Rapid and selective theophylline serum and saliva assay by means of high pressure liquid chromatography

Abstract

A rapid, selective and sensitive high pressure liquid chromatographic (HPLC) method for tile determination of theophylline in human serum (or plasma) and saliva was developed.When t, sing 0.5 ml of serum, concentrations down to o.4 mg.I -~ could be accurately measured. Each sample requires only about I5 minutes for the completion of the assay, including sample preparation. The actual chromatography time is about 8 minutes. The theophylline metabolites and other xanthines, as theobromine, caffeine (and its metabolite paraxanthine) are well separated. The sensitivity, precision and accuracy are sufficient for routine monitoring of therapeutic theophylline serum levels in patients (approximately lo to 20 rag.l-t). Rehability of the method was demonstrated during analysis of about 3000 samples on the same column. (Pharm. Weekblad Sci. Ed. 2,(49)(50)(51)(52)(53)

Pharmacokinetics of sustained release theophylline in low and high multidose regimens.

“…The theophylline concentrations in serum were determined by using a HPLC method (Jonkman, Schoenmaker et al, 1980). The area under the serum theophylline concentration time curve was calculated from day 6 at 8.00 h to day 7 at 16.00 h. The serum clearance was calculated by dividing the dose per time interval by the steady state serum concentrations, and\ was expressed per kg body weight.…”

Respiratory stimulant effects of adenosine in man after caffeine and enprofylline.

“…Plasma caffeine and enprofylline concentrations were determined by a h.p.l.c.-method. (Jonkman et al, 1980;Edholm & Heintz, 1982). The data were analysed by paired Student's t-test (twotailed), and all results are presented as mean values ± s.e.mean.…”

A simple, fast and reliable liquid chromatographic method for the determination of theophylline in urine, saliva and plasma samples

“…Although in recent years, theophylline has been determined using a number of techniques including high-performance liquid chromatography (HPLC) (2-10), spectrophotometry (11-13), thin layer chromatography (TLC) (14), high-performance thin layer chromatography (HPTLC) (15), gas-liquid chromatography (GLC) (16), but some of them suffer from poor resolution, long chromatographic run time and tedious pretreatment of the samples, such as repeated extraction, evaporation and reconstitution procedures (17-21). The method proposed by Dockendorff et al .…”