Rapid and sensitive colorimetric detection of pathogens based on silver–urease interactions

Singh, Pargat; Kakkar, Saloni; Bharti, .; Kumar, Rajesh; Bhalla, Vijayender

doi:10.1039/c9cc00225a

Cited by 31 publications

(25 citation statements)

References 26 publications

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“…To overcome these limitations, diverse nanomaterials such as nanoparticles, nanorods, nanowires, and carbon nanostructures have been used because of their excellent physicochemical properties [12,13]. Noble metal nanoparticles such as those composed of gold and silver have been investigated based on their unique optical properties inducing a distinct color change by aggregation or chemical reaction on their surface [14,15].…”

Section: Introductionmentioning

confidence: 99%

A Convenient Colorimetric Bacteria Detection Method Utilizing Chitosan-Coated Magnetic Nanoparticles

Tran

Kim

2020

Nanomaterials

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An effective novel strategy to detect bacteria is promising because it may improve human health by allowing early diagnosis and timely treatment of bacterial infections. Here, we report a simple, reliable, and economical colorimetric assay using the peroxidase-like activity of chitosan-coated iron oxide magnetic nanoparticles (CS-MNPs). When CS-MNPs are incubated with a sample containing bacterial cells such as the gram-negative Escherichia coli or the gram-positive Staphylococcus aureus, the negatively-charged bacterial membrane interacts with positively-charged chitosan on the surface of CS-MNPs, thus resulting in significant reduction of their peroxidase-like activity presumably by a hindrance in the accessibility of the negatively charged substrate, 2-2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) to the positively-charged CS-MNPs. This simple colorimetric strategy allowed the rapid detection of bacterial cells down to 104 CFU mL−1 by the naked eye and 102 CFU mL−1 by spectrophotometry within 10 min. Based on the results, we anticipate that the CS-MNPs-based assay has great potential for the on-site diagnosis of bacterial infections in facility-limited or point-of-care testing (POCT) environments.

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Section: Introductionmentioning

confidence: 99%

A Convenient Colorimetric Bacteria Detection Method Utilizing Chitosan-Coated Magnetic Nanoparticles

Tran

Kim

2020

Nanomaterials

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“…Recently, a colorimetric sensor based on competitive binding of urease and bacterial cells toward antibody‐modified silver nanoparticles (AgNPs) was reported for S . Typhimurium detection with a sensitivity limit of 100 cells/mL (Singh, Kakkar, Bharti, Kumar, & Bhalla, 2019). Urease lost its catalytic activity upon adhering to AgNPs, whereas in the presence of the bacteria, AgNPs bound to the bacterial cells, rendering urease active in solution.…”

Section: Biosensors With Different Transduces For Detection Of Salmonmentioning

confidence: 99%

Biosensors for rapid detection of Salmonella in food: A review

Shen

2020

Comp Rev Food Sci Food Safe

146

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Salmonella is one of the main causes of foodborne infectious diseases, posing a serious threat to public health. It can enter the food supply chain at various stages of production, processing, distribution, and marketing. High prevalence of Salmonella necessitates efficient and effective approaches for its identification, detection, and monitoring at an early stage. Because conventional methods based on plate counting and real‐time polymerase chain reaction are time‐consuming and laborious, novel rapid detection methods are urgently needed for in‐field and on‐line applications. Biosensors provide many advantages over conventional laboratory assays in terms of sensitivity, specificity, and accuracy, and show superiority in rapid response and potential portability. They are now recognized as promising alternative tools and one of the most on‐site applicable and end user–accessible methods for rapid detection. In recent years, we have witnessed a flourishing of studies in the development of robust and elaborate biosensors for detection of Salmonella in food. This review aims to provide a comprehensive overview on Salmonella biosensors by highlighting different signal‐transducing mechanisms (optical, electrochemical, piezoelectric, etc.) and critically analyzing its recent trends, particularly in combination with nanomaterials, microfluidics, portable instruments, and smartphones. Furthermore, current challenges are emphasized and future perspectives are discussed.

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“…In order to develop a colorimetric assay which can be used by both UV-Vis spectrophotometry and a smartphone, the judicious choice of an appropriate chromogenic substrate that shows vivid color variations upon exposure to targets is vital. Fortunately, noble metal nanomaterials have been recognized as promising candidates owing to their high extinction coefficients and distinctive localized surface plasmon resonance (LSPR) properties [33][34][35][36][37][38]. Their LSPR absorption is highly dependent on their size, shape, and composition, leading to high color resolution [39,40].…”

Section: Introductionmentioning

confidence: 99%

Smartphone colorimetric assay of acid phosphatase based on a controlled iodine-mediated etching of gold nanorods

Liu

Huang

2020

Anal Bioanal Chem

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A simple but efficient colorimetric assay was developed for the detection and quantification of acid phosphatase (ACP) using a smartphone. This strategy is based on target-controlled iodine-mediated etching of gold nanorods (AuNRs). Due to effective hydrolysis of the substrate pyrophosphate (PPi) by ACP, chelated Cu 2+ with PPi was released, which promoted the redox reaction with an iodide ion (I −), leading to the formation of I 3 −. As the etching agent of AuNRs, I 3 − caused a blueshift of the localized surface plasmon resonance peak and, more importantly, an observable color change. The vivid colors were recorded with a smartphone camera and directly analyzed using an image-processing app. On the basis of the direct correlation between ACP concentration and the etching degree of AuNRs as well as color change, this smartphone nanocolorimetry technique showed a good linear response toward ACP over the range of 0-15.0 U/L, with a detection limit of 0.97 U/L. Using the standard addition method, the practical applicability of the proposed smartphone-based assay was successfully demonstrated by determining ACP in human serum samples, with results consistent with those obtained by UV-Vis spectrophotometry.

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Rapid and sensitive colorimetric detection of pathogens based on silver–urease interactions

Abstract: Herein, we demonstrate a facile and economic approach for colorimetric detection of microbial pathogens in drinking water, employing silver–urease interactions.

Cited by 31 publications

References 26 publications

A Convenient Colorimetric Bacteria Detection Method Utilizing Chitosan-Coated Magnetic Nanoparticles

A Convenient Colorimetric Bacteria Detection Method Utilizing Chitosan-Coated Magnetic Nanoparticles

Biosensors for rapid detection of Salmonella in food: A review

Smartphone colorimetric assay of acid phosphatase based on a controlled iodine-mediated etching of gold nanorods

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