Rapid and simultaneous determination of hair polyamines as N-heptafluorobutyryl derivatives by gas chromatography–mass spectrometry

Li, Ling; Hara, Kenji; Liu, Junting; Yu, Yanping; Gao, Lina; Wang, Yan; Wang, Yanfang

doi:10.1016/j.jchromb.2008.10.054

Cited by 16 publications

(12 citation statements)

References 26 publications

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“…Each GC-MS derivatization and analytical method can only quantify a limited number of compounds with similar properties. Concurrent quantification of the three major polyamines together was challenging in relatively simpler biological samples such as hair (Choi et al , 2000; Li et al , 2008). The difficulty for quantification of spermine is mainly due to the presence of hiding active hydrogen atoms in its two secondary amine groups.…”

Section: Discussionmentioning

confidence: 99%

“…This is not a concern in mass spectrometry (MS), and when combined with gas chromatography, one of the most powerful analytical systems becomes available (GC-MS). In recent years, GC-MS has been used for polyamine analysis in simple biological samples such as hair (Li et al , 2008), urine (Paik et al , 2006) and plasma (Paik et al , 2007). However, a GC-MS method for analyses of the major polyamines from brain samples was not available.…”

Section: Introductionmentioning

confidence: 99%

“…We have recently developed a specific GC-MS method for measurement of spermidine and putrescine from post-mortem cerebral cortex (Chen et al , 2009). Although measurement of spermine by GC-MS has been shown from some biological samples such as hair and urine (Li et al , 2008; Paik et al , 2006), GC-MS quantification of spermine simultaneously with putrescine and spermidine is methodologically challenging in post-mortem brain tissues, and requires a separate methodology. Therefore, this study aimed at quantification of putrescine and spermidine in cerebral cortex derived from suicide completers and controls.…”

Section: Introductionmentioning

confidence: 99%

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Evidence of Altered Polyamine Concentrations in Cerebral Cortex of Suicide Completers

Chen

Fiori

Moquin

et al. 2010

Neuropsychopharmacol

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Recent studies have implicated alterations in the expression of polyamine-related genes in the brains of suicide completers including widespread downregulation of spermidine/spermine N1-acetyltransferase, the key enzyme in polyamine catabolism, suggesting compensatory mechanisms attempting to increase brain levels of polyamines. Given the complexity of the polyamine system, quantification of the levels of the polyamines is an essential step in understanding the downstream effects of dysregulated gene expression. We developed a method using high-resolution capillary gas chromatography (GC) in combination with mass spectrometry (MS) for quantitation of polyamines from post-mortem brain tissue, which allowed us to accurately measure spermidine and putrescine concentrations in post-mortem brain tissues. Using this method, we analyzed putrescine and spermidine levels in a total of 126 samples from Brodmann areas 4, 8/9, and 11, from 42 subjects, comprising 16 suicide completers with major depression, 13 non-depressed suicide completers, and 13 control subjects. Both putrescine and spermidine levels fell within the expected nanomolar ranges and were significantly elevated in the brain of suicide completers with a history of major depression as compared with controls. These results were not accounted by possible confounders. This is the first GC-MS study to analyze the expression of putrescine and spermidine from post-mortem brain tissue and confirms the hypothesis raised by previous studies indicating alterations in putrescine and spermidine levels in suicide/major depression.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evidence of Altered Polyamine Concentrations in Cerebral Cortex of Suicide Completers

Chen

Fiori

Moquin

et al. 2010

Neuropsychopharmacol

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“…Analytical methods for investigating polyamines and amino acids are mainly based on chromatographic separation using high-performance liquid chromatography (HPLC) or gas chromatography (GC) with pre-or post-column derivatization and then detection using traditional UV, fluorescence or mass spectrometry (MS) [13][14][15][16][17][18]. Fabio Gosetti et al had reported an HPLC-MS/MS method to determine sixteen biogenic amines, which included five polyamine metabolome [19].…”

Section: Introductionmentioning

confidence: 99%

Determination of polyamine metabolome in plasma and urine by ultrahigh performance liquid chromatography–tandem mass spectrometry method: Application to identify potential markers for human hepatic cancer

Liu

et al. 2013

Analytica Chimica Acta

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“…The gas chromatography-mass spectrometry (GC-MS) in selected ion-monitoring (SIM) method for simultaneous determination of putrescine, spermidine, and spermine as Nheptafluorobutyryl derivatives in hair samples was developed by Li et al They found that measuring hair polyamines may assist in early diagnosis and prevention of cancer in patients (79).…”

Section: Downloaded By [University Of Windsor] At 17:54 20 November 2014mentioning

confidence: 99%

Current Status of Polyamine and Polyamine Analogs Analysis in Cancer Research

Önal

2010

Critical Reviews in Analytical Chemistry

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The amino-acid-derived polyamines have been associated with cell growth and cancer and their concentrations in malignant tumors are extremely high compared to those in tissues that are histologically normal. Hence, polyamines have been considered as an interesting object for anticancer therapy. Analysis of polyamine levels in biological fluid can possibly provide helpful information on the types of cancer and progression phase of the disease. Numerous modern analytical methods, including high-performance liquid chromatography, gas chromatography, capillary electrophoresis, and other separation techniques have been widely utilized for analysing polyamine levels. In both tissue culture and experimental animal models, polyamine analogues restrain cell growth and destroy malignant cells. Determination of intracellular analogue contents is also crucial, since analogue accumulation is a causal factor for their antitumour effectiveness. In this review, the latest methods reported for polyamines and polyamine analogs in cancer research are discussed.

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Rapid and simultaneous determination of hair polyamines as N-heptafluorobutyryl derivatives by gas chromatography–mass spectrometry

Cited by 16 publications

References 26 publications

Evidence of Altered Polyamine Concentrations in Cerebral Cortex of Suicide Completers

Evidence of Altered Polyamine Concentrations in Cerebral Cortex of Suicide Completers

Determination of polyamine metabolome in plasma and urine by ultrahigh performance liquid chromatography–tandem mass spectrometry method: Application to identify potential markers for human hepatic cancer

Current Status of Polyamine and Polyamine Analogs Analysis in Cancer Research

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