Random amplified polymorphic DNA analysis of eel genome

Jj, Qui

doi:10.1038/sj.cr.7290020

Cited by 4 publications

(2 citation statements)

References 6 publications

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“…However, more researches will also be necessary to identify the discrimination between/among populations and species, using an annealing control primer system. From what has been said above, the potential of this analysis to ascertain diagnostic markers for the identification of three eel species has also been verified ( Tasanakajon et al, 1998 ; Jing & Li, 1999 ; McCormack et al, 2000 ; Kimura et al, 2004 ; Kim et al, 2006 ; Yoon, 2008 ). It was reported that the species relation-ships revealed by the PCR approach should be consistent with previously obtained data using morphological portrait ( Nebauer et al, 2000 ).…”

Section: Resultsmentioning

confidence: 80%

“…As the eel culture industry grows, so doe’s interest into the genetics of this finfish species. However, in spite of their economic and scientific consequences, a little information currently exists regarding the genetic levels only of eel species in Korea and/or the other countries ( Min & Yang, 1993 ; Jing & Li, 1999 ; Kimura et al, 2004 ; Oh, 2011 ). In this study, to explicate the genetic distances and differences among geographical eel species, the author accomplished a clustering analysis of three eel species collected from the Yellow Sea.…”

Section: Introductionmentioning

confidence: 99%

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Differences and Variations among Anguilla japonica, Muraenesox cinereus and Conger myriaster from the Yellow Sea

Yoon

2015

Development &Amp; Reproduction

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Genomic DNAs were extracted from the muscle of twenty-one specimens of three eel species collected in Anguilla japonica (AJ), Muraenesox cinereus (MC) and Conger myriaster (CM) from the Yellow Sea, respectively. In the present study, 7 oligonucleotides primers generated 191 specific loci in the AJ species, 226 in the (MC) species and 181 in the CM species, respectively. The primer BION-02 generated the most loci (a total of 83), with an average of 11.86 in the AJ species. The specific loci generated by oligonucleotides primers exhibited inter-individual-specific characteristics, thus revealing DNA polymorphisms. With regard to average bandsharing value (BS) results, individuals from Conger myriaster species (0.808) exhibited higher bandsharing values than did individuals from Muraenesox cinereus species (0.729) (p<0.05). The longest genetic distance (0.430) displaying significant molecular difference was also between individual no. 01 within Anguilla japonica eel species and individual no. 04 within Anguilla japonica species. In this study, the dendrogram resulted from reliable seven oligonucleotides primers, indicating three genetic clusters composed of group I (ANGUILLA 01~ANGUILLA 07), group II (MURAENESOX 08~MURAENESOX 14) and group III (CONGER 15~CONGER 21). The existence of species differentiation and DNA polymorphisms among three eel species were detected by PCR analysis. As mentioned above, a dendrogram revealed close relationships between individual identities within three eel species. High levels of a significant genetic distance among three eel species showed this PCR approach is one of the most suitable tools for individuals and/or species biological DNA studies.

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Section: Resultsmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Differences and Variations among Anguilla japonica, Muraenesox cinereus and Conger myriaster from the Yellow Sea

Yoon

2015

Development &Amp; Reproduction

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Species composition of eels larvae (Anguillidae) in Mentawai Island waters, Indonesia based genetic data

Syaifullah

Sari

Tjong

et al. 2019

IOP Conf. Ser.: Earth Environ. Sci.

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The genetic variation of eels larvae Anguilla spp. which had migrated to the estuaries of North Pagai Island at Mentawai Islands was studied from September 2016 to January 2017. The objective of the present study was to validate the species composition of eels larvae that migrated to estuary of North Pagai Mentawai Islands, West Sumatra Province, Indonesia. The eels larvae were collected from Mabola, Simpungan, Saumanganya, and Taikako estuaries. A total of 10 samples from every location were used to analyse the genetic variation of eels. The result showed that the heterozygosities of Anguilla marmorata and A. bicolor pacifica are highest in Saumanganya estuary, 0.1863 and 0.1840, respectively, while A. bicolor bicolor is the highest in Taikako estuary (0.1518). Gene flow (Nm) values of eels A. marmorata, A. bicolor bicolor, and A. bicolor pacifica were 0.3656, 0.5309, and 0.4679, respectively The genetic differentiation value (Gst) of A. marmorata, A. bicolor bicolor, and A. bicolor pacifica were 0.8677, 0.4418, and 0.5686, respectively It is concluded that the genetic variation of inter-populations and intra-populations of A. marmorata was higher than A. bicolor bicolor and A. bicolor pacifica.

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Exequibilidade do DDRT-PCR para análise da expressão gênica diferencial em células de medula óssea murina

Almeida

Santos

Ribeiro-Paes

2012

Medicina (Ribeirão Preto)

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Modelo do estudo: Estudo Experimental. Introdução: Atualmente a pesquisa com células-tronco temgerado grande interesse devido a sua aplicabilidade no campo na medicina regenerativa. A medulaóssea é considerada a maior fonte de células-tronco adultas e o estabelecimento de novos métodospara a análise da expressão gênica torna-se estritamente necessário. Desse modo, o "DifferentialDisplay Reverse Transcription Polymerase Chain Reaction (DDRT-PCR)", pode ser uma ferramentaacessível para a investigação de pequenas diferenças no nível de expressão gênica em diferentes tiposcelulares, sob distintas condições.Objetivo: Neste presente trabalho nós investigamos a exequibilidade do DDRT-PCR na identificação dediferenças no nível de expressão gênica global em células da medula óssea de camundongos sobduas condições. Métodos: Primeiramente, a medula óssea foi isolada frescamente e uma secundaparte foi cultivada por uma semana sem troca de meio. Posteriormente, as células da medula (fresca ecultivada) foram submetidas a análise da expressão gênica, seguindo a metodologia de DDRT-PCR.Resultados: Inicialmente, foi possível identificar em células da medula óssea com uma semana decultivo, pequenas alterações morfológicas (células ovais para fibroblastóides) e no perfil de proteínas,por meio da visualização de bandas em SDS-Page gel. Finalmente, a análise da expressão gênica porDDRT-PCR, mostrou uma expressão diferencial com a presença de três bandas (1300, 1000 and 225pb) exclusivamente expressas na medula óssea fresca e mais duas bandas (400 and 300 pb) presentes somente nas células de medula cultivadas.Conclusões: Em suma, a metodologia de DDRT-PCR mostrou-se eficiente para a identificação depequenas diferenças no nível de expressão gênica em células da medula óssea sob duas definidascondições. Portanto, nós acreditamos que o DDRT-PCR possa ser designado de forma rápida e eficiente para a análise diferencial de expressão gênica em diferentes tipos de células-tronco, sob diferentescondições.

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Random amplified polymorphic DNA analysis of eel genome

Cited by 4 publications

References 6 publications

Differences and Variations among Anguilla japonica, Muraenesox cinereus and Conger myriaster from the Yellow Sea

Differences and Variations among Anguilla japonica, Muraenesox cinereus and Conger myriaster from the Yellow Sea

Species composition of eels larvae (Anguillidae) in Mentawai Island waters, Indonesia based genetic data

Exequibilidade do DDRT-PCR para análise da expressão gênica diferencial em células de medula óssea murina

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