RAPD markers linked with restorer genes for the C‐source of cytoplasmic male sterility in rye (Secale cereale L.)

Abstract

The study was aimed at the identification of random amplified polymorphic DNA markers linked to genes controlling male sterility in rye with the C-source of sterility-inducing cytoplasm. Markers of male sterility were distinguished using bulk segregant analysis, carried out on the two F 2 crosses between male sterile and male fertile inbred lines. Screening of polymorphisms revealed by 1000 arbitrary 10-mer primers allowed the detection of 10 markers in the cross between 711-cmsC and DS2 lines and seven markers in the cross between 544-cmsC and Ot0-20 lines. Five markers were common for the two crosses, which allowed comparative mapping to be performed. Ten markers were mapped on the 4RL chromosome arm where two linked quantitative trait loci (QTL) for male sterility were discovered. Additional QTL of minor effect on male fertility were detected between the two linked markers provisionally assigned to the 6RS chromosome arm. The effectiveness of the marker-assisted selection (MAS) for male-sterile genotypes was evaluated.

The importance of chromosomes from the sixth homeologic group in the restoration of male fertility in winter triticale with Triticum timopheevii cytoplasm

“…Quantitative trait loci (QTL) analysis allowed for the localisation of additional less efficient restorers on chromosomes 2A, 4B and 6A (Ahmed et al 2001). In rye (the second ancestor of the triticale), QTL analyses performed on F 2 mapping populations resulted in the detection of genes restoring male fertility in CMS systems on chromosomes 1R, 3R, 4R, 5R and 6R (Miedaner et al 2000; Stojałowski et al 2004). …”

“…The significance of genotype by environment interactions for the detection of QTL controlling different traits is usually investigated by the assessment of “immortal” mapping populations (recombinant inbred lines, DH lines) or by the verification of results from F 2 in the F 3 generation. Unfortunately, research on male sterility restoration is frequently limited to the one generation (Miedaner et al 2000; Stojałowski et al 2004) because the reproduction of male sterile genotypes by self-pollination is impossible. Application of the partial F 3 generation obtained from only fertile plants can be recommended for the identification of heterozygous F 2 plants, which is reasonable for investigations on one effective restorer gene (Börner et al 1998; Hackauf et al 2012).…”

The application of GBS markers for extending the dense genetic map of rye (Secale cereale L.) and the localization of the Rfc1 gene restoring male fertility in plants with the C source of sterility-inducing cytoplasm

“…The source material for this population was a hybrid between male-sterile inbred line 544C containing the C cytoplasm and the restorer inbred line Ot0-20. Research conducted on the F 2 generation of this hybrid indicated that the paternal line contained at least two effective restorer genes and that one of the supposed genes was located on the 4RL chromosome (Stojałowski et al 2004 ). The male-fertile hybrid plants from the F 1 and following generations were used for consecutive back-crossing with male-sterile line 544C.…”

“…Nuclear genes that restore male fertility in rye with Pampa and Vavilovii cytoplasms are spread throughout the rye genome; rough mapping analyses have indicated that they are localized on chromosomes 1R, 3R, 4R, 5R, and 6R (Börner et al 1998 ; Miedaner et al 2000 ; Bednarek et al 2002 ; Stojałowski et al 2004 ). Among these genes, those most efficient at fertility restoration were located on the long arm of chromosome 4R.…”

Inheritance of resistance to angular leaf spot (Pseudomonas syringae pv. lachrymans) in cucumber and identification of molecular markers linked to resistance

“…The PCR amplifications were performed using a PTC‐200 thermocycler (MJ Research) as follows: initial denaturation at 95°C for 1 min, followed by 10 cycles of denaturation at 94°C for 5 s, annealing at 37°C for 30 s and elongation at 72°C for 30 s, followed by 35 cycles at temperatures: 94°C for 5 s, 37°C for 30 s and 72°C for 1 min. The final extension was performed for 7 min at 72°C (Stojalowski et al ., 2004). Amplicons were detected by electrophoresis on a 1·5% agarose gel with 0·5 × TBE buffer, stained with ethidium bromide and photographed using a digital camera and a UV trans‐illuminator.…”