Rapid Aggregation of Gold Nanoparticles Induced by Non-Cross-Linking DNA Hybridization

Sato, Kae; Hosokawa, Kazuo; Maeda, Mizuo

doi:10.1021/ja034876s

Cited by 658 publications

(530 citation statements)

References 14 publications

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“…UV/visible spectra showed a red shift which was related with an increase in size and aggregation of nanoparticles or combination of both (Sato et al 2003) (Fig. 1).…”

Section: Effect Of the Initial Ph On Synthesis Of Aunpsmentioning

confidence: 97%

Synthesis and study of catalytic application of l-methionine protected gold nanoparticles

et al. 2017

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Gold nanoparticle is growing class of nanotechnology due to large number of uses. We synthesized stable L-methionine protected gold nanoparticles (AuNps) by in situ reduction of HAuCl 4 using sodium borohydrate as reducing and L-methionine as stabilizing agent in an aqueous medium. Different parameters (pH, capping agent, precursor salt, and heating time) were optimized to see the effect on the size of particles. Double beam spectrophotometer was used to carry out the spectroscopic studies. It was observed that pH and concentration of reducing salt are deciding factors in controlling the size and morphology of AuNps. Scanning electron microscopy (SEM) verified the formation of AuNPs as predicted by UV-Vis spectra. The interaction of AuNPs with L-methionine was confirmed by Fourier Transform Infrared (FTIR). The reduction of 4-nitrophenol acted as standard of reaction to check the response of AuNps catalyst. Complete reduction of 4-nitrophenol was accomplished by AuNps sol in just 60 s. Fastest reduction rate was observed with smaller spherical particles. This study concluded that size and shape of AuNps can be monitored by controlling the pH, concentration of capping and reducing agent. It also provides an economical solution to aquatic environment in terms of time saving and use of small volume of catalytic solution for reduction of several other toxic organic pollutants.

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“…UV/visible spectra showed a red shift which was related with an increase in size and aggregation of nanoparticles or combination of both (Sato et al 2003) (Fig. 1).…”

Section: Effect Of the Initial Ph On Synthesis Of Aunpsmentioning

confidence: 97%

Synthesis and study of catalytic application of l-methionine protected gold nanoparticles

et al. 2017

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“…In fact, AuNPs functionalized with ssDNA capable of specifically hybridizing to a complementary target for the detection of specific nucleic acid sequences in biological samples have been extensively used (Li and Rothberg, 2004;Mirkin et al, 1996;Cheng et al, 2006;Baptista et al, 2008;Doria et al, 2007b;Taton et al, 2000;Qin and Yung, 2007;Sato et al, 2005;Sato et al, 2003;Elghanian et al, 1997).…”

Section: Nanodiagnosticsmentioning

confidence: 99%

Multifunctional Gold Nanocarriers for Cancer Theranostics: From Bench to Bedside and Back Again?

Conde

Tian²,

Baptista

et al. 2014

Nano-Oncologicals

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After a quarter of century of rapid technological advances, research has revealed the complexity of cancer, a disease intimately related to the dynamic transformation of the genome. However, the full understanding of the molecular onset of this disease is still far from achieved and the search for mechanisms of treatment will follow closely.It is here that Nanotechnology enters the fray offering a wealth of tools to diagnose and treat cancer.It is indisputable that the use of gold nanocarriers has been gaining momentum as vectors for therapy and diagnostic strategies, combining the AuNPs' ease of functionalization with numerous biomolecules, high loading capacity and fast uptake by target cells. In fact, over the last decade nearly 12.000 research papers focusing on multifunctional gold nanocarriers have been published in peer-reviewed journals. Some of the described nanosystems will most likely revolutionize our understanding of biological mechanisms and push forward the clinical practice through their 2 integration in future diagnostics platforms. Nevertheless, very little gave fruitful results in order to improve a bench-to-bedside approach to translational research. On the basis of theoretical and experimental results obtained so far are we or not at the point: from bench to bedside and back again? As you will see, the answers to this question are complex, but one thing is clear: Translation into clinics is a tortuous and difficult path. Here, we provide a critical review about the available multifunctional gold nanocarriers for in vitro application and in vivo cancer targeting on nanodiagnostics and therapy.

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“…aggregation) [6,7]. Examples of the use of this property include the recognition of a specific DNA strand [8,9] as well as protein-ligand systems such as (strept)avidinbiotin [10,11].…”

Section: Introductionmentioning

confidence: 99%

Biotinylation of optically responsive gold/polyelectrolyte nanostructures

2014

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The development of analytical platforms incorporating Au nanoparticles (NPs) for optical biosensing requires approaches to produce optically responsive and target selective materials. In the present work, fluorescence emission and bioselectivity of Au NPs have been combined by a one-step surface functionalization using fluorescein isothiocyanate (FITC) and biotin. First, colloidal Au NPs (d=17.7±2.7 nm) exhibiting an absorption maximum (λ max ) at 524.5 nm have been surface-modified with the polyelectrolytes (PE) poly(allylamine hydrochloride) (PAH) and poly(sodium styrene sulfonate) (PSS). Two strategies were then assessed for the functionalization of Au NPs: (i) functionalization of PAH with biotin and subsequent coating of the NPs and (ii) surface modification of the NPs with the PE followed by postfunctionalization with biotin. The effect of the surface functionalization on the colloidal NPs was monitored by optical absorption and fluorescence emission spectroscopies, dynamic light scattering (DLS), zeta potential measurements and transmission electron microscopy (TEM). Finally, the optical response and specificity of the functionalized NPs towards avidin was evaluated by optical measurements. The results have shown that these hybrid nanostructures have potential to be used in biosensing. In particular, the aggregation of biotinylated Au assemblies associated with the biorecognition of avidin could be assessed by fluorescence emission spectroscopy.

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Rapid Aggregation of Gold Nanoparticles Induced by Non-Cross-Linking DNA Hybridization

Cited by 658 publications

References 14 publications

Synthesis and study of catalytic application of l-methionine protected gold nanoparticles

Synthesis and study of catalytic application of l-methionine protected gold nanoparticles

Multifunctional Gold Nanocarriers for Cancer Theranostics: From Bench to Bedside and Back Again?

Biotinylation of optically responsive gold/polyelectrolyte nanostructures

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