Rapid and sensitive detection of norovirus antibodies in human serum with a biolayer interferometry biosensor

Auer, Sanna; Koho, Tiia; Uusi-Kerttula, Hanni; Vesikari, Timo; Blazevic, Vesna; Hytönen, Vesa P.

doi:10.1016/j.snb.2015.06.088

Cited by 37 publications

(26 citation statements)

References 27 publications

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“…Aside from SERS and SPR, a number of other optical biosensor methods, including biolayer interferometry, fluorescence, bioluminescence and colorimetric based biosensors have also been shown to successfully detect NoV. For example, a label-and fluidic-free detection system based on a biolayer interferometry (BLI) biosensor has been proven to be able to detect NoV antibodies from human serum samples [145]. The scheme is depicted in Figure 3.…”

Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

“…The scheme is depicted in Figure 3. This assay is considered rapid (10~20 min if the sensor was pre-functionalized) and could detect NoV antibodies in serum dilutions up to 1:100,000 [145]. Similarly, Auer et al [144] used a Co (III)-NTA functionalized BLI sensor immobilized with His-tagged VLPs for the detection of NoV antibodies from human serum.…”

Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

“…Future research is needed to investigate the possible application of BLI based biosensors for direct HuNoV detection. [145] with permission from the publisher).…”

Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

“…The red and blue curve correspond to the NoV P-particle and VLP functionalized sensor, respectively. (Reprinted from Auer et al[145] with permission from the publisher).…”

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confidence: 99%

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A Survey of Analytical Techniques for Noroviruses

Liu

Moore

2020

Foods

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As the leading cause of acute gastroenteritis worldwide, human noroviruses (HuNoVs) have caused around 685 million cases of infection and nearly $60 billion in losses every year. Despite their highly contagious nature, an effective vaccine for HuNoVs has yet to become commercially available. Therefore, rapid detection and subtyping of noroviruses is crucial for preventing viral spread. Over the past half century, there has been monumental progress in the development of techniques for the detection and analysis of noroviruses. However, currently no rapid, portable assays are available to detect and subtype infectious HuNoVs. The purpose of this review is to survey and present different analytical techniques for the detection and characterization of noroviruses.

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Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

“…Future research is needed to investigate the possible application of BLI based biosensors for direct HuNoV detection. [145] with permission from the publisher).…”

Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

“…The red and blue curve correspond to the NoV P-particle and VLP functionalized sensor, respectively. (Reprinted from Auer et al[145] with permission from the publisher).…”

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confidence: 99%

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A Survey of Analytical Techniques for Noroviruses

Liu

Moore

2020

Foods

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“…For example, Selvarajah et al immobilized the chikungunya virus VLPs onto amine reactive biosensors to screen the response of two human IgG Fabs, C9 and E8. Auer et al used histidine‐tagged Noroviruses (Nov) VLPs on the Ni‐NTA sensors to directly detect its antibodies in human serum. Carvalho et al demonstrated the ability of BLI to detect and quantify label‐free influenza VLPs and model mucins.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Detection of the Interaction of Human Papillomavirus Virus‐Like Particles with Yeast Whole Cell RNA Using Biolayer Interferometry

Carta¹

2018

Biotechnology Journal

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RNA is a potential contaminant encountered in the production of recombinant yeast products such as proteins and virus-like particles (VLPs) and can be a particular concern when RNA associates with the product of interest. In this study a method to monitor and quantify the interaction of RNA with yeast-expressed human papillomavirus (HPV) VLPs using biolayer interferometry (BLI) is developed. Both biosensors with immobilized VLPs as well as biosensors with immobilized RNA are tested. Two types of recombinantly expressed HPV VLPs are used, Type 18 and Type 11. The data show that yeast whole cell RNA associates with Type 18 but not with Type 11 (maximum binding signal of 0.20 ± 0.02 nm on immobilized VLP probes for Type 18 vs. 0.03 ± 0.01 nm for Type 11). Additionally, the results show that a higher ionic strength weakens the affinity by both decreasing the association rate and increasing the dissociation rate, resulting in the dissociation constant increasing from 0.48 × 10 to 2.1 × 10 M, with phosphate ions having a greater effect on the reduction of association compared to chloride ions.

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Electrochemical approaches towards sensing viruses: A mini review

Santhanam

2020

Med Devices & Sens

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The world is exposed to a large number of pathogens that are primarily responsible for variety of diseases in humans and animals. These pathogens are divided into five broad categories such as viruses, bacteria, fungi, protozoa and worms. They enter the human body and utilize the body's resources to replicate, before exiting and infecting a new host, while weakening the immune system. In the class of pathogens, viruses are unique in that they kill host cells. We have been threatened by pathogens for centuries and our ability to reduce morbidity becomes obvious when we examine our average life span. Life expectancy of humans in the early 19th century was 40 years, which increased to 58 years in 1950 and then to 72 years in 1972. This gradual increase is due to better understanding of the biology of these pathogens, along with established methods for their detection and treatment. In 1940, influenza viruses A and B were detected and two vaccine protections against them were discovered in 1942. The H2N2 virus of 1957 resulted in deaths globally and led to the discovery of an antiviral medication in 1966. Another pandemic caused by H3N2 influenza virus resulted in several deaths in the United States and worldwide. The 2009 human influenza A H1N1 virus caused an annual global attack rate of 5%-10% in adults and 20%-30% in children; in one year (April 2009 to April 2010), 60.8 million cases were identified with a large number of deaths occurring in USA (https://www.cdc.gov/flu/pande mic-resou rces/2009-h1n1-pande mic.html, https://www.cdc.gov/flu/pande mic-resou rces/pande mic-timel ine-1930-and-beyond.html). Influenza virus infection occurred via inhalation of virus laden particles or direct contact with virus contaminated surfaces (Choi et al., 2002; Couch, 2003). The solution to the problem of virus attack is to detect them with precautions to suitably control or eliminate them. This review is oriented towards the detection of the viruses. 2 | ME THODOLOGY FOR DE TEC TION OF VIRUS E S Historically, respiratory virus infections have been diagnosed by a combination of techniques such as enzyme immune assay, direct fluorescence antibody, staining, cell culture and nucleic acid amplification

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Rapid and sensitive detection of norovirus antibodies in human serum with a biolayer interferometry biosensor

Cited by 37 publications

References 27 publications

A Survey of Analytical Techniques for Noroviruses

A Survey of Analytical Techniques for Noroviruses

Rapid and Sensitive Detection of the Interaction of Human Papillomavirus Virus‐Like Particles with Yeast Whole Cell RNA Using Biolayer Interferometry

Electrochemical approaches towards sensing viruses: A mini review

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