Rapid and High‐Throughput Forensic Short Tandem Repeat Typing Using a 96‐Lane Microfabricated Capillary Array Electrophoresis Microdevice^*

Abstract

A 96-channel microfabricated capillary array electrophoresis (muCAE) device was evaluated for forensic short tandem repeat (STR) typing using PowerPlex 16 and AmpFlSTR Profiler Plus multiplex PCR systems. The high-throughput muCAE system produced high-speed <30-min parallel sample separations with single-base resolution. Forty-eight previously analyzed single-source samples were accurately typed, as confirmed on an ABI Prism 310 and/or the Hitachi FMBIO II. Minor alleles in 3:1 mixture samples containing female and male DNA were reliably typed as well. The instrument produced full profiles from sample DNA down to 0.17 ng, a threshold similar to that found for the ABI 310. Seventeen nonprobative samples from various evidentiary biological stains were also correctly typed. The successful application of the muCAE device to actual forensic STR typing samples is a significant step toward the development of a completely integrated STR analysis microdevice.

Recent advances in the applications of CE to forensic sciences (2005–2007)

“…In addition, Yeung et al [56] reported on high-throughput DNA analysis presenting a 96-channel microfabricated capillary array system, which was evaluated for STR analysis. The system produced ,30 min parallel separations with single-base resolution and high accuracy, as proved by parallel analysis with validated systems.…”

The development of mini pentameric STR loci for rapid analysis of forensic DNA samples on a microfluidic system

“…Thus, these issues with short separation channels could be alleviated through the use of a smaller set of short amplicons that do not require such long separation channels. Such a sample set would be ideal for application with microfluidic devices and would fit the need for a rapid onsite screening tool [25].…”

Fluorescence energy transfer‐labeled primers for high‐performance forensic DNA profiling

“…A high-throughput microfabricated capillary array electrophoresis (mCAE) STR analysis system [9] is used for evaluating these reagents. The spectroscopic properties and performance of each ET-labeled primer and ETlabeled single-locus PCR product(s) were compared to those labeled with their corresponding single fluorescent dyes.…”