Raman spectroscopy investigation of various saturated monoacid triglycerides

Bresson, Serge; Marssi, M. El; Khelifa, B.

doi:10.1016/j.chemphyslip.2004.12.009

Cited by 57 publications

(65 citation statements)

References 17 publications

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“…Any presence of unsaturated carbon bonds in the methylene chain will induce additional peaks at $1,265 cm À1 (d ( --CH)), $1,650 cm À1 (n(C --C)), and 3,000 cm À1 (n( --CH)) compared to a saturated chain. However, the position of carbon-carbon double bonds, when they are present, does not alter the spectrum to a large extent (Bresson et al, 2005). For the triglycerides present in green micro-algae, of which fatty acid chains are predominantly long and unsaturated (Guschina and Harwood, 2006), we expect the Raman spectra to be largely similar.…”

Section: Chemical Identificationmentioning

confidence: 94%

Micro‐Raman spectroscopy of algae: Composition analysis and fluorescence background behavior

Huang

Beal

Cai

et al. 2010

Biotech & Bioengineering

129

106

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Preliminary feasibility studies were performed using Stokes Raman scattering for compositional analysis of algae. Two algal species, Chlorella sorokiniana (UTEX #1230) and Neochloris oleoabundans (UTEX #1185), were chosen for this study. Both species were considered to be candidates for biofuel production. Raman signals due to storage lipids (specifically triglycerides) were clearly identified in the nitrogen-starved C. sorokiniana and N. oleoabundans, but not in their healthy counterparts. On the other hand, signals resulting from the carotenoids were found to be present in all of the samples. Composition mapping was conducted in which Raman spectra were acquired from a dense sequence of locations over a small region of interest. The spectra obtained for the mapping images were filtered for the wavelengths of characteristic peaks that correspond to components of interest (i.e., triglyceride or carotenoid). The locations of the components of interest could be identified by the high intensity areas in the composition maps. Finally, the time evolution of fluorescence background was observed while acquiring Raman signals from the algae. The time dependence of fluorescence background is characterized by a general power law decay interrupted by sudden high intensity fluorescence events. The decreasing trend is likely a result of photo-bleaching of cell pigments due to prolonged intense laser exposure, while the sudden high intensity fluorescence events are not understood.

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Section: Chemical Identificationmentioning

confidence: 94%

Micro‐Raman spectroscopy of algae: Composition analysis and fluorescence background behavior

Huang

Beal

Cai

et al. 2010

Biotech & Bioengineering

129

106

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show abstract

“…Two major vibrational spectroscopy-based techniques have been used to distinguish the molecular structure of TG polymorphs, namely infrared spectroscopy (Kobayashi et al, 1986;Kodali et al, 1989;Yano and Sato, 1999;Dohi et al, 2002;Chang et al, 2005;Yang et al, 2005) and Raman spectroscopy (Koyama and Ikeda, 1980;Simpson and Hagemann, 1982;Simpson, 1983;Hernqvist, 1984;Kobayashi et al, 1986;Kodali et al, 1989;Sprunt et al, 2000;Dohi et al, 2002;Yang et al, 2005;Meng et al, 2005; Bresson et al, 2005Bresson et al, , 2006Da Silva and Rousseau, 2008). These techniques can also provide substantial structural and thermodynamic information (Da Silva and Rousseau, 2008).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the three major polymorphic forms and liquid state of tristearin by Raman spectroscopy

Silva

Bresson

Rousseau

2009

Chemistry and Physics of Lipids

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“…On the other hand, triglycerides have characteristic Raman bands [76][77][78] and moss spores are able to store triglycerides [79,80]. Hence, it would be expected that triglyceride signals should be clear in the Raman spectrum of the F. hygrometrica spores.…”

Section: Raman Spectroscopic Analysismentioning

confidence: 99%

Survival of Moss Reproductive Structures under Simulated Martian Environmental Conditions and Extreme Thermal Stress: Vibrational Spectroscopic Study and Astrobiological Implications

Gómez¹,

Estébanez²

2016

Astrobiol Outreach

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The principal goal of astrobiology is the search for extraterrestrial life forms. A key aspect is the study of the ability of different kinds of terrestrial organisms to support simulated extraterrestrial environmental conditions. Mosses are multicellular green plants, poorly studied from an astrobiological perspective. In this paper, we report experimental results obtained using two species of moss, which demonstrate that both the spores of the moss Funaria hygrometrica as well as the desiccated vegetative gametophyte shoots of the moss Tortella squarrosa (=Pleurochaete squarrosa) were capable of resisting Simulated Martian Environmental Conditions (SMEC): Mars simulated atmospheric composition 99.9% CO 2 , and 0.6% H 2 O with a pressure of 7 mbars, -73 º C and UV irradiation of 30 mW cm -2 in a wavelength range of 200-400 nm under a limited short time of exposition of 2 hours. After being exposed to SMEC and then transferred to an appropriate growth medium, the F. hygrometrica spores germinated, producing typical gametophyte protonemal cells and leafy shoots. Likewise, detached leaves from SMEC-exposed gametophyte shoots of T. squarrosa retained the ability to produce new protonemata and shoots under suitable growth conditions. Furthermore, we studied the tolerance of these moss structures to a thermal stress of 100 °C for 1 h; in both cases the spores and shoots were capable of resisting this heat treatment. Our study using FT-Raman and FT-IR vibrational spectroscopy demonstrated that neither spores nor shoots apparently suffered significant damage in their biomolecular makeup after being subject to these stress treatments. The implications of these findings for the search of life on Mars are discussed.

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Raman spectroscopy investigation of various saturated monoacid triglycerides

Cited by 57 publications

References 17 publications

Micro‐Raman spectroscopy of algae: Composition analysis and fluorescence background behavior

Micro‐Raman spectroscopy of algae: Composition analysis and fluorescence background behavior

Characterization of the three major polymorphic forms and liquid state of tristearin by Raman spectroscopy

Survival of Moss Reproductive Structures under Simulated Martian Environmental Conditions and Extreme Thermal Stress: Vibrational Spectroscopic Study and Astrobiological Implications

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