RAPD and ISSR analyses of regenerated pea Pisum sativum l. plants

Кузнецова, О. И.; Ash, O. A.; Hartina, G. A.; Gostimskij, S. A.

doi:10.1007/s11177-005-0009-9

Cited by 9 publications

(11 citation statements)

References 29 publications

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“…two of 5 superior somaclones showing two fold increase in total oil content showed gross changes in RAPD banding pattern. Kuznetsova et al (8) analysed regenerants of long-term callus cultures of different pea genotypes (R-9, W-1 and cultivar Viola). Regenerants displayed changes both in qualitative and in quantitative traits.…”

Section: Resultsmentioning

confidence: 99%

“…it involves amplification of genomic DNA with short and random primers at low annealing temperatures (22). this technique is employed by many researchers in genetic screening of plant tissue cultures and regenerants (8,12). cReD-RA "coupled Restriction Enzyme Digestion-Random Amplification" is a method to detect DnA methylation, which is one of the main causes of epigenetic variations (2).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic and Epigenetic Variations in Barley Calli Cultures

Temel

Kartal

Gözükırmızı

2008

Biotechnology & Biotechnological Equipment

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic and Epigenetic Variations in Barley Calli Cultures

Temel

Kartal

Gözükırmızı

2008

Biotechnology & Biotechnological Equipment

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“…Previously, the RAPD technique has been applied to assess genetic diversity in pea (Simioniuc et al 2002), green gram (Karuppanapandian et al 2006a), cow pea (Karuppanapandian et al 2006b), and black gram (Karuppanapandian et al 2007). The ISSR technique has been used to examine genetic relationships in genus Vigna (Souframanien and Gopalakrishna 2004) and several other crops (Awasthi et al 2004;Kuras et al 2004;Kuznetsova et al 2005;Thimmappaiah et al 2009). In black gram varieties, ISSR markers amplified polymorphic bands more efficiently than RAPD markers (77.8 compared with 66.5%, respectively; Table 3) and similar results have been obtained for several other crops, including wheat (Nagoaka and Ogihara 1997) and groundnut (Raina (Table 3).…”

Section: Rapd and Issr Comparisonmentioning

confidence: 99%

Efficiency of RAPD and ISSR markers in assessing genetic diversity and relationships in black gram (Vigna mungo L. Hepper) vari

Karuppanapandian

Wang²,

Karuppudurai

et al. 2010

Can. J. Plant Sci.

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, W. 2010. Efficiency of RAPD and ISSR markers in assessing genetic diversity and relationships in black gram (Vigna mungo L. Hepper) varieties. Can. J. Plant Sci. 90: 443Á452. The DNA fingerprinting methodologies, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were used to estimate genetic diversity and relationships among 20 black gram (Vigna mungo L. Hepper) varieties. Thirty selected RAPD primers amplified 255 bands, 168 of which were polymorphic (66.5%). On average, these primers produced 8.5 bands, 5.6 of which were polymorphic. Polymorphic band number varied from 2 (A-05) to 10 (OPA-02), with sizes ranging from 100 to 2550 bp. Twenty-four selected ISSR primers produced 238 amplified products, 184 of which were polymorphic (77.8%). On average, these primers generated 9.8 bands, with 7.7 polymorphic bands ranging in number from 4 (ISSR-13) to 11 (ISSR-03), and size from 100Á2650 bp. Genetic relationships were estimated using similarity coefficient (Jaccard's) values between different accession pairs; these varied from 30.7 to 85.0 for RAPD, and from 37.2 to 88.4 with ISSR. UPGMA analysis indicated that the varieties ranged in similarity from 0.50 to 1.00 (mean of 0.75) for RAPD, and from 0.47 to 1.00 (mean of 0.76) with ISSR. Cluster analysis of RAPD and ISSR results identified three clusters with significant bootstrap values, which revealed greater homology between the varieties. Principal coordinates analysis also supported this conclusion. Among the black gram varieties, WBU-108 and RBU-38 were highly divergent, whereas LBG-648 and LBG-623 were genetically similar. The markers generated by RAPD and ISSR assays can provide practical information for the management of genetic resources and these results will also provide useful information for the molecular classification and breeding of new black gram varieties.

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“…Moreover, Kuznetsova et al (2005) showed their reliability in analysing DNA polymorphisms generated by long-term culture and subsequent regeneration in pea. In our study, the same molecular markers were also shown to be efficient in determining the genetic changes induced by tissue culture and by artificial DNA demethylation.…”

Section: Discussionmentioning

confidence: 99%

Somaclonal variation at the nucleotide sequence level in rice (Oryza sativa L.) as revealed by RAPD and ISSR markers, and by pairwise sequence analysis

Ngezahayo

Yu²,

2007

J Appl Genet

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The nature of somaclonal variation at the nucleotide sequence level was studied in rice cv. Nipponbare. First, we investigated genomic variations by using 2 molecular marker systems: RAPD (random amplified polymorphic DNA) and ISSR (inter-simple sequence repeat). This was followed by sequencing of selected bands that represented genomic variations, and pairwise sequence analysis taking advantage of the whole genome sequence of rice. In addition, transpositional activity of the active MITE, mPing, was analysed by locus-specific PCR amplifications. The 2-year-old calli and their regenerated plants, analysed with 24 RAPD and 20 ISSR primers, showed moderate levels of genomic variation (20.83% and 17.04%, respectively). To test whether DNA methylation plays a role in somaclonal variation, the calli were treated with 5-azacytidine, a chemical agent that reduces cytosine methylation by blocking the activity of DNA methyltransferase. Though dwarfism occurred in regenerants from treated calli (a hallmark of the drug treatment), there was only a slight increase in the frequency of somaclonal variation detected in the treated calli and their regenerated plants relative to untreated controls. The transposon mPing also remained immobile in both treated and untreated calli. Nevertheless, dendrograms constructed according to the Jaccard coefficient calculated by UPGMA of the ISSR bands revealed that the 5-azacytidine-treated and untreated somaclones were grouped into 2 distinct clusters, suggesting a possible indirect effect of the treatment on the genomic changes, depending on the marker used. Sequence analysis indicated a low level of variation (0.31%), with single-base-pair substitutions predominating.

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RAPD and ISSR analyses of regenerated pea Pisum sativum l. plants

Cited by 9 publications

References 29 publications

Genetic and Epigenetic Variations in Barley Calli Cultures

Genetic and Epigenetic Variations in Barley Calli Cultures

Efficiency of RAPD and ISSR markers in assessing genetic diversity and relationships in black gram (Vigna mungo L. Hepper) vari

Somaclonal variation at the nucleotide sequence level in rice (Oryza sativa L.) as revealed by RAPD and ISSR markers, and by pairwise sequence analysis

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