Rapid and selective electrochemical sensing of bacterial pneumonia in human sputum based on conductive polymer dot electrodes

Jo, Hyeong Jun; Ryu, Jea Sung; Robby, Akhmad Irhas; Kim, Yang Soo; Chung, Hyun Jung; In, Insik

doi:10.1016/j.snb.2022.132084

Cited by 15 publications

(13 citation statements)

References 34 publications

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“…), in the design of various electrochemical biosensors. [ 20 , 21 , 22 ]. In this regard, we designed a biocompatible nanocomposite from sulfonated starch grafted to polyaniline and graphene for the immobilization of tyrosinase.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, PANI displays two redox couples which ease the charge transfer between an enzyme and a polymer. Due to the great electrochemical properties along with in vivo biocompatibility, PANI-based nanocomposites can be used to detect a negligible amount of biomolecules with high sensitivities and fast responses [ 18 , 19 , 20 , 21 , 22 ]. To enhance the good immobilization of enzymes on the PANI, the copolymerization method with functionalized monomers and/or modified natural polymers can be used.…”

Section: Introductionmentioning

confidence: 99%

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Sulfonated Starch-Graft-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization

et al. 2022

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The interaction of tyrosinase with sulfonated starch-graft-polyaniline@graphene (SSt-g-PANI@G) nanocomposite was investigated by electrochemical methods. The activity of the immobilized tyrosinase (Tyase) was proved by the electrochemical detection of three substrates (L-dopa, caffeic acid, and catechol). The SSt-g-PANI@G nanocomposite was characterized by Fourier-transform infrared spectra (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy-dispersive X-ray analysis (EDX), and thermogravimetric analysis (TGA). To immobilize tyrosinase on the surface of the nanocomposite, a simple drop-casting technique was used. The presence of sulfuric acid and hydroxyl groups in SSt, amine groups in PANI, and high surface-to-volume ratio and electrical conductivity of graphene in the prepared nanocomposite led to good enzyme immobilization on the electrode surface. The modified electrode showed a suitable catalytic effect on the electrochemical redox agent, compared with the bare electrode. The peak current responses for three substrates were studied with a calibration curve derived using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). In addition, the fabricated SSt-g-PANI@G/Tyase/GCE showed a more suitable response to catechol, L-dopa, and caffeic acid substrates, respectively.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sulfonated Starch-Graft-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization

et al. 2022

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“…Although a couple of pathogenic ARB biosensors have been developed to help medical personnel early identify infection risks and disease progress, their detection process is not simple enough. Most of them employ electrochemical sensing strategies because bacteria themselves with highly negative charges are good conductors, and thereby the solution conductivity near the sensing interface easily changes when samples are in the presence of ARB [18]. Moreover, electron respiratory chains in bacteria could be exploited, when they interact with appropriate substrates dynamic electrical signals will appear [19].…”

Section: Lack Of On-site Reporting Abilitymentioning

confidence: 99%

Challenges and strategies in developing biosensors for immediate and specific detection of antibiotic-resistant bacteria

Hao

2024

Third International Conference on Biological Engineering and Medical Science (ICBioMed2023)

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The increase of antibiotic-resistant bacteria is likely to exceed people's common awareness, and it poses a huge confusion for clinical medication timing. Accurate identification and timely reporting of antibiotic-resistant bacteria will reduce infections and deaths. While the developed identification methods of antibiotic-resistant bacteria are helpful to decrease possible danger, there is a dearth of antibiotic-resistant bacteria biosensors capable of dealing with public health emergencies and precisely distinguishing particular antibiotic-resistant bacteria in the field. This paper analyzes the challenges of building specific and immediate biosensors for antibiotic-resistant bacteria from both the recognition elements and sensing methods, and accordingly proposes the most promising solutions to these challenges. It has been found that the specificity and availability of targeting molecules and targets for antibiotic-resistant bacteria are major limits to develop antibiotic-resistant bacteria biosensors. The lack of on-site reporting capabilities further hinders the popularization of existing antibiotic-resistant bacteria biosensors. Through comparison among different recognition elements, aptamer may be the most promising targeting molecules for future antibiotic-resistant bacteria biosensors design. In addition, optically paper-based or membrane-based biosensors are recommended because of their simplicity and potential of rapid translation into field applications.

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“…[ 98 ] Moreover, selective detection of bacteria from different species can be achieved through sensing nucleic acids. For instance, carbapenem‐resistant K. pneumonia and carbapenem‐sensitive K. pneumonia can be discriminated by detecting the probe‐16S rRNA gene complex after incubation with carbapenem for only 4 h. [ 99 ] Real‐time sequencing has also been realized to a certain extent, with MinION providing comprehensive identification of species in mixed microbial communities through sequencing the complete 16S rRNA gene. When combined with PCR amplification of the 16S rRNA gene, Staphylococcus aureus , Escherichia coli , Enterobacter faecalis , Pseudomonas aeruginosa , and bacterial pathogens have been successfully identified in positive blood culture bottles.…”

Section: Detection Of Pathogens and Microorganisms With Nanopore Sequ...mentioning

confidence: 99%

Application of Biological Nanopore Sequencing Technology in the Detection of Microorganisms^†

Zhang

Huang

2023

Chin. J. Chem.

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Comprehensive SummaryEnvironmental pollution and the spread of pathogenic microorganisms pose a significant threat to the health of humans and the planet. Thus, understanding and detecting microorganisms is crucial for maintaining a healthy living environment. Nanopore sequencing is a single‐molecule detection method developed in the 1990s that has revolutionized various research fields. It offers several advantages over traditional sequencing methods, including low cost, label‐free, time‐saving detection speed, long sequencing reading, real‐time monitoring, convenient carrying, and other significant advantages. In this review, we summarize the technical principles and characteristics of nanopore sequencing and discuss its applications in amplicon sequencing, metagenome sequencing, and whole‐genome sequencing of environmental microorganisms, as well as its in situ application under some special circumstances. We also analyze the advantages and challenges of nanopore sequencing in microbiology research. Overall, nanopore sequencing has the potential to greatly enhance the detection and understanding of microorganisms in environmental research, but further developments are needed to overcome the current challenges.This article is protected by copyright. All rights reserved.

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Rapid and selective electrochemical sensing of bacterial pneumonia in human sputum based on conductive polymer dot electrodes

Cited by 15 publications

References 34 publications

Sulfonated Starch-Graft-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization

Sulfonated Starch-Graft-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization

Challenges and strategies in developing biosensors for immediate and specific detection of antibiotic-resistant bacteria

Application of Biological Nanopore Sequencing Technology in the Detection of Microorganisms^†

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Rapid and selective electrochemical sensing of bacterial pneumonia in human sputum based on conductive polymer dot electrodes

Cited by 15 publications

References 34 publications

Sulfonated Starch-Graft-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization

Sulfonated Starch-Graft-Polyaniline@Graphene Electrically Conductive Nanocomposite: Application for Tyrosinase Immobilization

Challenges and strategies in developing biosensors for immediate and specific detection of antibiotic-resistant bacteria

Application of Biological Nanopore Sequencing Technology in the Detection of Microorganisms†

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Product

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About

Application of Biological Nanopore Sequencing Technology in the Detection of Microorganisms^†