RAPD analysis of somaclonal variants derived from embryo callus cultures of peach

Hashmi, Ghazala; Huettel, R. N.; Meyer, Rhonda C.; Krusberg, L. R.; Hammerschlag, F. A.

doi:10.1007/bf01275503

Cited by 96 publications

(26 citation statements)

References 25 publications

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“…Since then, somaclonal variation in in vitro derived sugarcane has been consistently observed, particularly when plants are produced via a callus stage, which involves exposure to high levels of auxin (3-5 mg/L 2,4-D) and long culture periods (Abo-Elwafa, 2004;Snyman et al, 2011). Hashmi et al (1997), analyzed somaclonal variants derived from peach embryonic callus culture, and found 29% polymorphic fragments (Cavusoglu and Sulusoglu, 2013). Shoyama et al (1997) studied regenerated plantlets via direct somatic embryogenesis of Panax notoginseng and found evidence of genetic homogeneity when compared with the original plants.…”

Section: Resultsmentioning

confidence: 99%

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Hsie¹,

Brito²,

Nova³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Sugarcane culture is an important source of income for the Brazilian economy. The aim of this study was to identify somaclonal variation in sugarcane varieties RB943365 and RB92579 arising from micropropagation using inter-simple sequence repeat (ISSR) DNA markers. The evaluated plants were generated from the in vitro propagation of shoot tips grown in MS medium supplemented with vitamins, myoinositol, glycine, and sucrose, without the use of growth regulators. Fifteen consecutive subcultures with intervals of 14 days were carried out, and DNA was extracted from young leaves obtained from each of the subcultures. The DNA was amplified with ISSR markers and separated by electrophoresis on 2% agarose gels. No evidence of polymorphism was observed in subcultures of the varieties analyzed, suggesting the absence of somaclonal variants. In this way, the ISSR marker was efficient at analyzing somaclonal variation, and in vitro propagation of sugarcane can be considered efficient for 15 consecutive subcultures of the varieties analyzed.

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Section: Resultsmentioning

confidence: 99%

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Hsie¹,

Brito²,

Nova³

et al. 2015

Genet. Mol. Res.

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“…Similar results were reported using the RAPD technique in Norway spruce (Fourré et al 1997), Japanese black pine (Goto et al 1998), Japanese white oak (Thakur et al 1999), loblolly pine (Tang 2001), almond (Martins et al 2004), hazelnut (Nas et al 2004, pedunculate oak (Valladares et al 2006), cork oak (Fernandes et al 2011), apricot (Soliman 2012), annatto (Siril and Joseph 2013), and seedless lemon (Goswami et al 2013). In contrast, RAPD analysis reported genetic variation in poplar (Ostry et al 1994;Rani et al 1995), white spruce (Isabel et al 1995(Isabel et al , 1996, peach (Hashmi et al 1997), teak (Gangopadhyay et al 2003), Idaho locust (Ngezahayo et al 2006), stone pine (Cuesta et al 2010), and olive (Leva et al 2012).…”

Section: Discussionmentioning

confidence: 53%

Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.) and field performance of somatic embryo-derived trees

Hosoi

Kuramoto

Maruyama

2015

Plant Biotechnology

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In this study, 100 random amplified polymorphic DNA (RAPD) primers were screened to assess clonal fidelity in somatic embryos of Sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.). Of the 100 primers tested, eight were selected based on their amplification products, which showed clear DNA fragmentation, generating 8.75 bands per primer in average. The amplification products showed no genetic variation among the tested somatic embryos. In addition, the performance of somatic embryo-derived trees was monitored in the field, and their mean height growth was measured until the age of 10 years. Although no phenotypic differences were observed, the somatic embryo-derived trees showed lower growth compared with seedlings.

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“…Thus, random amplified polymorphic DNA (RAPD) markers have been used in peach [31] cedar [32], lemon [33] and chestnut [34], whereas amplified fragment length polymorphism (AFLP) has been used in kiwifruit [35], grape [36] and apple [37]. In the last few years, microsatellites have been widely used to analyze many plant species, including arabian coffee [38], cork oak [39] and trembling aspen [40].…”

Section: Discussionmentioning

confidence: 99%

Haploids in Conifer Species: Characterization and Chromosomal Integrity of a Maritime Pine Cell Line

Cabezas¹,

Morcillo

Vélez³

et al. 2016

Forests

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Abstract:Haploids are a valuable tool for genomic studies in higher plants, especially those with huge genome size and long juvenile periods, such as conifers. In these species, megagametophyte cultures have been widely used to obtain haploid callus and somatic embryogenic lines. One of the main problems associated with tissue culture is the potential genetic instability of the regenerants. Because of this, chromosomal stability of the callus and/or somatic embryos should also be assessed. To this end, chromosome counting, flow cytometry and genotyping using microsatellites have been reported. Here, we present an overview of the work done in conifers, with special emphasis on the production of a haploid cell line in maritime pine (Pinus pinaster L.) and the use of a set of molecular markers, which includes Single Nucleotide Polymorphisms (SNPs) and microsatellites or Single Sequence Repeats (SSRs), to validate chromosomal integrity confirming the presence of all chromosomic arms.

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RAPD analysis of somaclonal variants derived from embryo callus cultures of peach

Cited by 96 publications

References 25 publications

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.) and field performance of somatic embryo-derived trees

Haploids in Conifer Species: Characterization and Chromosomal Integrity of a Maritime Pine Cell Line

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