“…Four general separation schemes have been devised for Cu based on the insolubility of its sulfide, the solvent extractability of its organic chelates, its cation exchange behavior and its electrodeposition properties. A rapid and simple method developed by Hahn et al (1968), consists of digesting the biological tissue in nitric acid, dissolving the residue in 4 ml of IN nitric acid, transferring the solution to a separatory funnel containing 2 ml of 64 O.IN HCl and extracting Cu with 5 ml of 0.01% dithizone in CCl. shaking for 2 minutes.…”