Random amplified polymorphic DNA analysis of salt-tolerant tobacco mutants generated by gamma radiation

Çelik, Özge; Atak, Çimen

doi:10.4238/2015.february.13.12

Cited by 4 publications

(5 citation statements)

References 24 publications

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“…Cross-breeding to breed salt-tolerant varieties has become a vital topic in the academic research of salt-tolerant plant breeding [3]. However, because of the long breeding cycle of kiwifruit, the genetic improvement process of cross-breeding is seriously hindered [4,5].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of the Salt-Treated Actinidia deliciosa (A. Chev.) C. F. Liang and A. R. Ferguson Plantlets

Zhuo

Zhao

et al. 2023

CIMB

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The area of saline land in the world is quite large, and there is broad room for its development and usage. ‘Xuxiang’ is an Actinidia deliciosa variety that is tolerant to salt and can be planted in an area of light-saline land, and has good comprehensive characteristics and high economic value. However, the molecular mechanism of salt tolerance is unknown at present. To understand the molecular mechanism of salt tolerance, the leaves of A. deliciosa ‘Xuxiang’ were used as explants to establish a sterile tissue culture system, and plantlets were obtained using this system. One percent concentration (w/v) of sodium chloride (NaCl) was employed to treat the young plantlets cultured in Murashige and Skoog (MS) medium, then RNA-seq was used for transcriptome analysis. The results showed that the genes related to salt stress in the phenylpropanoid biosynthesis pathway and the anabolism of trehalose and maltose pathways were up-regulated; however, those genes in the plant hormone signal transduction and metabolic pathways of starch, sucrose, glucose, and fructose were down-regulated after salt treatment. The expression levels of ten genes that were up-regulated and down-regulated in these pathways were confirmed by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. The salt tolerance of A. deliciosa might be related to the expression level changes in the genes in the pathways of plant hormone signal transduction, phenylpropanoid biosynthesis, and starch, sucrose, glucose, and fructose metabolism. The increased expression levels of the genes encoding alpha-trehalose-phosphate synthase, trehalose-phosphatase, alpha-amylase, beta-amylase, feruloyl-CoA 6-hydroxylase, ferulate 5-hydroxylase, and coniferyl-alcohol glucosyl transferase might be vital to the salt stress response of the young A. deliciosa plants.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of the Salt-Treated Actinidia deliciosa (A. Chev.) C. F. Liang and A. R. Ferguson Plantlets

Zhuo

Zhao

et al. 2023

CIMB

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“…Gamma radiation is well known to cause rapid and reliable physicochemical and biological alterations in organisms. [14][15][16] In mutation breeding studies, the next crucial step is to select the desired genotypes for new cultivars. Due to the fact that the selected mutants from M2 and M3 generations differ for several traits, it is important to discriminate the desired genotypes to improve new cultivars.…”

Section: Introductionmentioning

confidence: 99%

Raman Spectroscopic and Chemometric Investigation of Lipid–Protein Ratio Contents of Soybean Mutants

et al. 2019

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Seeds belonging to fourth generation mutants (M4) of Ataem-7 cultivar (A7) variety and S04-05 (S) breeding line salt-tolerant soybeans were investigated by Raman spectroscopy, complemented by chemometrics methods, in order to evaluate changes induced by mutations in the relative lipid–protein contents, and to find fast, efficient strategies for discrimination of the mutants and the control groups based on their Raman spectra. It was concluded that gamma irradiation caused an increase in the lipid to protein ratio of the studied Ataem-7 variety mutants, while it led to a decrease of this ratio in the investigated S04-05 breeding line mutants. These results were found to be in agreement with data obtained by reflectance spectrum analysis of the seeds in the full ultraviolet to near-infrared spectral region and suggest the possibility of developing strategies where gamma irradiation can be used as a tool to improve mutant soybean plants targeted to different applications, either enriched in proteins or in lipids. Ward's clustering and principal component analysis showed a clear discrimination between mutants and controls and, in the case of the studied S-type species, discrimination between the different mutants. The grouping scheme is also found to be in agreement with the compositional information extracted from the analysis of the lipid–protein contents of the different samples.

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“…They reported that 10 Gy gamma irradiation was found as the most effective dose to induce genetic variation in morphological traits, and they observed 100% polymorphism among the mutants. Gamma radiation-induced salt-tolerant oriental tobacco mutants were improved by Çelik and Atak [23]. Salt tolerance of the mutants was controlled by the callus induction in the presence of high salt concentration.…”

Section: Mutational Genomic Analysismentioning

confidence: 99%

“…RAPD is an efficient method to detect DNA alteration via using random primers. It has been started to use in earlier studies of genetic variabilities obtained by radiation treatments in Chrysanthemum [36,37], soybean [24], sugarcane, sunflower, groundnut [43], tobacco [23], potato [27], Rhododendron [25]. ISSR method is another molecular marker method widely used in plant biotechnology applications.…”

Section: Mutational Genomic Analysismentioning

confidence: 99%

“…Atak et al [24] used 100-500 Gy radiation doses produced by 137 Cs gamma source for soybean seeds, while Singh and Datta [29] used 60 Co gamma source at different doses ranging between 10 and 100 Gy for Triticum aestivum seeds. Ulukapı et al [21] also used 60 Co gamma source at 80-240 Gy radiation doses to induce genetic variability for Solanum melongena L. Çelik and Atak [23] used 100, 200, 300, and 400 Gy gamma rays by 137 Cs to determine the effective radiation dose for breeding studies of two Turkish tobacco varieties. They irradiated the tobacco seeds and selected the salt-tolerant mutants in M 3 progeny.…”

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confidence: 99%

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Applications of Ionizing Radiation in Mutation Breeding

Çelik¹,

Atak²

2017

New Insights on Gamma Rays

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As a predicted result of increasing population worldwide, improvements in the breeding strategies in agriculture are valued as mandatory. The natural resources are limited, and due to the natural disasters like sudden and severe abiotic stress factors, excessive floods, etc., the production capacities are changed per year. In contrast, the yield potential should be significantly increased to cope with this problem. Despite rich genetic diversity, manipulation of the cultivars through alternative techniques such as mutation breeding becomes important. Radiation is proven as an effective method as a unique method to increase the genetic variability of the species. Gamma radiation is the most preferred physical mutagen by plant breeders. Several mutant varieties have been successfully introduced into commercial production by this method. Combinational use of in vitro tissue culture and mutation breeding methods makes a significant contribution to improve new crops. Large populations and the target mutations can be easily screened and identified by new methods. Marker assisted selection and advanced techniques such as microarray, next generation sequencing methods to detect a specific mutant in a large population will help to the plant breeders to use ionizing radiation efficiently in breeding programs.

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Random amplified polymorphic DNA analysis of salt-tolerant tobacco mutants generated by gamma radiation

Cited by 4 publications

References 24 publications

Transcriptome Analysis of the Salt-Treated Actinidia deliciosa (A. Chev.) C. F. Liang and A. R. Ferguson Plantlets

Transcriptome Analysis of the Salt-Treated Actinidia deliciosa (A. Chev.) C. F. Liang and A. R. Ferguson Plantlets

Raman Spectroscopic and Chemometric Investigation of Lipid–Protein Ratio Contents of Soybean Mutants

Applications of Ionizing Radiation in Mutation Breeding

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