Rapid and Label-Free Electrochemical Detection of Fumonisin-B1 Using Microfluidic Biosensing Platform Based on Ag-CeO₂ Nanocomposite

Abstract

Food contamination by mycotoxins is a major problem. Fumonisin-B1 (FU-B1) is one of the most abundantly found mycotoxins in products such as corn, rice, maize, and wheat, causing some of the most contagious diseases, such as pulmonary edema, hydrothorax, and immunosuppression, and is possibly carcinogenic. Its rapid and early detection in food products is therefore vital. We have fabricated an electrochemical microfluidic biosensing platform to detect FU-B1 with silver-ceria nanocomposite using maskless lithography. Structural characterizations confirmed nanocubes formation (CeO 2 ) and spherical structures (Ag) with no impurities. The resulting nanobiochip showed an excellent linear response to antigen-FU-B1. linear detection was obtained from 10 pg ml −1 −100 ng ml −1 with high sensitivity as 7.33 μA (log (ng. ml −1 )) −1 cm −2 . Limit of detection and limit of quantification were obtained as 1.5 pg. ml −1 , and 3.9 pg. ml −1 , respectively. Reynold's number was found to be 0.79, showing the fluid's laminar flow inside the channel. Interference studies showed specificity toward FU-B1 detection. Applicability in real food samples was assessed using spiked corn samples and showed recovery of up to 85% with excellent linear response. These results indicate that an electrochemical microfluidic nanobiochip can be used for rapid, label-free, and highly sensitive detection of antigen FU-B1.

Co-Application of Silver Nanoparticles and Symbiotic Fungus Piriformospora indica Improves Secondary Metabolite Production in Black Rice

“…The obtained XRD spectra showed the XRD peaks 2θ = 38°, 44.14°, 64.24°, 77.24°, 81.36°, 97.66°, respectively, with an intense peak at 2θ = 38°. Figure 3 a shows the face-centered cubic (FCC) crystal reflection planes of the four faces of crystalline AgNPs [ 1 , 2 , 16 ]. XRD peaks confirmed the formation of FCC crystallographic planes which aligned with the standard silver peaks ( Figure 3 a).…”

“…UV-Vis absorption spectrophotometry showed a peak around the 400 nm wavelength, characteristic of well-dispersed AgNPs ( Figure 3 b). The SPR peak of chemically synthesized AgNPs has been obtained at the 400 nm wavelength [ 1 ]. Figure 3 c shows the Zeta potential of the silver nanoparticles, which was around −30 Mv.…”

“…The evolution of nanotechnology has strengthened the effective use of nanoparticles and has emerged as a radical tool for enhancing agricultural practices and moving to a more sustainable and resilient farming sector [ 1 , 2 , 3 ]. Nanoscale Particles (NSPs) are atomic or molecular-sized particles of dimension between 1 nm and 100 nm that can modify their physiochemical properties compared to bulk material [ 4 , 5 ].…”

MIP-Based Sensor for Detection of Gut Microbiota Derived Trimethylamine

“…Synthesis of CeO 2 nps.-CeO 2 nps are synthesized using sodium hydroxide and cerous nitrate hexahydrate using the co-precipitation method. 40,41 0.3 M NaOH and 0.1 M cerous nitrate hexahydrate solutions were prepared using distilled water. NaOH solution was added dropwise in the solution of cerous hexahydrate under constant stirring until basic pH was obtained (pH [12][13][14].…”

Rapid and label-free detection of Aflatoxin-B1 via microfluidic electrochemical biosensor based on manganese (III) oxide (Mn3O4) synthesized by co-precipitation route at room temperature

“…Fabrication of microfluidic channel. The microfluidic channel was created by mask-less lithography as explained in our previously published article [46]. Three inches of the silicon wafer was used as a substrate to fabricate a microfluidic channel, which was further coated with negative photoresist SU8 using a spin coater at high RPM for 5 min.…”