Rapid adhesion and proliferation of keratinocytes on the gold colloid/chitosan film scaffold

Functional enhancement of chitosan and nanoparticles in cell culture, tissue engineering, and pharmaceutical applications

“…In 2009, they found that the gold colloid/chitosan scaffold could promote adhesion and proliferation of keratinocytes (Zhang et al, 2009; Lu et al, 2010). Commercialization of these new technologies is sprouting almost as fast as they are developed, such as some of the wound dressing materials associated with nanoparticles (Rustogi et al, 2005; Ulkur et al, 2005).…”

Alginate–chitosan/hydroxyapatite polyelectrolyte complex porous scaffolds: Preparation and characterization

“…Synthetic polymers including polycaprolactone [3], poly(ethylene oxide) [4] and poly(vinyl alcohol) [5] are appealing as scaffold materials because they can be produced with specific properties such as molecular weights, block structures and cross-linking modes. Naturally derived polymers such as alginate [6], chitosan [7], collagen [8], fibrin [9], gelatin [10] and hyaluronate [11] are of special interest due to their biological and chemical similarities to natural tissues. There are many methods to manufacture porous scaffolds including porogen leaching, emulsion freeze-drying, 3D printing, phase separation techniques and so forth.…”

Novel Chitin and Chitosan Materials in Wound Dressing

“…These results demonstrate that the smad3 ASOs-polyelectrolyte complex composed of chitosan and sodium alginate scaffold could be applied for accelerated wound healing (Hong et al, 2008). The gold colloid/chitosan film scaffold, accelerate proliferation of newborn mice keratinocytes, was fabricated by self-assembly technology (Zhang et al, 2009). In this study, the keratinocytes were cultured and observed on three different extracellular matrices: gold colloid/chitosan film scaffold, chitosan film and cell culture plastic (control groups).…”

“…The rapidly proliferating keratinocytes were indentified and characterized by immunohisto chemistry and TEM, which showed the cells maintain their biological activity well. The results indicated that gold colloid/chitosan film scaffold was nontoxic to keratinocytes, and was a good candidate for wound dressing in skin tissue engineering (Zhang et al, 2009). Recently, a few studies have been reported on chitin scaffolds with Ag nanoparticles to treat patients with deep burns, wounds etc.…”