RAPD, SCAR and conserved 18S rDNA markers for a red-listed and endemic medicinal plant species, Knema andamanica (Myristicaceae)

Sheeja, T. E.; Anju, P. R.; Shalini, R.; Siju, S.; Dhanya, K.; Krishnamoorthy, B

doi:10.1007/s12298-013-0166-6

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…In conservation biology, differentiating between closely related species with the use of molecular markers is considered important for biodiversity studies (Agarwal et al 2008;Sheeja et al 2013). The null alleles, which are present only in heterozygous genotypes, cannot be detected from dominant RAPD markers, and RAPD variation cannot be identified as adaptive genes (Bekessy et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity of Saraca asoca (Roxb.) De Wilde: a commercially important, but endangered, forest tree species in Western Ghats, India

Saini

Hegde

et al. 2018

N.Z. j. of For. Sci.

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Background: Saraca asoca (Roxb.) De Wilde is a valuable tree used in traditional medicine against a variety of ailments. Almost all parts of the tree are used for various commercial herbal preparations. Due to overexploitation, the species is rapidly disappearing from Western Ghats where it is native and grew extensively until recently. Conservation of this important medicinal plant is therefore an urgent need. To plan effective conservation strategies, a scientific assessment of the genetic diversity and distribution is needed. Methods: Random Amplified Polymorphic DNA fingerprinting was employed and the population genetic structure of seven wild and three cultivated populations totalling 160 individuals of S. asoca in the Western Ghats region of Karnataka, Maharashtra and Goa for analysis. Results: Variation of 89% was observed within populations while 11% was observed among the populations of S. asoca. Gene flow (Nm) of 2.01 was observed, and 0.19 was the degree of genetic differentiation recorded. Unweighted Pair Group Method with Arithmetic average (UPGMA) cluster analysis generated a dendrogram that showed an admixture of all genotypes but with two major clusters, which was also supported by a STRUCTURE-based Bayesian model. One wild population was well, but inexplicably, differentiated from the rest. Conclusions: The study shows that there is still considerable genetic diversity existing in natural populations of S. asoca, suggesting good natural cross-pollination, giving encouraging indications that the gene pool is under no immediate threat. Any conservation strategy should utilise the observed genetic variation in the choice of planting stock for programmes of conservation, propagation and reforestation.

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Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity of Saraca asoca (Roxb.) De Wilde: a commercially important, but endangered, forest tree species in Western Ghats, India

Saini

Hegde

et al. 2018

N.Z. j. of For. Sci.

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“…The RAPD markers produced distinct banding patterns, hence allowing successful species discrimination (Arif et al, 2010b). Similarly, Sheeja et al (2013) used RAPD markers for the discrimination of Knema andamanica from neighboring taxa in Qatar. The authors used clustering analysis to separate K. andamanica from other closely related taxa.…”

Section: Standard Molecular and Genomic Techniques In Plant Biodiversmentioning

confidence: 99%

“…This technique successfully identified K. andamanica from the most genetically related species. The authors referred to the SCAR technique as a robust methodology and suggested its use as a DNA barcode marker in species authentication (Sheeja et al, 2013).…”

Section: Standard Molecular and Genomic Techniques In Plant Biodiversmentioning

confidence: 99%

The Promise of Molecular and Genomic Techniques for Biodiversity Research and DNA Barcoding of the Arabian Peninsula Flora

et al. 2019

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The Arabian Peninsula is known to have a comprehensive and rich endowment of unique and genetically diverse plant genetic resources. Analysis and conservation of biological diversity is a crucial issue to the whole Arabian Peninsula. The rapid and accurate delimitation and identification of a species is crucial to genetic diversity analysis and the first critical step in the assessment of distribution, population abundance and threats related to a particular target species. During the last two decades, classical strategies of evaluating genetic variability, such as morphology and physiology, have been greatly complemented by phylogenetic, taxonomic, genetic diversity and breeding research molecular studies. At present, initiatives are taking place around the world to generate DNA barcode libraries for vascular plant flora and to make these data available in order to better understand, conserve and utilize biodiversity. The number of herbarium collection-based plant evolutionary genetics and genomics studies being conducted has been increasing worldwide. The herbaria provide a rich resource of already preserved and identified material, and these as well as freshly collected samples from the wild can be used for creating a reference DNA barcode library for the vascular plant flora of a region. This review discusses the main molecular and genomic techniques used in plant identification and biodiversity analysis. Hence, we highlight studies emphasizing various molecular techniques undertaken during the last 10 years to study the plant biodiversity of the Arabian Peninsula. Special emphasis on the role of DNA barcoding as a powerful tool for plant biodiversity analysis is provided, along with the crucial role of herbaria in creating a DNA barcode library.

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“…Only two reports have been published so far, which discussed the presence of various phytochemicals like tannins, terpenes, flavonoids, steroids, etc., in some other species of the genus Knema (Salleh and Ahmad 2017;Supriya and Sreekanth 2021). The absence of proper knowledge and documentation of the genus Knema and over-exploitation by the local people marked many species of the genus Knema, including Knema linifolia, as "decreasing" species in the IUCN red list of threatened species (Sheeja et al 2013). Only a few K. linifolia trees have been reported alive in India, mainly in Assam, Meghalaya, Alipurduar & Darjeeling districts of West Bengal (Barstow and Timberlake 2018).…”

Section: Introductionmentioning

confidence: 99%

Analyzing the antimicrobial efficacy of the economically important tree Knema linifolia (Roxb.) Warb

Bhar,

Das,

Haldar

et al. 2024

J Exp Bio & Ag Sci

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Knema linifolia is widely used for fuel wood, fodder and healthcare purposes. This plant treats various diseases in different parts of India, including Assam, Meghalaya, Alipurduar and Darjeeling districts of West Bengal. This study was carried out to determine the bactericidal properties of various parts of K. linifolia aqueous extract. The aqueous extract of the leaves, bark, stem and plant sap were tested against Escherichia coli (gram-negative bacteria) & Staphylococcus aureus (gram-positive bacteria). Among the tested extracts, both the leaf and bark extracts were found to have high bactericidal potential and can kill more than 60% of both bacterial strains with a concentration of 300µg/mL through an agar diffusion test. The MIC (Minimum Inhibitory Concentration) values for the leaf and bark extracts were recorded at ≤1000µg/mL & ≤500µg/mL, respectively. It has also been found that both the bark and leaf extracts contain high tannins, which might be essential for the antibacterial properties of Knema sp. There is currently a lack of proper documentation on using K. linifolia, which makes it challenging to conduct clinical or commercial research to support new uses in modern phototherapy. This study aims to fill this gap and provide significant information that could lead to changes in modern medicine.

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RAPD, SCAR and conserved 18S rDNA markers for a red-listed and endemic medicinal plant species, Knema andamanica (Myristicaceae)

Cited by 7 publications

References 22 publications

Assessment of genetic diversity of Saraca asoca (Roxb.) De Wilde: a commercially important, but endangered, forest tree species in Western Ghats, India

Assessment of genetic diversity of Saraca asoca (Roxb.) De Wilde: a commercially important, but endangered, forest tree species in Western Ghats, India

The Promise of Molecular and Genomic Techniques for Biodiversity Research and DNA Barcoding of the Arabian Peninsula Flora

Analyzing the antimicrobial efficacy of the economically important tree Knema linifolia (Roxb.) Warb

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