Raman spectroscopy method for the evaluation of bone bioimplants made using the "Lyoplast" technology from cadaveric and in vivo resected bone tissue

Тимченко, П. Е.; Timchenko, E. V.; Волова, Л. Т.; Dolgushkin, D. A.; Boltovskaya, V. V.; Frolov, O. O.

doi:10.1088/1742-6596/1038/1/012090

Cited by 9 publications

(3 citation statements)

References 6 publications

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“…For asymptomatic ONFH, the relationship between symptom onset and alcohol was reported. And it has been reported that the most important prognostic factor is the location and size of the lesion and that the collapse of the femoral head is extensively progressive [ 32 , 33 ]. Studies have reported that the habit of drinking large amounts of alcohol is a high risk for symptomatic progression of asymptomatic ONFH [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…Previous investigations proved that Raman spectroscopy can detect alterations in the chemical structure of the bone two major components, hydroxyapatite crystals and collagen matrix [ 30 , 31 , 32 ]. Building upon these findings, spectroscopic methods were made available for the detection of bone structural changes related to osteoporosis [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

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PCA-Assisted Raman Analysis of Osteonecrotic Human Femoral Heads

Ishimura

Zhu

Marin

et al. 2022

MPs

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Osteonecrosis of the femoral head (ONFH) occurs frequently in adolescents and young adults and causes progressive deformation and destruction of the hip joint and impairs standing and walking, resulting in a significant decrease in the quality of life of patients. In addition, studies have shown that a history of corticosteroid administration and heavy alcohol consumption are closely related to the occurrence of ONFH. However, the detailed mechanism by which steroid administration and alcohol consumption are associated with the development of the disease is still unknown. With many researches still ongoing and without a clear biological pathway for osteonecrosis, effective preventive measures cannot be taken. Therefore, the current focus of ONFH treatment is to establish an early diagnosis and treatment strategy. We obtained the femoral heads of four patients with steroidal ONFH and three patients with alcoholic ONFH. We then compared the femoral heads of steroidal and alcoholic osteonecrosis by analyzing them at the molecular level by Raman spectroscopy. Crystallographic changes (deformations) in the mineral phase and fraction of organic material respect to the total mass were then plotted as a function. We found that changes in bone composition in ONFH were different in steroidal and alcoholic ONFH. We conclude that this suggests that the developmental mechanisms of steroidal and alcoholic ONFH may follow different paths. We also noticed that while steroid seem to lead to a more marked degradation of the tissue, alcohol seem to affect also the quality of the healthy tissue.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

PCA-Assisted Raman Analysis of Osteonecrotic Human Femoral Heads

Ishimura

Zhu

Marin

et al. 2022

MPs

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“…It is rapidly arising as a bright device for biomedical investigations and clinical diagnosis, such as the detection of early-stage cancer, and it has been validated in countless ex vivo studies [ 4 , 5 , 6 ]. Recently, Raman spectroscopy has been applied to the investigation of bone quality in bioimplants [ 7 ]. Timchenko and his team performed a comparative analysis of the surfaces of mineralized and demineralized bone bioimplants.…”

Section: Introductionmentioning

confidence: 99%

Periodontal Disease Monitoring by Raman Spectroscopy of Phosphates: New Insights into Pyrophosphate Activity

Gatin,

Iordache,

Gatin

et al. 2023

Diagnostics

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(1) Background: The intent of this survey was to investigate the quality of the alveolar bone by revealing the different phases for calcified tissues independent of the medical history of the patient in relation to periodontal disease by means of Raman spectroscopy and then to correlate the results by suggesting a possible mechanism for the medical impairment; (2) Methods: The investigation was mainly based on Raman spectroscopy that was performed in vivo during surgery for the selected group of patients. The targeted peaks for the Raman spectra were according to the reference compounds (e.g., calcium phosphates, other phosphates); (3) Results: The variation in the intensity of the spectrum correlated to the specific bone constituents’ concentrations highlights the bone quality, while some compounds (such as pyrophosphate, PPi) are strongly related to the patient’s medical status, and they provide information regarding a physiological process that occurred in the calcified tissues. Moreover, bone sample fluorescence is related to the collagen (Col) content, enabling a complete evaluation of bone quality, revealing the importance of collagen matrix acting as a load-bearing element for Calcium phosphate (CaP) deposition during the complex bone mineralization process; (4) Conclusions: We highlight that Raman spectroscopy can be considered a viable investigative method for in vivo and rapid bone quality valuation through oral health monitoring.

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