Rapid analysis of hypolipidemic drugs in a live zebrafish assay

Zhou, Juan; Xu, Yiqiao; Guo, Sheng-Ya; Li, Chunqi

doi:10.1016/j.vascn.2014.12.002

Cited by 47 publications

(33 citation statements)

References 29 publications

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“…21 The zebrafish facility at Hunter Biotechnology, Inc. is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International. 22 Chemicals and drugs PHZ (lot #: G1324044) and O-dianisidine (lot #: 119-90-4) were purchased from Aladdin company of Shanghai, China. Aspirin and clopidogrel were bought from Sigma-Aldrich, diltiazem hydrochloride injection was from Mitsubishi Tanabe Pharma Corporation, xuanshuantong injection was from Wuzhou Pharmaceutical Corporation, salvianolate injection was from Shanghai Green Valley Pharmaceutical, and astragalus injection was from Chiatai Qingchunbao.…”

Section: Zebrafish Care and Maintenancementioning

confidence: 99%

A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs

Zhu

Liu²,

Guo³

et al. 2016

Zebrafish

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Thrombosis is a leading cause of death and the development of effective and safe therapeutic agents for thrombotic diseases has been proven challenging. In this study, taking advantage of the transparency of larval zebrafish, we developed a larval zebrafish thrombosis model for drug screening and efficacy assessment. Zebrafish at 2 dpf (days post fertilization) were treated with phenylhydrazine (PHZ) and a testing drug for 24 h. Tested drugs were administered into the zebrafish either by direct soaking or circulation microinjection. Antithrombotic efficacy was quantitatively evaluated based on our previously patented technology characterized as an image analysis of the heart red blood cells stained with O-dianisidine staining. Zebrafish at 2 dpf treated with PHZ at a concentration of 1.5 μM for a time period of 24 h were determined as the optimum conditions for the zebrafish thrombosis model development. Induced thrombosis in zebrafish was visually confirmed under a dissecting stereomicroscope and quantified by the image assay. All 6 human antithrombotic drugs (aspirin, clopidogrel, diltiazem hydrochloride injection, xuanshuantong injection, salvianolate injection, and astragalus injection) showed significant preventive and therapeutic effects on zebrafish thrombosis (p < 0.05, p < 0.01, & p < 0.001) in this zebrafish thrombosis model. The larval zebrafish thrombosis model developed and validated in this study could be used for in vivo thrombosis studies and for rapid screening and efficacy assessment of antithrombotic drugs.

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Section: Zebrafish Care and Maintenancementioning

confidence: 99%

A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs

Zhu

Liu²,

Guo³

et al. 2016

Zebrafish

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“…11,13,14 In addition, zebrash has been reported to be an excellent model for lipid metabolism, and a useful animal model for the analysis of anti-obesity effects of natural products in a shorter time compared with rodents. [14][15][16][17] Previous researches, which studied the effect of high-fat diet on the body fat accumulation in zebrash, concluded that zebrash is a suitable animal model in research into obesity induced by a diet rich in fat, 14 as well as supported the validity of studying the effects of green tea extract in diet-induced obese zebrash. 11,14 Hence, in the present study, the chemical compositions of MLGT were investigated for the rst time and its health benet, the hypolipidemic bioactivity, was evaluated by using the diet-induced obese zebrash.…”

Section: Introductionmentioning

confidence: 93%

“…Zebrash were placed in the 6-well cell culture plates at a density of 15 zebrash in 4 mL of solution per group for treatment. 16,17 Finally, all zebrash were xed with 4% paraformaldehyde at 4 C overnight and dehydrated by 60% isopropanol for 30 min aer three washes of 1Â phosphatebuffered saline (PBS). In order to compare lipid levels in different experimental groups, ORO staining was conducted.…”

Section: Hypolipidemic Activity Of Mlgt In a Zebrash Modelmentioning

confidence: 99%

Comprehensive evaluation of the composition of Mingshan Laochuancha green tea and demonstration of hypolipidemic activity in a zebrafish obesity model

Xiao

Gao

2019

RSC Adv.

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“…Some important advantages have been associated with the zebrafish in terms of their use in research, such as the small size, easy maintenance, low cost of breeding, and high reproductive rate. In addition, its genome has already been sequenced, and it presents important homology with mammals, besides the morphological and molecular basis of tissues and organs similar to humans . So, zebrafish is emerging as a predictive vertebrate animal model for in vivo assessment of drug efficacy, toxicity, and safety .…”

Section: Introductionmentioning

confidence: 99%

“…In addition, its genome has already been sequenced, and it presents important homology with mammals, besides the morphological and molecular basis of tissues and organs similar to humans . So, zebrafish is emerging as a predictive vertebrate animal model for in vivo assessment of drug efficacy, toxicity, and safety . Besides, some studies have evaluated the ability of zebrafish larvae at different stages of ripening to generate xenobiotic metabolites .…”

Section: Introductionmentioning

confidence: 99%

Is zebrafish (Danio rerio) a tool for human‐like metabolism study?

Anselmo

Sardela

Matias

et al. 2017

Drug Testing and Analysis

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One of the greatest challenges in anti-doping science is the large number of substances available and the difficulty in finding the best analytical targets to detect their misuse. Therefore, metabolism studies involving prohibited substances are fundamental. However, metabolism studies in humans could face an important ethical bottleneck, especially for non-approved substances. An emerging model for metabolism assessment is the zebrafish, due to its genetic similarities with humans. In the present study, the ability of adult zebrafish to produce metabolites of sibutramine and stanozolol, substances with a well-known metabolism that are widely used as doping agents in sports, was evaluated. They represent 2 of the most abused classes of doping agents, namely, stimulants and anabolic steroids. These are classes that have been receiving attention because of the upsurge of synthetic analogues, for which the side effects in humans have not been assessed. The samples collected from the zebrafish tank water were hydrolysed, extracted by solid-phase extraction, and analysed by liquid chromatography with high resolution mass spectrometry (LC-HRMS). Adult zebrafish could produce several sibutramine and stanozolol metabolites, including demethylated, hydroxylated, dehydroxylated, and reduced derivatives, all of which have already been detected in human urine. This study demonstrates that adult zebrafish can absorb, oxidise, and excrete several metabolites in a manner similar to humans. Therefore, adult zebrafish seem to be a very promising tool to study human-like metabolism when aiming to find analytical targets for doping control.

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Rapid analysis of hypolipidemic drugs in a live zebrafish assay

Cited by 47 publications

References 29 publications

A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs

A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs

Comprehensive evaluation of the composition of Mingshan Laochuancha green tea and demonstration of hypolipidemic activity in a zebrafish obesity model

Is zebrafish (Danio rerio) a tool for human‐like metabolism study?

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