Rapid and ecofriendly UPLC quantification of Remdesivir, Favipiravir and Dexamethasone for accurate therapeutic drug monitoring in Covid-19 Patient’s plasma

Recent analytical methodologies for the determination of anti-covid-19 drug therapies in various matrices: a critical review

“…According to the FDA guidelines, the method proved be accurate, precise, selective, sensitive, and green (Eco-Scale score = 83). 59…”

“…According to the FDA guidelines, the method proved be accurate, precise, selective, sensitive, and green (Eco-Scale score = 83). 59 Molnupiravir, favipiravir, and ritonavir in pure form and in pharmaceutical dosage forms: Ibrahim et al have developed a simple HPTLC method for the quantication of the orally active antivirals MLP, RTN, and FVP which were used in the treatment regimens of Covid-19. Samples were spotted on Silica gel 60F254 thin layer chromatography plates and then developed using methylene chloride : ethyl acetate : methanol : 25% ammonia (6 : 3 : 4 : 1,v/v/v/v) as a mobile phase and the chromatogram was visualized at 289 nm.…”

Analytical Performance and Greenness Evaluation of Five Multi-Level Design Models Utilized for Impurity Profiling of Favipiravir, a Promising COVID-19 Antiviral Drug

“…From this perspective, there is a persistent need for the simultaneous development of sensitive analytical methods for FVR determination with its process-related impurities. A review of the literature revealed few analytical methodologies that were reported for FVR determination using chromatographic techniques [13][14][15][16][17][18][19][20][21][22][23][24][25]. Three spectrofluorimetric methods [19,26,27] and two electrochemical methods [28,29] were reported for the determination of FVR.…”

A validated eco-friendly HPLC-FLD for analysis of the first approved antiviral remdesivir with other potential add-on therapies for COVID-19 in human plasma and pharmaceuticals

“…The method's linearity was evaluated using seven different concentrations spanning the ranges of 20-4000 ng mL −1 for both RDV and LVX, and 15-2000 ng mL −1 for HCQ in the pure form and 40-4000, 20-1500, and 100-4000 ng mL −1 for RDV, HCQ, and LVX, correspondingly, in plasma. As the proposed method's linearity ranges in plasma covered the C max for all the cited drugs, it could be extended to application in real patients' plasma, as the C max was 0.057-4.420 mg mL −1 , 50.3 ng mL −1 and 2.8-5.2 mg mL −1 for RDV, HCQ, and LVX; 37,41,42 respectively. Regression equation parameters are presented in Table 4.…”