Rapid and sensitive detection of multiple microRNAs in cell lysate by low-fouling surface plasmon resonance biosensor

Vaisocherová, Hana; Šípová, Hana; Víšová, Ivana; Bocková, Markéta; Špringer, Tomáš; Ermini, Maria Laura; Song, Xue; Krejčík, Zdeněk; Chrastinová, Leona; Pastva, Ondrej; Pimková, Kristýna; Merkerová, Michaela Dostálová; Dyr, Jan E.; Homola, Jiřı́

doi:10.1016/j.bios.2015.03.038

Cited by 85 publications

(46 citation statements)

References 51 publications

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“…In comparison with traditional detection methods, like X-ray, HPLC, and HPLC-MS, SPR technology can perform kinetics parameters (association and dissociation process) measurement, concentration measurement, and real-time molecule detection. It has been widely used in chemical, biology, agriculture, environment, and food safety [6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Zhang

Settu

Liu

2018

Advances in Condensed Matter Physics

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Surface plasmon resonance (SPR) is a real-time, label-free, and high-sensitive detection technology. SPR has been widely used in many applications such as biomolecular interaction analysis, environmental monitoring, and medical diagnostics. However, conventional SPR sensor systems usually require expensive equipment and complicated optics. In this paper, we have demonstrated a rapid prototyping of angle-scanning SPR for bioanalytical investigation. Rapid prototyping was attained by utilizing the FDM (fused deposition modeling) based 3D (three-dimensional) printing technology. Two rotating platforms were employed to drive the laser source and photodiode, respectively. A temperature regulation unit was incorporated to maintain the system temperature in order to reduce the temperature effect. The proposed SPR rapid prototyping yielded a refractive index resolution of 6.4×10 −6 RIU (refractive index unit), and the biotin-avidin system validated the kinetics parameters measurement capability. The obtained results indicated that the FDM 3D printing has great potential for developing rapid-prototyping SPR system.

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Section: Introductionmentioning

confidence: 99%

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Zhang

Settu

Liu

2018

Advances in Condensed Matter Physics

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“…It is clear that the reflected intensity pattern (IR) depends on several explicit and implicit parameters which are included in the components of the summation. While the explicit parameters are the film thickness (d), the refraction angle (ε1) and the refractive index of the film (the sensing layer) (n1), the implicit parameters are included in the reflection and transmission amplitudes (in conventional and polarized cases, Equations (4-6) and (8)(9)(10)(11), respectively), namely the refractive indices of the medium (n0), the film (n1) and the substrate (n2). The dependence of the reflected intensity on these parameters are demonstrated in Subsections 3.1-3.3, while two simulated, timeresolved adsorption experiments are presented in Subsections 3.4-3.5.…”

Section: Resultsmentioning

confidence: 99%

“…Several methods are known in the literature to detect the presence of any adsorbate: electric (resistive [1][2][3] and capacitive [4,5]) techniques operate mainly in the gas phase as volatile organic compound (VOC) sensors [6,7], while optical methods can be used both in gas and aqueous medium, mainly as biosensors. The latter group can be divided into plasmonic [8][9][10], waveguide [11,12] and reflectometric devices.…”

Section: Introductionmentioning

confidence: 99%

The Theoretical Concept of Polarization Reflectometric Interference Spectroscopy (PRIFS): An Optical Method to Monitor Molecule Adsorption and Nanoparticle Adhesion on the Surface of Thin Films

2019

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In this paper, we present an improved reflectometric interference spectroscopy (RIfS) sensor principle which is suitable for thin films. The conventional RIfS technique is an appropriate method to detect interfacial interactions at the solid–gas or solid–liquid interface in the case of thin films with a thickness of a few hundred nanometers, but when a significantly lower layer thickness (~100 nm) is required, the method is barely usable. By applying polarized reflected light and monitoring the ratio of the p- and s-polarized components, a characteristic curve can be obtained with one or a few local extreme value(s) with significantly favorable intensity ratios compared to the conventional method. In this work we studied the effect of film thickness, incident angle and the refractive indices of the thin film, the medium and the substrate. As a main result, it was demonstrated that the sensitivity of the PRIfS method is 4–7 times higher than that of the conventional technique near a critical angle. In simulated adsorption experiments, it was determined that the sensitivity of RIfS is around 550 nm/RIU (refractive index unit), while it is 1825 and 3966 nm/RIU for PRIfS in gas and aqueous phase, respectively.

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“…As high-sensitivity label free sensors, surface plasmon resonance (SPR) sensors (1)(2)(3) have been developed and used for various applications including medical diagnostics. Lately, as more compact optical biosensors with simpler structures, biosensors based on photonic devices have been proposed.…”

Section: Introductionmentioning

confidence: 99%

Improved Sensitivity of Microring Resonator-Loaded Mach–Zehnder Interferometer Biosensor

2017

Sensors and Materials

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We investigated the improvement in the sensitivity of a biosensor based on a silicon microring resonator-loaded Mach-Zehnder interferometer (MRR-MZI). Thermo-optic-driven variable optical attenuators (VOAs) were used to balance the optical power in one arm with that in the other to obtain a high extinction ratio of the MRR-MZI. The change in the transmittance as large as 12.9 and 7.5 dB for the detection of 200 and 20 pM avidin, respectively, was successfully obtained. These values are larger than those observed before the adjustment of the optical power balance by 4.8 and 5.3 dB, respectively. We discuss the characteristics of light power adjustment of the VOA and confirmation of biotin modification of the surface of the silicon waveguide using fluoresceinpolyethylene glycol-N-hydroxylsuccinimide (fluorescein-PEG-NHS).

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Rapid and sensitive detection of multiple microRNAs in cell lysate by low-fouling surface plasmon resonance biosensor

Cited by 85 publications

References 51 publications

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

The Theoretical Concept of Polarization Reflectometric Interference Spectroscopy (PRIFS): An Optical Method to Monitor Molecule Adsorption and Nanoparticle Adhesion on the Surface of Thin Films

Improved Sensitivity of Microring Resonator-Loaded Mach–Zehnder Interferometer Biosensor

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Rapid and sensitive detection of multiple microRNAs in cell lysate by low-fouling surface plasmon resonance biosensor

Cited by 85 publications

References 51 publications

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

The Theoretical Concept of Polarization Reflectometric Interference Spectroscopy (PRIFS): An Optical Method to Monitor Molecule Adsorption and Nanoparticle Adhesion on the Surface of Thin Films

Improved Sensitivity of Microring Resonator-Loaded Mach&ndash;Zehnder Interferometer Biosensor

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Improved Sensitivity of Microring Resonator-Loaded Mach–Zehnder Interferometer Biosensor