Rapamycin promotes Schwann cell migration and nerve growth factor secretion

Liu, Fang; Zhang, Haiwei; Zhang, Kaiming; Wang, Xinyu; Li, Shipu; Yin, Yi

doi:10.4103/1673-5374.130101

Cited by 15 publications

(2 citation statements)

References 58 publications

(60 reference statements)

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“…As is well known, cell movement and migration, together with cell growth, are vital for organ development and post-developmental tissue formation. Previous studies have shown that enhanced Schwann cell migration would promote nerve regeneration [ 22 , 23 ]. The results in the current study suggest that at the acute stage, cells started response to stimuli induced by sciatic nerve crush and migrated toward external signals for encouraging wound healing and regeneration of injured nerves.…”

Section: Discussionmentioning

confidence: 99%

Deep Sequencing and Bioinformatic Analysis of Lesioned Sciatic Nerves after Crush Injury

et al. 2015

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The peripheral nerve system has an intrinsic regenerative capacity in response to traumatic injury. To better understand the molecular events occurring after peripheral nerve injury, in the current study, a rat model of sciatic nerve crush injury was used. Injured nerves harvested at 0, 1, 4, 7, and 14 days post injury were subjected to deep RNA sequencing for examining global gene expression changes. According to the temporally differential expression patterns of a huge number of genes, 3 distinct phases were defined within the post-injury period of 14 days: the acute, sub-acute, and post-acute stages. Each stage showed its own characteristics of gene expression, which were associated with different categories of diseases and biological functions and canonical pathways. Ingenuity pathway analysis revealed that genes involved in inflammation and immune response were significantly up-regulated in the acute phase, and genes involved in cellular movement, development, and morphology were up-regulated in the sub-acute stage, while the up-regulated genes in the post-acute phase were mainly involved in lipid metabolism, cytoskeleton reorganization, and nerve regeneration. All the data obtained in the current study may help to elucidate the molecular mechanisms underlying peripheral nerve regeneration from the perspective of gene regulation, and to identify potential therapeutic targets for the treatment of peripheral nerve injury.

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Section: Discussionmentioning

confidence: 99%

Deep Sequencing and Bioinformatic Analysis of Lesioned Sciatic Nerves after Crush Injury

et al. 2015

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“…When distal site of injured nerve witnessed Waller degeneration, SCs began to proliferate and then participated in swallowing degeneration of axons and myelin debris forming a longitudinal continuous cell cord (B ǜ ngner's band), which guided the growth of regenerating axons. SCs are essential for regeneration of microenvironment [ 3 ], which is the important condition for neuronal survival and neurite growth. SCs have nutrition effects on neuron due to synthetic neurotrophic factors, and they also contribute to generating neurite growth factors [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Angelica Extract on Schwann Cell Proliferation and Expressions of Related Proteins

Jiang

Liu

Zhang

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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The present study investigated the effects of Angelica extract (AE) on Schwann cell proliferation and expressions of related proteins, including brain derived neurotrophic factor (BDNF), neural cell adhesion molecule (NCAM), and proliferating cell nuclear antigen (PCNA). Proliferation activity and cell cycles of SCs were evaluated by MTT assay and flow cytometry methods, respectively, after 12 h treatment of AE at different concentrations (62.5, 125, 250, 1000, 2000, 4000, and 8000 mg/L). SCs were treated by 500, 1000, and 2000 mg/L AE for 24 h or 48 h; the related genes mRNA and proteins expressions in SCs were detected by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) kit. At the concentration range of 125–2000 mg/L, the SC proliferation was induced by AE in a dose-dependent manner, especially 1000 and 2000 mg/L; cells in drug-treated groups showed the most increase. Cells counts were ascended significantly in (G2/M + S) phase compared to control group. BDNF, NCAM, and PCNA protein expressions significantly increased at drug-treated groups. Relative genes mRNA expressions levels were also significantly higher compared to control group. The results indicated that AE facilitated SC proliferation and related genes and proteins expressions, which provided a basic guideline for nerve injury repair in clinic.

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Combinatorial tissue engineering partially restores function after spinal cord injury

Hakim

Rodysill

Chen

et al. 2019

J Tissue Eng Regen Med

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Hydrogel scaffolds provide a beneficial microenvironment in transected rat spinal cord. A combinatorial biomaterials‐based strategy provided a microenvironment that facilitated regeneration while reducing foreign body reaction to the three‐dimensional spinal cord construct. We used poly lactic‐co‐glycolic acid microspheres to provide sustained release of rapamycin from Schwann cell (SC)‐loaded, positively charged oligo‐polyethylene glycol fumarate scaffolds. The biological activity and dose‐release characteristics of rapamycin from microspheres alone and from microspheres embedded in the scaffold were determined in vitro. Three dose formulations of rapamycin were compared with controls in 53 rats. We observed a dose‐dependent reduction in the fibrotic reaction to the scaffold and improved functional recovery over 6 weeks. Recovery was replicated in a second cohort of 28 animals that included retransection injury. Immunohistochemical and stereological analysis demonstrated that blood vessel number, surface area, vessel diameter, basement membrane collagen, and microvessel phenotype within the regenerated tissue was dependent on the presence of SCs and rapamycin. TRITC‐dextran injection demonstrated enhanced perfusion into scaffold channels. Rapamycin also increased the number of descending regenerated axons, as assessed by Fast Blue retrograde axonal tracing. These results demonstrate that normalization of the neovasculature was associated with enhanced axonal regeneration and improved function after spinal cord transection.

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Rapamycin promotes Schwann cell migration and nerve growth factor secretion

Cited by 15 publications

References 58 publications

Deep Sequencing and Bioinformatic Analysis of Lesioned Sciatic Nerves after Crush Injury

Deep Sequencing and Bioinformatic Analysis of Lesioned Sciatic Nerves after Crush Injury

Effects of Angelica Extract on Schwann Cell Proliferation and Expressions of Related Proteins

Combinatorial tissue engineering partially restores function after spinal cord injury

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