Rapid and noninvasive diagnosis of the presence and severity of coronary heart disease using 1H-NMR-based metabonomics

Abstract

Although a wide range of risk factors for coronary heart disease have been identified from population studies, these measures, singly or in combination, are insufficiently powerful to provide a reliable, noninvasive diagnosis of the presence of coronary heart disease. Here we show that pattern-recognition techniques applied to proton nuclear magnetic resonance (1H-NMR) spectra of human serum can correctly diagnose not only the presence, but also the severity, of coronary heart disease. Application of supervised partial least squares-discriminant analysis to orthogonal signal-corrected data sets allows >90% of subjects with stenosis of all three major coronary vessels to be distinguished from subjects with angiographically normal coronary arteries, with a specificity of >90%. Our studies show for the first time a technique capable of providing an accurate, noninvasive and rapid diagnosis of coronary heart disease that can be used clinically, either in population screening or to allow effective targeting of treatments such as statins.

The metabolomic profile during isoflurane anesthesia differs from propofol anesthesia in the live rodent brain

“…An alternative method for characterizing the metabolic state of the brain or biofluids is by way of 'metabolomics' (Brindle et al, 2002;Coen et al, 2008). The field of metabolomics is defined as the study of small-molecular-weight molecules involved in metabolism, with a focus on how their levels change and/or which metabolites are more involved in response to different drugs or pathologies (Ebbels and Cavill, 2009).…”

Benzo(a)pyrene-induced metabolic responses in Manila clam Ruditapes philippinarum by proton nuclear magnetic resonance (1H NMR) based metabolomics

“…Among these post-genomic techniques, proton nuclear magnetic resonance ( 1 H NMR) spectroscopy-based metabolomics can perform a systematic study on the global metabolic profile, especially the low molecular-weight metabolites (<1000 Da) to characterize the impact on specific cellular processes (Daviss, 2005). Such study of metabolic profiling has been widely used in multiple research fields including drug toxicity, inborn disease diagnosis, functional genomics, and environmental toxicology (Brindle et al, 2002;Bundy et al, 2004;Wu et al, 2005;Viant et al, 2006a,b;Liu et al, 2011). Moreover, NMR spectroscopy is rapid and rich in structural and quantitative information and allows the metabolites to be analyzed simultaneously (Lindon et al, 2000;Wu et al, 2005).…”

Metabolomic mapping of atypical antipsychotic effects in schizophrenia

“…[13][14][15][16][17][18][19][20] Lipidomics is a branch of metabolomics where focus is specifically targeted on quantifying a wide range of polar and nonpolar lipid metabolites enabling a more comprehensive assessment of human lipid biochemical pathways than was previously practical. [21][22][23] We used this platform to map and compare global lipid changes in schizophrenia and upon treatment with three commonly used atypical antipsychotic drugs.…”