skin cancer screening data. We expand upon previous models by utilizing in situ skin constituents as the building blocks, and validate the model using previous clinical screening data collected from a Raman optical fiber probe. We built an 830nm confocal Raman microscope integrated with a confocal laser-scanning microscope. Raman imaging was performed on skin sections spanning various disease states, and multivariate curve resolution (MCR) analysis was used to resolve the Raman spectra of individual in situ skin constituents. The basis spectra of the most relevant skin constituents were combined linearly to fit in vivo human skin spectra. Our results suggest collagen, elastin, keratin, cell nucleus, triolein, ceramide, melanin and water are the most important model components. We make available for download (see supplemental information) a database of Raman spectra for these eight components for others to use as a reference. Our model reveals the biochemical and structural makeup of normal, nonmelanoma and melanoma skin cancers, and precancers and paves the way for future development of this approach to noninvasive skin cancer diagnosis.

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Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Raman active components of skin cancer

Feng¹,

Moy²,

Nguyen³

et al. 2017

Biomed. Opt. Express

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“…Such tools should be capable of diagnosing the "structural status" of the aorta and giving an indication as to whether its replacement is imminent. Raman microscopy has been used to analyze atheroma [3,4] but no extensive study on the composition of the three layers of the tissue has been carried out. Studies concerning FTIR microscopy are even scarcer.…”

Section: Introductionmentioning

confidence: 99%

FTIR protein secondary structure analysis of human ascending aortic tissues

Bonnier

Rubin

Debelle

et al. 2008

Journal of Biophotonics

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Journal of BIOPHOTONICSThe advent of moderate dilatations in ascending aortas is often accompanied by structural modifications of the main components of the aortic tissue, elastin and collagen. In this study, we have undertaken an approach based on FTIR microscopy coupled to a curve-fitting procedure to analyze secondary structure modifications in these proteins in human normal and pathological aortic tissues. We found that the outcome of the aortic pathology is strongly influenced by these proteins, which are abundant in the media of the aortic wall, and that the advent of an aortic dilatation is generally accompanied by a decrease of parallel b-sheet structures. Elastin, essentially composed of b-sheet structures, seems to be directly related to these changes and therefore indicative of the elastic alteration of the aortic wall. Conventional microscopy and confocal fluorescence microscopy were used to compare FTIR microscopy results with the organization of the elastic fibers present in the tissues. This in-vitro study on 6 patients (three normal and three pathologic), suggests that such a spectroscopic marker, specific to aneurismal tissue characterization, could be important information for surgeons who face the dilemma of moderate aortic tissue dilatation of the ascending aortas.Infrared spectroscopy and imaging was applied to analyse Human thoracic ascending aortas with the aim to highlight the protein secondary structure reorganisation after an aneurism outcome. We used a curve-fitting procedure to visualise modifications in the protein spectral profile where alteration of b structures in pathological aortas could be detected. Our spectroscopic observations on tissue sections corroborated with confocal fluorescence microscopy clearly demonstrating that elastic fibres of aortic wall are strongly altered. Elastin appears as the privileged target during the pathological process.

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“…The range of possible light-matter interaction mechanism, that includes spectrally dependent absorption, Raman scattering (23,24) and second (and higher) harmonic generation (SHG) (25)(26)(27), can all be used to obtain specific biochemical information in a biomedical context. These optical phenomena form the basis for near-IR pulse oximeters, SHG microscopes (25,27) and coherent Anti-Stokes Raman Scattering (CARS) microscopes (28)(29)(30).…”

Section: Introductionmentioning

confidence: 99%

Molecular Contrast Optical Coherence Tomography: A Review

Yang¹

2004

Photochem Photobiol

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This article reviews the current state of research on the use of molecular contrast agents in optical coherence tomography (OCT) imaging techniques. After a brief discussion of the basic principle of OCT and the importance of incorporating molecular contrast agent usage into this imaging modality, we shall present an overview of the different molecular contrast OCT (MCOCT) methods that have been developed thus far. We will then discuss several important practical issues that define the possible range of contrast agent choice, the design criteria for engineered molecular contrast agent and the implementability of a given MCOCT method for clinical or biological applications. We will conclude by outlining a few areas of pursuit that deserve a greater degree of research and development.

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Raman microspectroscopy of human coronary atherosclerosis: Biochemical assessment of cellular and extracellular morphologic structures in situ

Cited by 135 publications

References 47 publications

Raman active components of skin cancer

Raman active components of skin cancer

FTIR protein secondary structure analysis of human ascending aortic tissues

Molecular Contrast Optical Coherence Tomography: A Review

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