Rapid and simple screening analysis for residual imidacloprid in agricultural products with commercially available ELISA

Mammalian Detrimental Effects of Imidacloprid Residues in Tomato Fruits

“…Similar results was reported for imidacloprid, Fernandez-Alba et al (1996) reported that recovery percentage for imidacloprid were 123, 114 and 102% in pepper, tomato and cucumber fruits, respectively. Also, Watanabe et al (2004) found that the average rates of recovery for imidacloprid were 113.3, 88.0, 82.7 and 87.5% in cucumber, eggplant, lettuce and green pepper, respectively. Recovery percentages were used to correct data in residue studies for both insecticides.…”

Review on Current Analytical Methods with Chromatographic and Nonchromatographic Techniques for New Generation Insecticide Neonicotinoids

“…Table 3 shows that the easiest method to avoid the matrix effect is dilution of the sample extract (mainly methanol extract) with water or phosphate buffer. The recovery from various measurement samples were generally good in all reports (Byrne et al, 2005;Eisenback et al, 2009;Kim et al, 2006;Ma et al, 2009;Watanabe et al, 2001;Watanabe et al, 2004aWatanabe et al, , 2004bWatanabe et al, , 2006Watanabe et al, , 2011Xu et al, 2010). The measurement sensitivity of ELISA is apt to be affected by the concentration of extraction solvent (mainly methanol) coexisting in the sample solution during measurement (Nunes et al, 1998).…”

“…can be used easily for monitoring tests of a specific neonicotinoid insecticide. However, it is important in the use of kit-based ELISA to remember that the matrix effect should be evaluated in advance (Byrne et al, 2005;Watanabe et al, 2004aWatanabe et al, , 2004bWatanabe et al, , 2006Watanabe et al, , 2011. In any event, when ELISA is applied to a sample, it might be affected by the matrix effect.…”

The Present State and Perspective on Simple and Rapid Immunochemical Detection for Pesticide Residues in Crops

“…8, since all standard curves in diluted apple extracts substantially agreed with the control curves, the results suggest that any pesticides evaluated can be directly analyzed in the tested samples only by simple dilution of the extracts with water or water/methanol (9: 1) 29−32 . However, when the ELISA for imidacloprid was used to analyze spinach samples, avoiding matrix interference was difficult, even with additional dilution (data not shown) 30 . For such samples, a minimal sample pretreatment procedure such as SPE may be necessary to prevent matrix interference.…”

“…In contrast, the enzyme-linked immunosorbent assay (ELISA), which is based on highly specific or selective antigen-antibody interactions, gives a sensitive response against only one or a few trace level pesticides in various sample matrices, and is therefore a promising method for pesticide residue analysis 9,11,20,27 . Herein, I review the potential utility of ELISA for simple and rapid pesticide residue analysis in crops on the basis of previous research carried out in my laboratory to evaluate kit-based ELISAs for the neonicotinoid insecticide imidacloprid 29,30 , the organophosphorus insecticide fenitrothion 31 , and the fungicide chlorothalonil 32 ( Fig. 1) developed by Horiba Ltd. (Kyoto, Japan).…”