Raman spectra analysis of human blood protein fractions using the projection on latent structures method

Lykina, Anastasiya A.; Artemyev, Dmitry N.; Bratchenko, Ivan А.; Khristoforova, Yulia А.; Myakinin, Oleg; Кузьмина, Т. П.; Давыдкин, И. Л.; Zakharov, Valery P.

doi:10.18287/1613-0073-2017-1900-64-68

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…Raman peaks at 570-700 cm −1 , 1120 cm −1 , 1550 cm −1 are the most informative peaks in whole blood that correspond to hemoglobin and they are not found at blood plasma spectra. Peaks at 1002 cm −1 , 1227 cm −1 , 1440 cm −1 , and 1630 cm −1 are the largest VIP value and they can be found both in blood plasma and whole blood that correspond to the peaks of albumin and globulin [41].…”

Section: Protein Analysis In Biomedical Fluids Using Raman Spectroscopymentioning

confidence: 99%

The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: A review

Rohman

Windarsih

Lukitaningsih

et al. 2020

BSI

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Fourier transform infrared (FTIR) and Raman spectroscopy are complementary techniques, typically called vibrational spectroscopy. Both techniques allow simple, rapid, non-destructive, specific, providing fingerprint spectra, and real-time analytical method for analysis of molecules in different states. Besides, these methods are simple without any excessive sample pre-treatment, therefore, they are sometimes called as "green analytical methods". Biofluids have several biomolecules such as lipid, protein, nucleic acids, and carbohydrates. These biomolecules can be used as biomarkers to detect some types of diseases, since biomolecules are in direct contact with the human organs. FTIR and Raman spectra of biofluids are complex in nature, therefore sophisticated statistical techniques, known as chemometrics, must be used to solve the analytical problems related to quantitative analysis purposes. The objective of this review is to show the capability of FTIR and Raman spectroscopic techniques in combination with chemometrics techniques to analyze the biomolecules in biofluids through an extensive literature review. During performing this review, several databases in Science citation index, Scopus PubMed, and Google Scholar related to the topics are identified and downloaded. With the present review, it is known that FTIR and Raman techniques are rapid method for screening certain diseases by identifying the level changes of some biomolecules. In the future, this method will be widely used for clinicians as new diagnostic tools for many diseases.

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Section: Protein Analysis In Biomedical Fluids Using Raman Spectroscopymentioning

confidence: 99%

The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: A review

Rohman

Windarsih

Lukitaningsih

et al. 2020

BSI

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show abstract

“…5A. Table 4 lists the Raman peaks for the functional groups in the protein based on the literature on whole blood and proteins [30,31]. The band at 1636 cm −1 was identified as the protein amide I band, which was related to the protein backbone structure.…”

Section: Raman Specta Of the Porcine Blood Hydrogelsmentioning

confidence: 99%

Mechanism for formation of porcine blood hydrogels used as additives in the mortar of traditional Chinese architectural painting

Cheng,

Zhu,

Zhang

et al. 2024

Herit Sci

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Ancient Chinese architecture drawing is a unique form of ancient Chinese art. Porcine blood hydrogels are distinctive ingredients used to prepare the composite material of the substrate layer for architecture drawing. This investigation was focused on the mechanism for porcine blood hydrogel formation. Based on the traditional Chinese recipe for the preparation of porcine blood-lime mortar, samples with different ratios of porcine blood and lime water were prepared, and the molecular-scale interactions between the lime water and proteins in the porcine blood were examined with FTIR spectroscopy, Raman spectroscopy, XRD, XPS, fluorescence spectroscopy, contact angle and rheology studies. The mechanical properties and morphological features of the samples were detected with a rheometer, universal material testing machine and SEM. The results indicated that an appropriate amount of lime water was required for gelation of the porcine blood. With the appropriate alkalinity, sufficient carboxyl groups on the fibrin chains were deprotonated, and coordination with Ca2+ ions formed enough cross-links for support networks within the structure of the porcine blood hydrogel. Complexation of deprotonated carboxyl groups on the fibrin chains with Ca2+ ions led to the formation of a hydrophobic surface due alterations of the fibrin conformation and increased the adhesive properties of the hydrogel. With the hydrogel used as an ingredient in the preparation of a substrate layer composite, it is suggested that the hydrophobic surface of the hydrogel facilitated mixing with hydrophobic tung oil during preparation of the composite material, and strong adhesion of the hydrogel increased the mechanical strength and crack resistance of the substrate layer.

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Microfluidically Integrated SERS Active Cell Trap Array for Sensitive Analysis of Red Blood Cells

Hakkel

Rigó

Vereš

et al. 2019

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems &Amp; Eurosensors XXXIII (TRANSDUCERS &Am

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Raman spectra analysis of human blood protein fractions using the projection on latent structures method

Cited by 4 publications

References 15 publications

The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: A review

The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: A review

Mechanism for formation of porcine blood hydrogels used as additives in the mortar of traditional Chinese architectural painting

Microfluidically Integrated SERS Active Cell Trap Array for Sensitive Analysis of Red Blood Cells

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