Rapid and sensitive pollutant detection by induction of heat shock gene-bioluminescence gene fusions

Dyk, Tina K. Van; Majarian, William R.; Konstantinov, Konstantin; Young, Roslyn M.; Dhurjati, Prasad; LaRossa, Robert A.

doi:10.1128/aem.60.5.1414-1420.1994

Cited by 281 publications

(129 citation statements)

References 29 publications

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“…M strain with constant expression of degradation activity, and will be used to develop an approach to detect environmental contaminants. The utility of gene fusion consisting of a promoter of a TCE-speci¢c stress protein gene with the lux structural gene, as described by Dyk et al for heat-shock proteins [16], would be very useful to monitor environmental TCE contamination at sub-lethal concentrations.…”

Section: Resultsmentioning

confidence: 99%

Induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, Methylocystis sp. M

Uchiyama

1999

FEMS Microbiology Letters

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The responses of the trichloroethylene-degrading bacterium Methylocystis sp. M to six different water-pollutants, carbon starvation, and temperature-shock (heat and cold) were examined using 2-dimensional gel electrophoresis. Twenty-eight polypeptides were induced, and these stress-induced proteins were classified into three groups. Some of the chemically induced proteins were the same as those induced by carbon starvation and temperature-shock. Two of the polypeptides were induced by trichloroethylene. Trichloroethylene-stress protein synthesis required 1^2 h at a concentration of trichloroethylene that had no effect on growth. Furthermore, 25 stress-enhanced polypeptides were observed, and one of these was enhanced by trichloroethylene. Based on these results, we discuss applications of chemical-stress induction of proteins to establish effective bioremediation and bioassay by methanotrophs. z

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Section: Resultsmentioning

confidence: 99%

Induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, Methylocystis sp. M

Uchiyama

1999

FEMS Microbiology Letters

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“…In addition to the degradation rate and degree, the potential toxicity of the final decomposition products is also of crucial importance in estimating the efficiency of the enzymatic hydrolysis of EDCs. The recombinant BL E. coli used in this study has been extensively employed to estimate the toxic effects of various industrial wastes and pollutants (Nunoshiba and Nishioka 1991;Van Dyk et al 1994;Ptitsyn et al 1997;Min et al 1999;Davidov et al 2000). Although the detected toxicities are not directly related to the toxic effect in animals and/or humans, it is now generally accepted that they have potential for environmental monitoring and can also provide a reasonable basis for the primary screening of genotoxicants and stress-inducers (Van Dyk et al 1994;Ptitsyn et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Enhanced degradation and toxicity reduction of dihexyl phthalate by Fusarium oxysporum f. sp. pisi cutinase

Kim¹,

Seo²,

Min³

et al. 2007

J Appl Microbiol

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Aims: This research aims to investigate the efficiency of two lipolytic enzymes – fungal cutinase and yeast esterase – upon the biodegradation of dihexyl phthalate (DHP). Method and Results: During the enzymatic degradation of DHP dissolved in methanol, several degradation products were detected and their time‐course changes were monitored using GC/MS. The DHP‐degradation rate of cutinase was surprisingly high; i.e. almost 70% of the initial DHP (500 mg l−1) was decomposed within 4·5 h. Although the same amount of esterase was employed, more than 85% of the DHP remained after 3 days. Almost all the DHP was converted by cutinase into 1,3‐isobenzofurandione (IBF), whereas hexyl methyl phthalate and IBF were abundantly produced by esterase. In addition, the toxicities of the DHP‐degraded products by esterase were evaluated using various recombinant bioluminescent bacteria, which caused oxidative and protein damage, whereas the hydrolysis products from cutinase never caused any cellular damage in the methanol‐containing reaction system. Conclusions: Cutinase starts to act as a DHP‐degrader much earlier and faster than esterase, with high stability in ester‐hydrolytic activity, therefore a plausible approach to the practical application of cutinase for DHP degradation in the DHP‐contaminated environments may be possible. Significance and Impact of the Study: This study describes the enhanced degradation and detoxification of DHP using Fusarium oxysporum f. sp. pisi cutinase.

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“…The toxicity of a sample can also be evaluated with promoters that are induced by a broad spectrum of environmental insults and are thus good indicators of toxic cellular stress, such as the promoter of the grpE gene, a component of the chaperone network in E. coli (Van Dyk et al, 1994;de Marco et al, 2007). The use of two strains, one harbouring the plasmid recA′::GFPuv and the other grpE′::lux allowed an assessment of the toxicity of the sample along with its genotoxicity (Sagi et al, 2003).…”

Section: Cytotoxicity Controlsmentioning

confidence: 99%

Bacterial genotoxicity bioreporters

Biran

Yagur‐Kroll

Pedahzur

et al. 2010

Microbial Biotechnology

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SummaryEver since the introduction of the Salmonella typhimurium mammalian microsome mutagenicity assay (the ‘Ames test’) over three decades ago, there has been a constant development of additional genotoxicity assays based upon the use of genetically engineered microorganisms. Such assays rely either on reversion principles similar to those of the Ames test, or on promoter–reporter fusions that generate a quantifiable dose‐dependent signal in the presence of potential DNA damaging compounds and the induction of repair mechanisms; the latter group is the subject of the present review. Some of these assays were only briefly described in the scientific literature, whereas others have been developed all the way to commercial products. Out of these, only one, the umu‐test, has been fully validated and ISO‐ and OECD standardized. Here we review the main directions undertaken in the construction and testing of bacterial‐based genotoxicity bioassays, including the attempts to incorporate at least a partial metabolic activation capacity into the molecular design. We list the genetic modifications introduced into the tester strains, compare the performance of the different assays, and briefly describe the first attempts to incorporate such bacterial reporters into actual genotoxicity testing devices.

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Rapid and sensitive pollutant detection by induction of heat shock gene-bioluminescence gene fusions

Cited by 281 publications

References 29 publications

Induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, Methylocystis sp. M

Induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, Methylocystis sp. M

Enhanced degradation and toxicity reduction of dihexyl phthalate by Fusarium oxysporum f. sp. pisi cutinase

Bacterial genotoxicity bioreporters

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