Raman spectroscopy of advanced glycation end products (AGEs), possible markers for progressive retinal dysfunction

Pawlak, Anna; Beattie, J. Renwick; Glenn, Josephine V.; Stitt, Alan W.; McGarvey, John J.

doi:10.1002/jrs.2011

“…The Raman approach is ideally suited for investigating biological tissue and, in the eye, it has been used to analyze carotenoid content of the macula (25), assess the histochemistry of retina (DHA, monounsaturated fats, 4 proteins, DNA, heme, cytochrome c, and kynurenine) (26,27), detect ocular drugs (28), and assess lens structure (29,30). We have demonstrated previously the AGEs carboxymethyl-lysine (CML) and pentosidine in aging ocular tissues using Raman spectroscopy (31)(32)(33), but the aim of the present investigation has been to extend the scope of Raman scattering significantly as a probe of age-related changes in Bruch's membrane. This approach provides novel information on the range of AGE/ALE adducts and other associated biochemical groups occurring within this matrix during aging.…”

mentioning

confidence: 99%

Multiplex analysis of age-related protein and lipid modifications in human Bruch's membrane

Beattie

¹

,

Pawlak

²

,

Boulton

³

et al. 2010

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Aging of the human retina is characterized by progressive pathology, which can lead to vision loss. This progression is believed to involve reactive metabolic intermediates reacting with constituents of Bruch's membrane, significantly altering its physiochemical nature and function. We aimed to replace a myriad of techniques following these changes with one, Raman spectroscopy. We used multiplexed Raman spectroscopy to analyze the age-related changes in 7 proteins, 3 lipids, and 8 advanced glycation/lipoxidation endproducts (AGEs/ALEs) in 63 postmortem human donors. We provided an important database for Raman spectra from a broad range of AGEs and ALEs, each with a characteristic fingerprint. Many of these adducts were shown for the first time in human Bruch's membrane and are significantly associated with aging. The study also introduced the previously unreported up-regulation of heme during aging of Bruch's membrane, which is associated with AGE/ALE formation. Selection of donors ranged from ages 32 to 92 yr. We demonstrated that Raman spectroscopy can identify and quantify age-related changes in a single nondestructive measurement, with potential to measure age-related changes in vivo. We present the first directly recorded evidence of the key role of heme in AGE/ALE formation.

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“…Instrumental and methodological details have been published elsewhere (Cundy et al, 2015, Gamsjaeger et al, 2011a, Gamsjaeger et al, 2013, Gamsjaeger et al, 2010, Gamsjaeger et al, 2014a, Gamsjaeger et al, 2014b, Gamsjäger et al, 2009, Hofstetter et al, 2012). In the bone blocks, 600 individual measurements (each covering an area of ~ 1 × 1 μm) were obtained in randomly selected areas of interstitial bone, and the following Raman parameters calculated (Gamsjaeger et al, 2014b, Gamsjäger et al, 2009, Gamsjaeger et al, 2011b, Morris and Mandair, 2011): i) the mineral/matrix ratio (MM), ii) the relative proteoglycan content (PG), iii) the maturity/crystallinity (MMC) of the mineral crystallites, and iv) the relative content of two advanced glycation endproducts (AGEs), namely CML (ε- N -carboxymethyl- l -lysine) and PEN (Pentosidine) (Beattie et al, 2010, Beattie et al, 2011, Glenn et al, 2007, Pawlak et al, 2008). Pyrophosphate (PP) presence and spatial quantification in the bone tissue was determined using the Raman band around 360 cm − 1 (Chen et al, 2009, Cheng et al, 2009, Fuerst et al, 2010), normalized to mineral content (based on the v 2 PO 4 band), by RS imaging (210 × 130 μm areas) of open osteons.…”

Section: Methodsmentioning

confidence: 99%

Presence of pyrophosphate in bone from an atypical femoral fracture site: A case report

Shabestari

¹

,

Eriksen

²

,

Paschalis

³

et al. 2017

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Long-term antiresorptives use has been linked to atypical subtrochanteric and diaphyseal femoral fractures (AFF), the pathogenesis of which is still unknown. In the present case report we present the results of analysis of bone chips from a 74-year old female patient that had been on alendronate, ibandronate and denosumab treatment, and who sustained an atypical femoral fracture, by histology, quantitative backscattered electron imaging, and Raman spectroscopic analysis.The results indicate ongoing osteoclastic resorption, but also several abnormalities: 1) an altered arrangement of osteons; 2) impaired mineralization; 3) the presence of pyrophosphate, which might contribute to the impaired mineralization evident in the present case. Taken together, these changes may contribute to the focally reduced bone strength of this patient.

