Randomly amplified polymorphic DNA (RAPD) analysis of Atlantic Coast striped bass

Abstract

Atlantic Coast striped bass exhibit exceptionally low levels of genetic variation. The ability of the randomly amplified polymorphic DNA (RAPD) method to uncover genetic variation in this highly conserved species was investigated. Sufficient levels of variation were detected to allow a population genetic analysis of the four migratory populations of Atlantic Coast striped bass. Lynch's analogue of Wright's FST (F'ST) suggests that Atlantic Coast striped bass are genetically subdivided (F'ST for pooled Atlantic samples = 0.44). Significant heterogeneity was detected in the frequencies of 32 per cent of surveyed RAPD markers. A modification of Slatkin's conditional average frequency method suggests that gene flow is present among the sampled Atlantic Coast striped bass. Results of the RAPD analysis suggest that gene flow is sufficient to prevent fixation of alternate genetic markers, but not sufficient to prevent the development of significant divergence in frequencies of these markers.

Phylogenetic relationships among Spanish Barbus species (Pisces, Cyprinidae) shown by RAPD markers

“…The higher polymorphism of some populations, such as CIJ, GUA and MAN, are due mainly to the presence of a high number of bands in low frequencies (Table 2). In general, levels of polymorphism are low compared with those seen in other fish RAPD studies (Bielawski and Pumo, 1997;Caccone et al, 1997;Gomes et al, 1998;Mamuris et al, 1999). Table 3 shows the similarity values within-(on the diagonal) and between-populations (above the diagonal).…”

“…There is now increasing evidence that the RAPD technique, which has been used in different fields, can detect nuclear variation in fish (Borowski et al, 1995;Naish et al, 1995;Sultmann et al, 1995;Bielawski and Pumo, 1997;Caccone et al, 1997;Callejas and Ochando, 1998;Mamuris et al, 1999;Allendorf and Seeb, 2000). These studies have shown that RAPD is an extremely sensitive method for detecting DNA variation and for establishing genetic relationships in closely related organisms.…”

RAPD markers indicate the occurrence of structured populations in a migratory freshwater fish species

“…The potential use of RAPD in genetic mapping and population genetics has been widely documented for a large variety of organisms, including fish (Postlethwait et al, 1994;Foo et al, 1995;Bielawski and Pumo, 1997;Caccone et al, 1997;Dergam et al, 1998;Nadig et al, 1998;Liu et al, 1999).…”

Genetic variability in four fish species (Pimelodus maculatus, Prochilodus lineatus, Salminus brasiliensis and Steindachneridion scripta) from Uruguay River basin

“…Informations derived from molecular genetic techniques shall contribute significantly for the preservation of aquatic genetic resources and sustainable development (Carvalho, 1993;Chiari, 1999;Martins et al, 2003;Almeida et al, 2003). The RAPD procedure (Randomly Amplified Polymorphic DNA) has been instrumental to understand the genetic variability of fish populations (Bielawski and Pumo, 1997;Caccone et al, 1997;Callejas and Ochando, 1998;Dergam et al, 1998;Nadig et al, 1998;Liu et al, 1999;Prioli et al, 2002;Shikano and Taniguchi, 2002;Almeida et al, 2003;Bártfai et al, 2003;Hatanaka and Galetti Jr, 2003). This technique consists of amplification by PCR (Polymerase Chain Reaction) of random segments of genomic DNA using a single short primer of arbitrary sequence.…”