Rapid and robust MALDI-TOF MS techniques for microbial identification: a brief overview of their diverse applications

Jang, Kyoung‐Soon; Kim, Young Hwan

doi:10.1007/s12275-018-7457-0

Cited by 131 publications

(77 citation statements)

References 85 publications

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“…Today, MS-based analysers are in routine use, such as the Bruker BioTyper (Bruker Daltonics) and VITEK MS Plus (bioMérieux), both detecting MS1 spectra fingerprint of most abundant proteins present in a wide array of microorganisms [24,25,26]. The US Food and Drug Administration (FDA) has issued regulatory approval for using mass spectrometry-based platform MALDI-TOF MS for routine identification of pathogenic microbes from human specimens in clinical microbiology laboratories [27,23].…”

Section: Field Of Proteomics Also Offers Methods For Microbial Identimentioning

confidence: 99%

Identification of pathogens from native urine samples by MALDI-TOF/TOF tandem mass spectrometry

Starčević

Oros

Ceprnja

et al. 2020

Preprint

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Background: Reliable high-throughput microbial pathogen identification in human urine samples is crucial for patients with cystitis symptoms. Currently employed methods are time-consuming and could lead to unnecessary or inadequate antibiotic treatment. Purpose of this study was to assess the potential of mass spectrometry for uropathogen identification from a native urine sample. Methods: In total, 16 urine samples having more than 10 5 CFU/mL were collected from clinical outpatients. These samples were analysed using standard urine culture methods, followed by 16S rRNA gene sequencing serving as control and here described culture-independent MALDI-TOF/TOF MS method being tested. Results: Here we present advantages and disadvantages of bottom-up proteomics, using MALDI-TOF/TOF tandem mass spectrometry, for culture-independent identification of uropathogens (e.g. directly from urine samples). The direct approach provided reliable identification of bacteria at the genus level in monobacterial samples. Taxonomic identifications obtained by proteomics were compared both to standard urine culture test used in clinics and genomic test based on 16S rRNA sequencing. Conclusions: Our findings indicate that mass spectrometry has great potential as a reliable high-throughput tool for microbial pathogen identification in human urine samples. In this case, the MALDI-TOF/TOF, was used as an analytical tool for the determination of bacteria in urine samples, and the results obtained emphasize high importance of storage conditions and sample preparation method impacting reliability of MS2 data analysis. The proposed method is simple enough to be utilized in existing clinical settings and is highly suitable for suspected single organism infectious etiologies. Further research is required in order to identify pathogens in polymicrobial urine samples. Keywords: urine, sample preparation, pathogen identification, proteomics, MALDI-TOF/TOF, 16S rRNA sequencing

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Section: Field Of Proteomics Also Offers Methods For Microbial Identimentioning

confidence: 99%

Identification of pathogens from native urine samples by MALDI-TOF/TOF tandem mass spectrometry

Starčević

Oros

Ceprnja

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a powerful diagnostic tool (Singhal et al, 2015;Sanguinetti & Posteraro, 2017;Jang & Kim, 2018) that can be applied for accurate identification of clinically-important microorganisms at low cost and short turnaround time. Unlike serological and molecular assays, MALDI-TOF MS does not require pathogen-specific reagents, such as primer, antigen, and antibody.…”

Section: Proteomic Analysismentioning

confidence: 99%

Recent update in diagnosis and treatment of human pythiosis

Chitasombat

Jongkhajornpong

Lekhanont

et al. 2020

PeerJ

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Human pythiosis is an infectious condition with high morbidity and mortality. The causative agent is the oomycete microorganism Pythium insidiosum. The pathogen inhabits ubiquitously in a wet environment, and direct exposure to the pathogen initiates the infection. Most patients with pythiosis require surgical removal of the affected organ, and many patients die from the disease. Awareness of pythiosis among healthcare personnel is increasing. In this review, we summarized and updated information on the diagnosis and treatment of human pythiosis. Vascular and ocular pythiosis are common clinical manifestations. Recognition of the typical clinical features of pythiosis is essential for early diagnosis. The definitive diagnosis of the disease requires laboratory testing, such as microbiological, serological, molecular, and proteomic assays. In vascular pythiosis, surgical intervention to achieve the organism-free margin of the affected tissue, in combination with the use of antifungal drugs and P. insidiosum immunotherapy, remains the recommended treatment. Ocular pythiosis is a serious condition and earliest therapeutic penetrating keratoplasty with wide surgical margin is the mainstay treatment. Thorough clinical assessment is essential in all patients to evaluate the treatment response and detect an early sign of the disease recurrence. In conclusion, early diagnosis and proper management are the keys to an optimal outcome of the patients with pythiosis.

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“…Matrix-assisted laser desorption ionisation-time of ight mass spectrometry (MALDI-TOF MS) is an emerging high-throughput technology with broad potential in clinical microbial identi cation because of its high resolution, speed, sensitivity, and accuracy [1][2][3]. Microorganism detection is based on databases of known bacteria.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Autof MS1000 mass spectrometer in the identification of clinical isolates

Zhang

Yuan

et al. 2020

Preprint

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Background: To evaluate the accuracy and performance of the Autof MS1000 mass spectrometer in bacteria and yeast identification, 2,342 isolates were obtained from microbial cultures of clinical specimens (e.g. blood, cerebrospinal fluid, respiratory tract samples, lumbar puncture fluid, wound samples, stool, and urine) collected in 2019 in Henan Provincial People’s Hospital. Repetitive strains from the same patient were excluded. We tested the Autof MS1000 and Bruker Biotyper mass spectrometry systems and the classical biochemical identification system VITEK 2/API 20C AUX. Inconsistencies in strain identification among the three systems were identified by 16S rDNA and gene sequencing.Results: At the species level, the Autof MS1000 and Bruker Biotyper systems had isolate identification accuracies of 98.9% and 98.5%, respectively. At the genus level, the Autof MS1000 and Bruker Biotyper systems were 99.7% and 99.4% accurate, respectively. The instruments did not significantly differ in identification accuracy at either taxonomic level. The frequencies of unreliable identification were 1.1% (26/2,342) for the Autof MS1000 and 1.5% (34/2,342) for the Bruker Biotyper. In vitro experiments demonstrated that the coincidence rate of the Autof MS1000 mass spectrometer in the identification of five types of bacteria was >93%, the identification error rate was <3%, and the no identification rate was 0. This indicates that the Autof MS1000 system is acceptable for identification.Conclusions: The Autof MS1000 mass spectrometer can be utilised to identify clinical isolates. However, an upgradation of the database is recommended to correctly identify rare strains.

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Rapid and robust MALDI-TOF MS techniques for microbial identification: a brief overview of their diverse applications

Cited by 131 publications

References 85 publications

Identification of pathogens from native urine samples by MALDI-TOF/TOF tandem mass spectrometry

Identification of pathogens from native urine samples by MALDI-TOF/TOF tandem mass spectrometry

Recent update in diagnosis and treatment of human pythiosis

Evaluation of the Autof MS1000 mass spectrometer in the identification of clinical isolates

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