RAPD variation within and among natural populations of outcrossing willow-leaved foxglove (Digitalis obscura L.)

Nebauer, Sergio G.; Castillo-Agudo, Lucas del; Segura, Juan

doi:10.1007/s001220051159

Cited by 65 publications

(35 citation statements)

References 31 publications

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“…The RAPD technique has been widely used both for studies on wild plants (Yeh et al 1995;Khasa and Dancik 1996;Owuor 1997;Fornari et al 1999;Nebauer et al 1999) and for studies on cultivated plants (Sharma et al 1995;Divaret et al 1999;Moeller et al 1999). By contrast researches employing the ISSR technique have mainly focused on cultivated species (Moreno et al 1998;Wang et al 1998;Blair et al 1999).…”

Section: Discussionmentioning

confidence: 99%

“…RAPD and ISSR markers have been used both for DNA fingerprinting (Moreno et al 1998;Blair et al 1999;Divaret et al 1999;Gilbert et al 1999), for population genetic studies (Wolfe et al 1998;Nebauer et al 1999) and for phylogenetic studies (Hess et al 2000). There are also some reports in which the capacity of RAPD and ISSR to detect polymorphism and to discriminate among taxa has been compared (Nagaoka and Ogihara 1997;Esselman et al 1999).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of ISSR and RAPD markers to characterize three Chilean Nothofagus species

Mattioni

Casasoli

González³

et al. 2002

Theor Appl Genet

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The present study is the first report of fingerprinting on three Chilean Nothofagus species using ISSR and RAPD markers; 61 Nothofagus nervosa, 32 Nothofagus obliqua and 32 Nothafagus dombeyi individual trees, sampled from collection and natural sites, were analyzed. Among 45 primers tested, the 6 ISSR and 6 RAPD primers selected for the analysis generated a total of 63 ISSR and 42 RAPD fragments. A high proportion of polymorphic bands, ranging from 97% and 80%, was found using both markers. A similar number of private and marker bands was generated by both markers in all the species examined and one discriminant ISSR fragment was obtained for N. dombeyi. Jaccard and Dice similarity indices were used to evaluate pairwise genetic divergence; cluster analysis of the similarity matrices was performed to estimate the intra- and inter-specific genetic diversity, and PCA analysis was employed to evaluate the resolving power of the markers to differentiate between the species. These analyses, carried out for both markers, allowed us to identify three main groups corresponding to the three Nothofagus species. The results of the present study can be seen as a starting point for future researches on the population and evolutionary genetics of these species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of ISSR and RAPD markers to characterize three Chilean Nothofagus species

Mattioni

Casasoli

González³

et al. 2002

Theor Appl Genet

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“…The RAPD technique has been successfully used in a variety of taxonomic and genetic diversity studies (Jain et al 1994;Li et al 1999;McGrath et al 1999;Nebauer et al 1999;Rodriguez et al 1999), and was found by Wolf et al (1999) and by us to be suitable for use with Echinacea species in its ability to reproducibly generate polymorphic markers. As this study was being completed, Wolf et al (1999) published a report demonstrating the utility of the RAPD technique for the discrimination of E. purpurea, E. angustifolia, and E. pallida using two different primers.…”

Section: Discussionmentioning

confidence: 99%

Genetic relationships and diversity of commercially relevant Echinacea species

2002

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The genus Echinacea is comprised of nine species, which are perennial herbs indigenous to North America and which have been traditionally used as medicinal plants for centuries. Three Echinacea species, E. angustifolia, E. purpurea, and E. pallida, are currently being traded internationally in the natural products market. Echinacea products constitute a significant portion of this growing, multi-billion dollar industry. The increasing popularity of Echinacea products has led to the expansion of wildcrafting and commercial cultivation to meet the growing demand for plant material. Echinacea is considered of value as a nonspecific immune stimulant, and claims of its efficacy have been tentatively supported by both laboratory and clinical studies. This study used random amplified polymorphic DNA (RAPD) markers to determine the genetic relationships of the three Echinacea species of commercial interest, to evaluate the level of diversity present within germplasm of each of the three species, and to compare accessions of each species available from different sources. A total of 101 RAPD markers were generated for the 76 individuals of four species included in the analysis. NTSYS-pc was used to evaluate the genetic relationships of the three species and to determine the general level of overall diversity. Analysis of molecular variance (AMOVA) was performed using pruned marker sets corrected for the dominant nature of RAPD markers. AMOVA revealed that most of the variation occurred within accessions of the same species, though some accessions of both E. pallida and E. angustifolia were found to be significantly different from other accessions of the same species.

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“…Random amplified polymorphic DNA (RAPD) technology (Williams et al, 1990) is a means of investigating genetic diversity within and between populations and has been applied to many plants (Nebauer et al, 1999(Nebauer et al, , 2000Ester et al, 2001;Jover et al, 2003). Once established, RAPD-PCR (polymerase chain reaction) has the advantage of being quick and easy, requiring little plant material, and having a high resolution (Gugerli et al, 1999;Bronzini et al, 2002).…”

Section: Introductionmentioning

confidence: 97%

RAPD Profiling in Detecting Genetic Variation in Endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China

et al. 2005

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Random amplified polymorphic DNA (RAPD) markers were used to measure genetic diversity of Coelonema draboides (Brassicaceae), a genus endemic to the Qilian Mountains of the Qinghai-Tibet Plateau. We sampled 90 individuals in 30 populations of Coelonema draboides from Datong and Huzhu counties of Qinghai Province in P.R. China. A total of 186 amplified bands were scored from the 14 RAPD primers, with a mean of 13.3 amplified bands per primer, and 87% (161 bands) polymorphic bands (PPB) was found. Analysis of molecular variance (AMOVA) shows that a large proportion of genetic variation (84.2%) resides among individuals within populations, while only 15.8% resides among populations. The species shows higher genetic diversity between individuals than other endemic and endangered plants. The RAPDs provide a useful tool for assessing genetic diversity of rare, endemic species and for resolving relationships among populations. The results show that the genetic diversity of this species is high, possibly allowing it to adapt more easily to environmental variations. The main factor responsible for the high level of differentiation within populations and the low level of diversity among populations is probably the outcrossing and long-lived nature of this species. Some long-distance dispersal, even among far separated populations, is also a crucial determinant for the pattern of genetic variation in the species. This distributive pattern of genetic variation of C. draboides populations provides important baseline data for conservation and collection strategies for the species. It is suggested that only populations in different habitats should be studied and protected, not all populations, so as to retain as much genetic diversity as possible.

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RAPD variation within and among natural populations of outcrossing willow-leaved foxglove (Digitalis obscura L.)

Cited by 65 publications

References 31 publications

Comparison of ISSR and RAPD markers to characterize three Chilean Nothofagus species

Comparison of ISSR and RAPD markers to characterize three Chilean Nothofagus species

Genetic relationships and diversity of commercially relevant Echinacea species

RAPD Profiling in Detecting Genetic Variation in Endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China

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