Raman spectroscopic detection of early stages in DMBA-induced tumor evolution in hamster buccal pouch model: an exploratory study

Ghanate, Avinash D.; Kumar, Gaurav; Talathi, Sneha; Maru, Girish B.; Krishna, C. Murali

doi:10.1117/12.897714

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…A total of 115 six‐ to eight‐week‐old Golden Syrian male hamsters ( Mesocricetus auratus ) were used in the study. Oral carcinogenesis was induced in 70 HBP (treated group) using standard DMBA carcinogenesis protocols . Briefly, 0.5% DMBA (Sigma‐Aldrich, purity 95%) dissolved in groundnut oil (Dhara, India) was topically applied using a paintbrush (Camlin, no.…”

Section: Methodsmentioning

confidence: 99%

Raman spectroscopy in experimental oral carcinogenesis: investigation of abnormal changes in control tissues

Kumar

Bhattacharjee

Pandey

et al. 2016

J Raman Spectroscopy

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Oral cancer is a major cause of mortality in South Asian men owing to rampant tobacco abuse. Cancers are also reported in non‐tobacco habitués, especially women, attributed to chronic irritations from irregular/sharp teeth, improper fillings, and poorly fit dentures. Conventional screening approaches are shown to be effective for high‐risk groups (tobacco/alcohol habitués). Raman spectroscopy (RS) is being extensively explored as an alternate/adjunct tool for diagnosis and management of oral cancers. In a previous Raman study on sequential oral carcinogenesis using hamster buccal pouch model, misclassifications between spectra from control and carcinogen [7,12‐dimethylbenz(a)anthracene (DMBA)]‐treated tissues were observed. Histopathology of some control tissues suggested pathological changes, attributable to repeated forceps‐induced irritations/trauma during animal handling. To explore these changes, in the present study, we recorded spectra from three different types of controls – vehicle control (n = 45), vehicle contralateral (n = 45), and DMBA contralateral (n = 70) – exposed to varying degree of forceps handling, along with DMBA‐treated pouches (n = 70) using a 14‐week carcinogenesis protocol. Spectra certified on the basis of histopathology and abnormal cell proliferation (cyclin D1 expression) were used to build models that were evaluated by independent test spectra from an exclusive set of DMBA‐treated and control animals. Many DMBA‐contralateral, vehicle‐control, and vehicle‐contralateral spectra were identified as higher pathologies, which subsequently corroborated with histopathology/cyclin D1 expression. Repeated forceps‐mediated injuries/irritations, during painting and animal handling, may elicit inflammatory responses, leading to neoplasm. The findings of the study suggest that RS could identify micro‐changes. Further, RS‐based in vivo imaging can serve as a promising label‐free tool for screening even in the non‐habitué population where conventional screening is shown to be not effective. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Methodsmentioning

confidence: 99%

Raman spectroscopy in experimental oral carcinogenesis: investigation of abnormal changes in control tissues

Kumar

Bhattacharjee

Pandey

et al. 2016

J Raman Spectroscopy

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“…Hamster buccal pouch (HBP) is a widely used model for experimental oral carcinogenesis, [26][27][28] owing to attributes such as cancer progression through stages similar to human oral carcinogenesis and 100% incidence of tumors in 14 weeks on treatment with 7,12-dimethylbenz(a)anthracene (DMBA). 29,30 In fact, feasibility of RS-based classification of frank tumors 31 as well as carcinogen-induced variations in HBP 32 on a limited sample size has already been reported.…”

Section: Introductionmentioning

confidence: 99%

Raman Spectroscopy of Experimental Oral Carcinogenesis

Kumar

Bhattacharjee

Ingle

et al. 2016

Technol Cancer Res Treat

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Oral cancers suffer from poor 5-year survival rates, owing to late detection of the disease. Current diagnostic/screening tools need to be upgraded in view of disadvantages like invasiveness, tedious sample preparation, long output times, and interobserver variances. Raman spectroscopy has been shown to identify many disease conditions, including oral cancers, from healthy conditions. Further studies in exploring sequential changes in oral carcinogenesis are warranted. In this Raman spectroscopy study, sequential progression in experimental oral carcinogenesis in Hamster buccal pouch model was investigated using 3 approachesex vivo, in vivo sequential, and in vivo follow-up. In all these studies, spectral changes show lipid dominance in early stages while later stages and tumors showed increased protein to lipid ratio and nucleic acids. On similar lines, early weeks of 7,12-dimethylbenz(a)anthracene-treated and control groups showed higher overlap and low classification. The classification efficiency increased progressively, reached a plateau phase and subsequently increased up to 100% by 14 weeks. The misclassifications between treated and control spectra suggested some changes in controls as well, which was confirmed by a careful reexamination of histopathological slides. These findings suggests Raman spectroscopy may be able to identify microheterogeneity, which may often go unnoticed in conventional biochemistry wherein tissue extracts are employed, as well as in histopathology. In vivo findings, quite comparable to gold-standard supported ex vivo findings, give further proof of Raman spectroscopy being a promising label-free, noninvasive diagnostic adjunct for future clinical applications.

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“…28,29 The location of tumors within the buccal pouch of the hamster correlates with tobacco smoking, as well as the site, in which the betel quid is placed for prolonged durations. 30 Therefore, the DMBA-induced oral carcinogenesis model is highly suitable for the study of the relationship between the morphological/biochemical alterations of the optical spectra and also the assessment of the early diagnosis of pre-malignant and malignant oral lesions coupled with appropriate curative treatment. 27,31 Hence, the present study was designed to evaluate the chemopreventive efficacy of the prepared silibininloaded nanoparticles (SILNPs) in comparison with free SIL for monitoring endogenous uorophore emission and to quantify the metabolic changes in the redox state against DMBA-induced oral carcinogenesis using a uorescence spectroscopic technique.…”

Section: Introductionmentioning

confidence: 99%

Monitoring the metabolic response to nanoencapsulated silibinin treatment in DMBA-induced oral carcinogenesis using endogenous fluorescence

et al. 2014

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Raman spectroscopic detection of early stages in DMBA-induced tumor evolution in hamster buccal pouch model: an exploratory study

Cited by 3 publications

References 14 publications

Raman spectroscopy in experimental oral carcinogenesis: investigation of abnormal changes in control tissues

Raman spectroscopy in experimental oral carcinogenesis: investigation of abnormal changes in control tissues

Raman Spectroscopy of Experimental Oral Carcinogenesis

Monitoring the metabolic response to nanoencapsulated silibinin treatment in DMBA-induced oral carcinogenesis using endogenous fluorescence

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