Rapid and low-cost diagnosis of Japanese yam mosaic virus infection in Chinese yam (Dioscorea polystachya) leaves by a print-capture RT-PCR

Mochizuki, Tomofumi; Iwamoto, Itsuki; Atarashi, Aya; Ohki, Satoshi T.; Kikukawa, Hiroyuki

doi:10.1007/s10327-017-0719-6

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…Because the tubers of Chinese yam are delicate and become deformed when they encounter obstacles in the soil, it is important to identify candidate sites with optimal growth conditions. The Chinese yam is susceptible to various viruses including the Japanese yam mosaic virus (Fuji and Nakamae 1999;Mochizuki et al 2017), Yam mild mosaic virus (Fuji et al 2001), Chinese yam necrotic mosaic virus (Fukumoto and Tochihara 1978) and Broad bean wilt virus 2 (Kondo et al 2005). Leaf miners (Acrolepiopsis spp.)…”

Section: Opportunities For the Global Cultivation Of Chinese Yammentioning

confidence: 99%

An underutilized orphan tuber crop—Chinese yam : a review

Epping¹,

Laibach

2020

Planta

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Main conclusion The diversification of food crops can improve our diets and address the effects of climate change, and in this context the orphan crop Chinese yam shows significant potential as a functional food. Abstract As the effects of climate change become increasingly visible even in temperate regions, there is an urgent need to diversify our crops in order to address hunger and malnutrition. This has led to the re-evaluation of neglected species such as Chinese yam (Dioscorea polystachya Turcz.), which has been cultivated for centuries in East Asia as a food crop and as a widely-used ingredient in traditional Chinese medicine. The tubers are rich in nutrients, but also contain bioactive metabolites such as resistant starches, steroidal sapogenins (like diosgenin), the storage protein dioscorin, and mucilage polysaccharides. These health-promoting products can help to prevent cardiovascular disease, diabetes, and disorders of the gut microbiome. Whereas most edible yams are tropical species, Chinese yam could be cultivated widely in Europe and other temperate regions to take advantage of its nutritional and bioactive properties. However, this is a laborious process and agronomic knowledge is fragmented. The underground tubers contain most of the starch, but are vulnerable to breaking and thus difficult to harvest. Breeding to improve tuber shape is complex given the dioecious nature of the species, the mostly vegetative reproduction via bulbils, and the presence of more than 100 chromosomes. Protocols have yet to be established for in vitro cultivation and genetic transformation, which limits the scope of research. This article summarizes the sparse research landscape and evaluates the nutritional and medical applications of Chinese yam. By highlighting the potential of Chinese yam tubers, we aim to encourage the adoption of this orphan crop as a novel functional food.

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Section: Opportunities For the Global Cultivation Of Chinese Yammentioning

confidence: 99%

An underutilized orphan tuber crop—Chinese yam : a review

Epping¹,

Laibach

2020

Planta

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“…The virus is typically transmitted through vegetative propagation of seed tubers but may be transmitted by aphid vectors, A. gossypii, and Myzus persicae, in a non-persistent manner [31,66]. Symptoms of JYMV infection are similar to YMV, such as mosaic, greenbanding of leaves, yellow stripes, and chlorosis, that can cause significant yield loss [64,67] Fuji and Nakamae [31] reported the use of two methods, RT-PCR and double-antibody sandwich ELISA (DAS-ELISA) for the detection of JYMV.…”

Section: Genus Potyvirusmentioning

confidence: 99%

“…A rapid and low-cost detection method for JYMV based on print-capture RT-PCR was reported by Mochizuki et al [66]. Nucleic acid is captured onto a nitrocellulose membrane from leaf sap and recovered through an elution solution.…”

Section: Genus Potyvirusmentioning

confidence: 99%

A Review of Viruses Infecting Yam (Dioscorea spp.)

Luo

Podolyan

Kidanemariam

et al. 2022

Viruses

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Yam is an important food staple for millions of people globally, particularly those in the developing countries of West Africa and the Pacific Islands. To sustain the growing population, yam production must be increased amidst the many biotic and abiotic stresses. Plant viruses are among the most detrimental of plant pathogens and have caused great losses of crop yield and quality, including those of yam. Knowledge and understanding of virus biology and ecology are important for the development of diagnostic tools and disease management strategies to combat the spread of yam-infecting viruses. This review aims to highlight current knowledge on key yam-infecting viruses by examining their characteristics, genetic diversity, disease symptoms, diagnostics, and elimination to provide a synopsis for consideration in developing diagnostic strategy and disease management for yam.

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