Rapid action of oestradiol against hydrogen peroxide-induced oxidative stress in cataractous lens epithelium: an in vitro study

Gajjar, Devarshi; Patel, Dhara; Alapure, Bhagwat V.; Praveen, Mamidipudi R.; Johar, Kaid; Vasavada, Abhay R.

doi:10.1038/eye.2008.284

Cited by 9 publications

(6 citation statements)

References 34 publications

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“…Gottipati and Cammarata reported that 17 b-estradiol protects human lens epithelial cells against oxidative stress via increasing the activity of mitochondrial superoxide dismutase [30]. A recent study by Gajjar et al demonstrated the protective effect of estradiol against hydrogen peroxide-induced oxidative stress in lens epithelium cells [31].…”

Section: Discussionmentioning

confidence: 97%

Effect of estrogen replacement therapy on lens epithelial cell apoptosis in an experimental rat model

et al. 2009

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Epidemiologic studies have revealed a higher incidence of cataracts in estrogen-deprived postmenopausal women, although the pathogenic mechanism has not yet been elucidated. Apoptosis of lens epithelial cells has been associated with cataractogenesis. The aim of the study reported here was to investigate the effect of estrogen replacement therapy (ERT) on lens epithelial cell apoptosis in an experimental rat model. Forty female Wistar rats were randomized into four groups: ERT (17beta-estradiol, 10 microg/kg/day) for 3 months without ovariectomy (group 1) and with ovariectomy (group 2); only ovariectomy (group 3); sham operated (group 4). At the end of the third month, all rats were sacrificed in estrous cycle, as determined by the vaginal smear test, and their right eyes were enucleated. Enucleated eyes were analyzed by immunohistochemical methods for the expression of terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end (TUNEL), caspase-3, and bcl-2 labeling. The TUNEL, caspase-3, and bcl-2 staining scores were found to increase in group 3 rats following the ovariectomy compared to the sham-operated group. The ERT decreased these scores in rats with or without the ovariectomy; however, these differences were not statistically significant. These data suggest that estrogen does not significantly affect lens epithelial cell apoptosis. Further studies are needed to gain a better understanding of the protective mechanism of estrogen and to provide new ideas for the treatment and prevention of cataract.

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Section: Discussionmentioning

confidence: 97%

Effect of estrogen replacement therapy on lens epithelial cell apoptosis in an experimental rat model

et al. 2009

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“…Several studies have supported the intracellular and intercellular signaling function of H 2 O 2 [14,15]. The synthesis of catalase in LECs was reported to be dependent on the concentration of H 2 O 2 [16]. It was also reported that H 2 O 2 activates the expression of glutathione peroxidase, an inactivator of H 2 O 2 [17].…”

Section: Discussionmentioning

confidence: 99%

Immunolocation of Aquaporin 8 in Human Cataractous Lenticular Epithelial Cells

Hayashi

Fukuda

et al. 2017

Biomed Hub

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Purpose/Aim: Aquaporin 8 (AQP8) is a diffusion facilitator of hydrogen peroxide (H₂O₂) through cell membranes. The purpose of this study was to confirm and localize AQP8 in human lenticular epithelial cells (LECs). Materials and Methods: Lenticular anterior capsule samples, including LECs, were collected during cataract surgery of cataract patients after informed consent. The localization of AQP8 was detected by immunohistochemical staining using an antibody to AQP8. Real-time polymerase chain reaction (RT-PCR) was also used to determine the AQP8 mRNA expression levels. The PCR products were analyzed by gel electrophoresis following analyses of band density. Results: Immunohistochemical staining showed AQP8 was distributed throughout the whole area of the anterior capsulotomy. AQP8 labeling was observed surrounding and within the cytoplasm of LECs. RT-PCR and gel electrophoresis also revealed the presence of AQP8 mRNA in the lenticular anterior capsule. The results of immunohistochemical staining were comparable to those of RT-PCR and gel electrophoresis. Conclusions: The results of this study indicate the distribution of AQP8 in human LECs. This is the first investigation confirming the presence of AQP8 in human LECs.

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“…Once the removal anterior capsule is removed, the sample was instantly kept in an Essential Medium (containing 10% fetal bovine serum) and immediately transferred to the Research laboratory. A single rhexis for preservation was kept in Minimal Essential Medium, containing 10% fetal bovine serum, and incubated in an incubator containing 5% carbon dioxide at 37°C [11]. The maximum time-lapse from the collection of the sample to the process was 15-20 min.…”

Section: Detailed Examination Of Senile Cataract Patientsmentioning

confidence: 99%

Dna Damage in Lens Epithelial Cells of Senile Cataract Patients of Different Prakriti According to Ayurveda Literature

Jadhao

Gawali

Lokhande

et al. 2023

Asian J Pharm Clin Res

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Objectives: The Ayurvedic concept of the constitution is useful in predicting an individual’s susceptibility to age-related diseases like Cataracts (Kaphaja Linganasha). The objectives of the study were to assess DNA damage directly in human lens epithelial cells (HLEC) of senile cataracts of Vata Predominant, Pitta Predominant, and Kapha Predominant Prakriti individuals. Methods: After obtaining Institutional Ethics Committee permission, HLEC were taken from 20 Vatta Predominant,20 Pitta Predominant and 20 Kapha Predominant Prakriti individuals of cataract after cataract surgery and from 4 controls in which quantitative assessment of DNA damage were measured using CometScore™ software. The formation of “comets” in the DNA of lens epithelial cells can be visualized through the method of single gel electrophoresis and indicates DNA strand breaks, as the damaged DNA migrates at a different rate than non-damaged DNA during electrophoresis. Results: No such prominent comets were indicating any DNA damage in the HLEC of the four control subjects, but comets were found in cataractous HLEC. The maximal damage was found in pitta-predominant Prakriti Individuals. In senile cataract patients, in HLECs DNA was randomly damaged and this type of damage was possible by reactive oxygen species. The DNA damage in HLEC was found maximally in pitta Predominant Prakriti individuals of senile type of cataract patients. Statistical significance was observed between senile cataracts in pitta predominant Prakriti versus senile cataracts in Vata predominant Prakriti individuals and between senile cataracts in Vata predominant Prakriti versus senile cataracts in Kapha Prakriti individual. No statistically significant results were obtained for senile cataracts in pitta Prakriti versus senile cataracts in Kapha Prakriti individuals. Conclusion: The pathogenesis of senile cataracts is multifactorial and includes continuous molecular stress brought by photo-oxidative stress, UV irradiation, and oxidative reactions.

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Rapid action of oestradiol against hydrogen peroxide-induced oxidative stress in cataractous lens epithelium: an in vitro study

Cited by 9 publications

References 34 publications

Effect of estrogen replacement therapy on lens epithelial cell apoptosis in an experimental rat model

Effect of estrogen replacement therapy on lens epithelial cell apoptosis in an experimental rat model

Immunolocation of Aquaporin 8 in Human Cataractous Lenticular Epithelial Cells

Dna Damage in Lens Epithelial Cells of Senile Cataract Patients of Different Prakriti According to Ayurveda Literature

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