Rapeseed Oil and Ginseng Saponins Work Synergistically To Enhance Th1 and Th2 Immune Responses Induced by the Foot-and-Mouth Disease Vaccine

Zhang, Cenrong; Wang, Yuemin; Wang, Meng; Su, Xiaoyan; Lu, Yisong; Su, Fei; Hu, Songhua

doi:10.1128/cvi.00127-14

Cited by 23 publications

(16 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously demonstrated the combined adjuvant effects of mineral oil or vegetable oil supplemented by ginseng saponins (GS) [11][12][13][14]. The present study showed that injection of an FMD vaccine emulsified in SO containing GS and VE (SO-VE-GS) in mice induced significantly higher IgG, IgG1, and IgG2a than those containing GS or VE alone.…”

Section: Discussionmentioning

confidence: 54%

“…immunized twice with 0.2 mL of FMDV antigen in saline solution or emulsified in SO-VE-GS or ISA 206 at a 2-week interval. Sera was collected 7 days before and 3, 7,14,21,28,35, and 42 days after the booster immunization to detect FMDV-specific IgG. Then the mice were euthanized to collect bone marrow cells for analysis of antibody-secreting bone marrow plasma cells (BMPCs).…”

Section: Immunization and Sample Collectionmentioning

confidence: 99%

See 1 more Smart Citation

Early IgG Response to Foot and Mouth Disease Vaccine Formulated with a Vegetable Oil Adjuvant

et al. 2019

Self Cite

View full text Add to dashboard Cite

The present study evaluated soybean oil (SO) containing vitamin E (VE) and ginseng saponins (GS) (SO-VE-GS) for their adjuvant effect on foot-and-mouth disease (FMD) vaccine. Since mineral oil ISA 206 is a common adjuvant used in the FMD vaccine, it was used as a control adjuvant in this study. VE and GS were found to have a synergistic adjuvant effect. When mice were immunized with the FMD vaccine emulsified in SO with VE and GS, significantly higher serum IgG, IgG1, and IgG2a were found than VE and GS used alone. SO-VE-GS and ISA 206 behaved differently in adjuvant activities. When mice were immunized with the FMD vaccine adjuvanted with SO-VE-GS, significantly higher and earlier production of serum IgG was found than that adjuvanted with ISA 206. Although both adjuvants significantly increased the number of bone marrow plasma cells, a stimulation index of lymphocytes (SI) as well as the production of IL-4 and IL-6, SO-VE-GS promoted significantly higher SI and the ratio of CD4+/CD8+ T cells with production of increased IFN-γ and decreased TGF-β1 as compared with the ISA 206 group. The data suggested that SO-VE-GS activated Th1/Th2 immune responses. Transcriptome analysis of splenocytes showed that differentially expressed genes (DEGs), immune-related gene ontology (GO) terms, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were significantly enriched in the SO-VE-GS group. Therefore, the potent adjuvant effect of SO-VE-GS on the FMD vaccine may be attributed to the immune-related gene profile expressed in lymphocytes. Due to its plant origin and due to being much cheaper than imported mineral oil ISA 206, SO-VE-GS deserves further study in relation to vaccines used in food animals.

show abstract

Section: Discussionmentioning

confidence: 54%

Section: Immunization and Sample Collectionmentioning

confidence: 99%

Early IgG Response to Foot and Mouth Disease Vaccine Formulated with a Vegetable Oil Adjuvant

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Combining adjuvants could be as beneficial as the tailoring of the response, and several publications have shown synergistic effects. Plant‐derived adjuvants, in this case rapeseed oil and ginseng saponins (Zhang et al., ), and the synthetic TLR7/8 ligand R848 and poly I:C combined with a traditional aluminium hydroxide‐adjuvanted vaccine (Zhou et al., ), have shown promise. An oil/gel combination (ISA 201, Carbigen, Emulsigen‐D) also induced improved immunity compared to traditional vaccines in pigs.…”

