Random Amplified Polymorphic DNA Analysis and Variation of Essential Oil Components of Atractylodes Plants.

Kohjyouma, Mareshige; Nakajima, Satoshi; Namera, Akira; Shimizu, Ryosuke; Mizukami, Hajime; Kohda, Hiroshi

doi:10.1248/bpb.20.502

Cited by 25 publications

(11 citation statements)

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“…Low expense, efficiency in developing large number of DNA markers in a short time, and requirement of basic molecular equipment has made the RAPD technique valuable although band reproducibility, problems of comigration, and scoring errors are a major problem. The technique has been applied in many plant groups such as Glycyrrhiza [42], Atractylodes [43,44], Astragalus [45], Amomun [46], Scutellaria [47], Panax [48][49][50][51], Aconitum [52], Ginkgo [53], Anectochilus [54], Lycium [55], Angelica [56], Bupleurum [57], Dendrobium [58], Magnolia [59], Asarum [60], Apocynum [61], Trollius [62], Phyllanthus [63], Indigofera [64], Coptis [65], Codonopsis [66], Taraxacum [67], Elephantopus [68], and Rehmannia [69]. AP-PCR (arbitrary polymerase chain reaction): AP-PCR (or arbitrarily chosen primers ACP-PCR) is a special case of RAPD using single primers approximately 10-50 bp in length [39].…”

Section: Pcr-based Methodsmentioning

confidence: 99%

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

Heubl¹

2010

Planta Med

136

104

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Section: Pcr-based Methodsmentioning

confidence: 99%

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

Heubl¹

2010

Planta Med

136

104

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“…Enzymatic Hydrolysis of 7 A mixture of 7 (16 mg) and b-glucosidase (5 mg; Toyobo Co. Ltd., lot 93240) in water (5 ml) was shaken in a water bath at 37°C for 30 d. The mixture was concentrated in vacuo to dryness and the residue was chromatographed over silica gel [CHCl 3 …”

Section: )mentioning

confidence: 99%

Glycosides of <i>Atractylodes lancea</i>

et al. 2003

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The rhizomes of Atractylodes plants (Compositae) have been used as an important crude drug since antiquity. They are listed in the Chinese, Korean, and Japanese pharmacopoeias, and are prescribed in traditional medicine as diuretic and stomachic drugs. They are classified into two groups that contain b-eudesmol and hinesol as the main constituents of the essential oil (Atractylodes lancea and Atractylodes chinensis; so-jutsu), and atractylon as the main constituent of the essential oil (Atractylodes japonica and Atractylodes ovata; byaku-jutsu).1) However, the phylogenetic relationship between A. lancea and A. japonica is considered to be closer than that of A. japonica and A. ovata. 2,3)As for the polar constituents of the rhizome of A. lancea, nine sesquiterpenoid glycosides (atractyloside A to I), L-tryptophan, and syringin were reported by Yahara et al. 4) On the other hand, we reported the isolation and the characterization of eight sesquiterpenoid glycosides, including atractyloside A-E, G, and a compound with a secoguaiane skeleton, a monoterpenoid glucoside, seven aromatic compound glycosides, and L-phenylalanine from the water-soluble portion of A. japonica. 5) In the present study, we undertook a reexamination of the water-soluble portion of the rhizome of A. lancea.The dried rhizome of A. lancea, which was cultivated in the Tokyo Metropolitan Medical Plants Garden, was extracted with methanol, and the methanolic extract was suspended in water and successively extracted with ether and ethyl acetate. The aqueous layer was chromatographed on Amberlite XAD-II to give water and methanol eluate fractions. The methanol eluate fraction was chromatographed on Sephadex LH-20, and subjected to a combination of silica gel, Lobar RP-8 column chromatography, and HPLC. Then, 16 sesquiterpenoid glycosides (1-16), four monoterpenoid glucosides (17-20), two hemiterpenoid glycosides (21, 22), an alkyl glycoside (23), five aromatic compound glycosides (24-28), an acetylene derivative compound glucoside (29), two nucleosides (30, 31), and L-tryptophan (32) were isolated. Among them, five sesquiterpenoid glycosides (7-9,

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“…Its rhizomes have been used for eliminating dampness, strengthening thespleen, expelling wind, and clearing away cold in traditional Chinese medicine for centuries (Qian et al, 2006). As for the chemical constituents of the rhizome of A. lancea, many sesquiterpenoid glycosides (Kitajima et al, 2003) and essential oil constituents (Takeda et al, 1996;Kohjyouma et al, 1997;Li et al, 2006) were reported. During our research of the phytochemical constituents of the aquous ethanol extract of the rhizomes of A. lancea, a new prenylated dihydrobenzofuran derivative and ten known compounds were isolated.…”

Section: Introductionmentioning

confidence: 99%

A new cytotoxic prenylated dihydrobenzofuran derivative and other chemical constituents from the rhizomes of Atractylodes lancea DC

Duan

Wang

Qian³

et al. 2008

Arch. Pharm. Res.

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A new prenylated dihydrobenzofuran derivative (1), was isolated from the rhizomes of Atractylodes lancea DC (Asteraceae), along with ten known compounds, including atractylenolide II (2), phi-taraxasteryl acetate (3), taraxerol acetate (4), beta-sitosterol (5), stigmasterol (6), beta-eudesmol (7), atractylenolide III (8), atractylenolide IV (9), daucosterol (10), and stigmasterol 3-O-beta-D-glucopyranoside (11). The structure of the new compound (1) was elucidated as trans-2-hydroxyisoxypropyl-3-hydroxy-7-isopentene-2,3-dihydrobenzofuran-5-carboxylic acid by the combination of 1D, 2D NMR analysis and mass spectrometry, and it was the first reported 2,3-dihydrobenzofuran derivative having a carboxyl residue at C-5 and an isopentene moiety at C-7 contemporaneously. In addition, compound 1 exhibited significant cytotoxicity against cancer cell lines HCT-116 and MKN-45.

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Random Amplified Polymorphic DNA Analysis and Variation of Essential Oil Components of Atractylodes Plants.

Cited by 25 publications

References 0 publications

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

Glycosides of <i>Atractylodes lancea</i>

A new cytotoxic prenylated dihydrobenzofuran derivative and other chemical constituents from the rhizomes of Atractylodes lancea DC

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