Rapid Analysis of Oligonucleotides on a Planar Microfluidic Chip

Evstrapov, A. A.; Bulyanitsa, A. L.; Курочкин, В. Е.; Petryakov, A. O.; Rudnitskaya, G. E.; Sal'nikova, T. A.; Alekseev, Ya. I.

doi:10.1023/b:janc.0000030870.01412.71

Cited by 6 publications

(3 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are several commercial products available for DNA and RNA separations by Caliper, Agilent, Shimadzu, and Hitachi. Several review articles have been published on applications of microchips for DNA analysis [2,[67][68][69][70][71].…”

Section: Analysis Of Dnamentioning

confidence: 99%

Applications of capillary electrophoresis on microchip

Dolnı́k¹,

Liu

2005

J of Separation Science

107

View full text Add to dashboard Cite

CE on microchip is an emerging separation technique that has attracted wide attention and gained considerable popularity. Because of miniaturization of the separation format, CE on chip typically offers shorter analysis time and lower reagent consumption with potential development of portable analytical instrumentation. This review with 143 references is focused on proteins and peptides analysis, DNA separation including fragment sizing, genotyping, mutation detection and sequencing, and also the analysis of low-molecular-weight compounds, namely explosive residues and warfare agents, pharmaceuticals and drugs of abuse, and various small molecules in body fluids.

show abstract

Section: Analysis Of Dnamentioning

confidence: 99%

Applications of capillary electrophoresis on microchip

Dolnı́k¹,

Liu

2005

J of Separation Science

107

View full text Add to dashboard Cite

show abstract

“…This has lead to the production of a few commercial instruments using glass microchips instead of capillaries or slab gels, which have been discussed elsewhere in detail [3]. Table 1 provides a basic operational and performance comparison between three electrophoretic platforms typically used in DNA analysis namely, slab gel, CAE, and microchip electrophoresis (microchip CE).…”

Section: Introductionmentioning

confidence: 99%

High resolution DNA separations using microchip electrophoresis

Sinville

Soper

2007

J of Separation Science

View full text Add to dashboard Cite

Planar microfluidic devices have emerged as effective tools for the electrophoretic separation of a variety of different DNA inputs. The advancement of this miniaturized platform was inspired initially by demands placed on electrophoretic performance metrics by the human genome project and has provided a viable alternative to slab gel and even capillary formats due to its ability to offer high resolution separations of nucleic acid materials in a fraction of the time associated with its predecessors, consumption of substantially less sample and reagents while maintaining the ability to perform many separations in parallel for realizing ultra-high throughputs. Another compelling advantage of this separation platform is that it offers the potential for integrating front-end sample preprocessing steps onto the separation device eliminating the need for manual sample handling. This review aims to compile a recent survey of various electrophoretic separations using either glass or polymer-based microchips in the areas of genotyping and DNA sequencing as well as those involving the growing field of DNA-based forensics.

show abstract

“…Rapid development of microfluidic chip technology has allowed many chemical and biomedical analyses to be performed on a palm-size plastic or glass chip [1][2][3][4][5] with significantly improved resolution and efficiency [6,7]. However, to date, with a few notable exceptions, most research in microfluidic chips has focused on the analysis of simple and pre-purified biological samples, such as oligonucleotides [8], DNA [9,10], RNA [11,12] and single cell analysis [13,14].…”

Section: Introductionmentioning

confidence: 99%

A 3D electrokinetic flow structure of solution displacement in microchannels for on-chip sample preparation applications

Shao¹,

Ren

Schneider³

2006

J. Micromech. Microeng.

View full text Add to dashboard Cite

Electroosmotic flow with solution displacement in microchannels is often encountered in many lab-on-a-chip devices where washing procedures are designed using one solution to displace another different solution. In order to investigate the detailed flow structures for a displacement process between two different electrolyte solutions, a three-dimensional numerical model is developed in this paper. KCl solution and LaCl3 solution are used as sample solutions. A 2 mm long straight microchannel with a rectangular cross-sectional area (height 100 µm and width 200 µm) was employed in this study. The governing equations of the applied electrical field, flow field and concentration field are numerically solved based on a finite-control-volume scheme. The fluid flow coupled equations are solved using the semi-implicit method for pressure-linked equation (SIMPLE) algorithm. The observed electrokinetic flow structures include back flow in the center of the channel and distortion of the plug-like electroosmotic velocity profile, which are investigated in detail. It is found that distortion in the flow field is due to the induced pressure gradient, which results from the nonuniformity of electroosmotic mobilities and electrical conductivities of the two solutions. Finally, the displacement between a pair of different solutions, distilled ultra-filtered (DIUF) water and LaCl3 solution, is briefly studied.

show abstract

Rapid Analysis of Oligonucleotides on a Planar Microfluidic Chip

Cited by 6 publications

References 38 publications

Applications of capillary electrophoresis on microchip

Applications of capillary electrophoresis on microchip

High resolution DNA separations using microchip electrophoresis

A 3D electrokinetic flow structure of solution displacement in microchannels for on-chip sample preparation applications

Contact Info

Product

Resources

About