Rapid and sensitive ELISA method for the determination of bovine somatotropin in blood and milk

Zwickl, Craig; Smith, Holly W.; Bick, Peter H.

doi:10.1021/jf00096a013

Cited by 21 publications

(8 citation statements)

References 19 publications

(35 reference statements)

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“…The concentration of bST in various biological media was traditionally estimated using, either an enzyme-linked immunosorbent assay (ELISA) 5,6 a radioimmunoassay (RIA) 7,8 or bioassay methods. 9 The most important limitation of ELISA assays is that the concentration of antibody can only be reported in relative terms such as "titer," that is, a combination of affinity and concentration. More recently, a sensitive method that allows the discrimination between the recombinant and the endogenous forms of somatotropin has been developed.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoisland structures integrated in a lab-on-a-chip for plasmonic detection of bovine growth hormone

Ozhikandathil¹,

Bădilescu²,

Packirisamy³

2012

J. Biomed. Opt

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Three-dimensional gold nanostructures fabricated through a novel convective assembly method are treated thermally to obtain a nanoisland morphology. The new structure is proved to be adequate for the detection of bovine growth hormone, by using an immunoassay method based on the localized surface plasmon resonance band of gold. The nanoisland structures are integrated into a microfluidic device and the spectral measurements are carried out by introducing the device directly in the light beam of a ultraviolet-visible spectrophotometer. The principal motivation for this work is the need for a simple and rapid method of detection of hormone levels in milk and milk products.

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Section: Introductionmentioning

confidence: 99%

Gold nanoisland structures integrated in a lab-on-a-chip for plasmonic detection of bovine growth hormone

Ozhikandathil¹,

Bădilescu²,

Packirisamy³

2012

J. Biomed. Opt

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“…This strategy was successfully implemented by Zhao et al to determine the effects of rbGH administration on total bGH concentration in milk, concluding an observed increased concentration in treated animals by about 60% with usual treatment conditions [67]. Then, Zwickl et al developed an enzyme-linked immune-sorbent assay (ELISA) method that allowed, in four hours, the determination of total bGH in milk, with a LOD of 0.2 ng/mL [68]. In another study, bGH was successfully measured with an ECLIA (ElectroChemiLuminescence Immunoassay) allowing a LOD of 5 pg/mL [31].…”

Section: Direct Analytical Strategiesmentioning

confidence: 99%

The Promise and Challenges of Determining Recombinant Bovine Growth Hormone in Milk

Raux

Bichon

Benedetto

et al. 2022

Foods

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Recombinant bovine growth hormone (rbGH) is produced in large quantities and widely used in a number of countries worldwide to stimulate milk production in dairy animals. The use of this compound in animal production is strictly regulated by food safety directives in force, in particular in the European Union (EU). Although analytical strategies for the detection of rbGH in blood have been successfully reported over the past 15 years, they do not fully answer the expectations of either competent authorities or industrials that would expect measuring its occurrence directly in the milk. As a matrix of excretion but also of consumption, milk appears indeed as the matrix of choice for detecting the use of rbGH in dairy animals. It also allows large volumes to be collected without presenting an invasive character for the animal. However, rbGH detection in milk presents several challenges, mainly related to the sensitivity required for its detection in a complex biological matrix. This review article presents the specific difficulties associated with milk and provides an overview of the analytical strategies reported in the literature and whether they concern indirect or direct approaches to the detection of rbGH administration to animals, with applications either for screening or confirmation purposes.

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“…Des tentatives de dosages immunologiques des rGH dans le lait ou les tissus n'ont pas permis d'atteindre des limites de détection compatibles avec la recherche de ces composés dont les concentrations sont de l'ordre du ng/mL (Schams et al 1988, Fernandez et Kopchick 1990, Roh et al 1997. Certains dosages ELISA sur le plasma se sont avérés néanmoins prometteurs pour détecter la GH à des concentrations comprises entre 10 et 20 ng/mL dans cette matrice (Secchi et al 1988, Zwickl et al 1990, Roh et al 1997, Scippo et al 1997 mais n'ont jamais permis de distinguer la GH endogène de la forme recombinante.…”

Section: / Détection Directe De L'administration De Ghunclassified

Hormones et promoteurs de croissance en productions animales : de la physiologie à l’évaluation du risque

Paris¹,

André²,

Briant³

et al. 2006

INRA Prod. Anim.

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Au cours des cinquante dernières années, des progrès considérables dans les performances d’élevage des espèces domestiques ont été obtenus grâce à une meilleure connaissance acquise dans le domaine de la physiologie, en particulier en ce qui concerne la nutrition, la croissance, la reproduction et la lactation, et qui s’est conjuguée aux progrès génétiques accomplis dans les différentes espèces animales dans un contexte de meilleure maîtrise sanitaire des troupeaux et de maîtrise plus fine des systèmes d’élevage en place. Le recours aux substances hormonales pour mieux maîtriser les fonctions de reproduction et de croissance a pris une place déterminante dans ce progrès. Néanmoins, une tendance profonde consistant à restreindre leur utilisation à certains moments critiques de la vie de l’animal est apparue en Europe ces quinze dernières années, obligeant à rechercher des alternatives par le biais d’une meilleure maîtrise alimentaire, génétique et des systèmes d’élevage, ceci afin de garantir aux consommateurs une meilleure qualité définie a priori des produits animaux. Les fonctions physiologiques qui offrent des possibilités d’un contrôle hormonal exogène, les substances hormonales utilisables et les spéculations animales concernées sont présentées tour à tour. Un éclaircissement sur les risques toxicologiques que présentent ces substances, le contrôle et la traçabilité de leur utilisation et les réglementations en vigueur sont ensuite rappelés. Enfin, sont évoquées les recherches qui permettront de faire progresser sensiblement la question de l’utilisation des hormones en élevage dans un contexte sociétal renouvelé.

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Rapid and sensitive ELISA method for the determination of bovine somatotropin in blood and milk

Cited by 21 publications

References 19 publications

Gold nanoisland structures integrated in a lab-on-a-chip for plasmonic detection of bovine growth hormone

Gold nanoisland structures integrated in a lab-on-a-chip for plasmonic detection of bovine growth hormone

The Promise and Challenges of Determining Recombinant Bovine Growth Hormone in Milk

Hormones et promoteurs de croissance en productions animales : de la physiologie à l’évaluation du risque

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