Section: Literature Reviewmentioning

confidence: 99%

Global Foot-and-Mouth Disease Research Update and Gap Analysis: 3 - Vaccines

Robinson

Knight-Jones

Charleston

et al. 2016

Transbound Emerg Dis

View full text Add to dashboard Cite

SummaryThis study assessed research knowledge gaps in the field of FMDV (foot-andmouth disease virus) vaccines. The study took the form of a literature review combined with research updates collected in 2014 from 33 institutes from across the world. Findings were used to identify priority areas for future FMD vaccine research. Vaccines play a vital role in FMD control, used both to limit the spread of the virus during epidemics in FMD-free countries and as the mainstay of disease management in endemic regions, particularly where sanitary controls are difficult to apply. Improvements in the performance or cost-effectiveness of FMD vaccines will allow more widespread and efficient disease control. FMD vaccines have changed little in recent decades, typically produced by inactivation of whole virus, the quantity and stability of the intact viral capsids in the final preparation being key for immunogenicity. However, these are exciting times and several promising novel FMD vaccine candidates have recently been developed. This includes the first FMD vaccine licensed for manufacture and use in the USA; this adenovirus-vectored FMD vaccine causes in vivo expression of viral capsids in vaccinated animals. Another promising vaccine candidate comprises stabilized empty FMDV capsids produced in vitro in a baculovirus expression system. Recombinant technologies are also being developed to improve otherwise conventionally produced inactivated vaccines, for example, by creating a chimeric vaccine virus to increase capsid stability and by inserting sequences into the vaccine virus for desired antigen expression. Other important areas of ongoing research include enhanced adjuvants, vaccine quality control procedures and predicting vaccine protection from immune correlates, thus reducing dependency on animal challenge studies. Globally, the degree of independent vaccine evaluation is highly variable, and this is essential for vaccine quality. Previously neglected, the importance of evaluating vaccination programme effectiveness and impact is increasingly being recognized.

show abstract

“…According to the findings of Yu et al, GSLS given orally to immunosuppressed chickens before immunization restores splenocyte proliferation, increases the number of intestinal immune cells and boosts HI antibody titers to a bivalent vaccine against Newcastle disease (ND) and avian influenza (AI). When supplemented with rapeseed oil, an oil adjuvant or oil‐emulsified FMD vaccine, GSLS reportedly shows a synergistic adjuvant effect . Rivera et al have found that addition of ginseng saponins to a bivalent vaccine of porcine parvovirus and Erysipelothrix rhusiopathiae results in production of significantly higher antibody titers in pigs .…”

Section: Introductionmentioning

confidence: 99%

“…When supplemented with rapeseed oil, an oil adjuvant or oil-emulsified FMD vaccine, GSLS reportedly shows a synergistic adjuvant effect. [10][11][12] Rivera et al have found that addition of ginseng saponins to a bivalent vaccine of porcine parvovirus and Erysipelothrix rhusiopathiae results in production of significantly higher antibody titers in pigs. 13 Further, selenium (Se), an essential micronutrient and trace element, has been shown to play significant roles in immunity and antioxidant defense.…”

mentioning

confidence: 99%

Ginseng stem‐leaf saponins in combination with selenium enhance immune responses to an attenuated pseudorabies virus vaccine

Maqbool

Wang

Cui

et al. 2019

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

Pseudorabies, a herpesvirus infection, is mainly controlled by using attenuated live vaccines. In this study, the effect of ginseng stem and leaf saponins (GSLS) in combination with selenium (Se; in the form of sodium selenite) on vaccination against attenuated pseudorabies virus (aPrV) was evaluated. It was found that GSLS and Se have an adjuvant effect and that a combination of GSLS and Se stimulates significantly enhanced immune responses than does GSLS or Se alone. Following oral administration of GSLS, mice immunized with an attenuated PrV vaccine diluted in Se‐containing physiological saline solution (PSS) provoked a significantly stronger gB‐specific serum antibodies response (IgG, IgG1 and IgG2a), enhanced lymphocyte proliferation and cytolytic activity of NK cells, along with higher production of cytokines (IFN‐γ, IL‐12, IL‐5 and IL‐10) by splenocytes. Notably, the combination of GSLS and Se conferred a much higher resistance to fPrV challenge after immunization of the mice with aPrV vaccine. This study offers convincing experimental evidence that an injection of Se with oral GSLS is a promising adjuvant combination that improves the efficacy of vaccination against PrV and deserves further study regarding improvement of responses to other animal vaccines.

show abstract

Rapeseed Oil and Ginseng Saponins Work Synergistically To Enhance Th1 and Th2 Immune Responses Induced by the Foot-and-Mouth Disease Vaccine

Cited by 23 publications

References 27 publications

Early IgG Response to Foot and Mouth Disease Vaccine Formulated with a Vegetable Oil Adjuvant

Early IgG Response to Foot and Mouth Disease Vaccine Formulated with a Vegetable Oil Adjuvant

Global Foot-and-Mouth Disease Research Update and Gap Analysis: 3 - Vaccines

Ginseng stem‐leaf saponins in combination with selenium enhance immune responses to an attenuated pseudorabies virus vaccine

Contact Info

Product

Resources

About