Raman spectroscopic evaluation of human serum using metal plate and 785- and 1064-nm excitation lasers

Abstract

In this study, we utilized a stainless steel (SUS304) plate for measuring the Raman scattering spectra of body fluid samples. Using this stainless steel plate, we recorded the Raman scattering spectra of 99.5% ethanol and human serum samples by performing irradiation with 785- and 1064-nm lasers. Raman scattering spectra with intensities equal to or greater than those reported previously were obtained. In addition, the Raman scattering spectra acquired using the 1064-nm laser were less influenced by autofluorescence than those obtained via use of the shorter-wavelength laser. Moreover, the shapes of the spectra did not show any dependence on integration time, and denaturation of the samples was minimal. Our method, based on 1064-nm laser and the stainless steel plate, provides performance equal to or better than the methods reported thus far for the measurement of Raman scattering spectra from liquid samples. This method can be employed to rapidly evaluate the components of serum in liquid form without using surface-enhanced Raman scattering.

Serum Raman spectroscopy can be used to screen patients with early rheumatoid arthritis

“…The Raman spectrometer used in this study was designed by the Sichuan Institute of Brain Science and Brain-like Intelligence. The laser wavelength was set to 785 nm, the power was 50 mW, and the exposure time was 1 s [15,16]. Raman scattering was excited and Raman spectra in 400-1800 cm À1 were recorded.…”

μ-Raman Determination of Essential Oils’ Constituents from Distillates and Leaf Glands of Origanum Plants

“…In a study of ethanol determination in urine samples [ 15 ], a highly reflective gold layer carrier, a cuvette with a lid and a gold coated slide with a cavity and covered with cling film were tested, in order to find the appropriate carrier regarding the minimum required sample volume and the prevention of evaporation. Similarly, different fluid sample carriers were screened for Raman measurements of human serum, initially tested with ethanol, including glass slides with a hole to trap the liquid under a coverslip, coverslip-covered O-rings on glass or quartz slides and the eventually proposed, mirror-finished stainless-steel plate with holes [ 16 ]. In the existing literature related to the analysis of EOs with Raman spectroscopy, the usage of various types of sample carriers has been reported, such as aluminum substrates [ 17 ], metal rings [ 10 , 18 ], quartz cells [ 13 ] and NMR glass tubes [ 19 ].…”