An accurate estimation of forests’ aboveground biomass (AGB) is required because of its relevance to the carbon cycle, and because of its economic and ecological importance. The selection of appropriate variables from satellite information and physical variables is important for precise AGB prediction mapping. Because of the complex relationships for AGB prediction, non-parametric machine-learning techniques represent potentially useful techniques for AGB estimation, but their use and comparison in forest remote-sensing applications is still relatively limited. The objective of the present study was to evaluate the performance of automatic learning techniques, support vector regression (SVR) and random forest (RF), to predict the observed AGB (from 318 permanent sampling plots) from the Landsat 8 Landsat 8 Operational Land Imager (OLI) sensor, spectral indexes, texture indexes and physical variables the Sierra Madre Occidental in Mexico. The result showed that the best SVR model explained 80% of the total variance (root mean square error (RMSE) = 8.20 Mg ha−1). The variables that best predicted AGB, in order of importance, were the bands that belong to the region of red and near and middle infrared, and the average temperature. The results show that the SVR technique has a good potential for the estimation of the AGB and that the selection of the model hyperparameters has important implications for optimizing the goodness of fit.