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“…Another theme in this context is the use of Raman spectroscopy to address medically related problems. Pawlak et al [174] reviewed results from a program designed to investigate the potential of Raman microscopy as a probe technique to address important problems in ophthalmology, looking at the disease age-related macular degeneration (AMD), the leading cause of irreversible blindness in the West.…”

Section: Raman Microscopymentioning

confidence: 99%

Instruments in Biotechnology and Medicine

Voss¹,

Feller²,

Beckmann³

2012

Handbook of Biophotonics

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The sections in this article are Photonic Instruments in Medicine Introduction Optical Window of Cells and Tissues Laser Light–Matter Interaction as a Function of Intensity Photochemical Treatment of Tissues Thermal Processes in Light–Matter Interaction Nonlinear Processes in Light–Matter Interaction (Not to Be Confused with Nonlinear Optics) Minimally Invasive Diagnostics – Endoscopy History Types and Components of Endoscopes Advantages and Disadvantages of Endoscopy Application Fields Combination of Standard White Light Endoscopic with Other Diagnostic Methods Some Trends in Endoscopy Noninvasive Diagnostics – Photoplethysmography Introduction Characterization of the PPG Signal Signal Acquisition Clinical Applications Summary Noninvasive Diagnostics – Ophthalmology Introduction Corneal Topography Perimetry Optical Biometry Retinal Imaging and Glaucoma Diagnostics Fluorescein Angiography Wavefront Analysis In Vitro Diagnostics – Microscopy Light Microscope Fluorescence Microscope New Fluorescence Microscope Techniques Confocal Microscopy Raman Microscopy In Vitro Diagnostics – Digital Slides and Virtual Microscopy In Vitro Diagnostics – Optical Methods in Clinical Analysis System Optical Spectroscopy of Blood and Urine Components Optical Spectroscopy of Tissues and Vessels Optical Spectroscopy of Agglutination and Precipitation – Nephelometry, Turbidimetry In Vitro Diagnostics – Blood Gas Pressure Measurements Optical Detection of p O 2 in Blood Optical Detection of p CO 2 in Blood Optical Detection of Further Monitoring Parameters in Intensive Care (Temperature, p H ) In Vitro Diagnostics – Photoacoustic Spectroscopy Therapy Introduction Laser Therapy in Ophthalmology – LASIK (Refraction Correction by Cornea Treatment) Photonic Instruments in Biotechnology Introduction Selected Analytical Methods Introduction Reflectometric Interference Spectroscopy ( RIf S ) Surface Plasmon Resonance ( SPR ) Fluorescence Methods Advanced Microscopic Techniques (Selection) Enzyme‐Linked Immunosorbent Assay ( ELISA ) Optical Biosensors, Biochips, Miniaturized Analytical Systems High‐Throughput Screening ( HTS ) Sample Preparation: Flow Injection Analysis Fermentation as a Central Process of Biotechnology General Aspects Fermentation Control Photobioreactors Optical Trap/Optical Tweezers Basic Scheme of Laser Tweezers and Micromanipulation Applications of Optical Tweezers

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Raman spectroscopy of advanced glycation end products (AGEs), possible markers for progressive retinal dysfunction

Cited by 26 publications

References 63 publications

Multiplex analysis of age-related protein and lipid modifications in human Bruch's membrane

Multiplex analysis of age-related protein and lipid modifications in human Bruch's membrane

Presence of pyrophosphate in bone from an atypical femoral fracture site: A case report

Instruments in Biotechnology and Medicine

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