Rapid and Transient Reduction in Circulating Thyroid Hormones Following Systemic Antigen Priming: Implications for Functional Collaboration between Dendritic Cells and Thyroid

An intrinsic thyrotropin-mediated pathway of TNF-α production by bone marrow cells

“…Previous studies from our laboratory reported murine TSH blood serum levels in the range of 20 to 40 ng/mL. 12 Although it is difficult to equate TSH produced in vitro with that of TSH produced under normal physiologic conditions in vivo, it is interesting that the concentration of TSH produced by CD11b ϩ BM cells in our assay was only slightly lower than that present in the circulation of normal mice. Collectively, these findings indicate that the CD11b ϩ cell population is the primary source of TSH in the murine BM.…”

“…23 As yet, we have not examined TSH production of BM cells after in vitro maturation, though we recently demonstrated that TSH is produced by purified splenic DCs. 12 However, the relationship of those cells to the BM cells described here has not been determined, though these studies are currently under way.…”

“…12 Briefly, 1.5 ϫ 10 6 cells were reacted for 10 minutes at 4°C with CD16/32 Fc receptor blocking reagent (BD Pharmingen). PE-or FITC-labeled anti-CD11b, anti-CD45, anti-TCR␤, anti-B220, or anti-Thy-1 mAb was added for 20 minutes at 4°C.…”

Characterization of a novel set of resident intrathyroidal bone marrow-derived hematopoietic cells: potential for immune-endocrine interactions in thyroid homeostasis

“…Of the studies done to date, phenotypic analyses have been limited or cells have been characterized using only CD11c as a marker of DCs (Quadbeck et al, 2002;Kabel et al, 1988;Bagriacik et al, 2001). However, DCs are an extremely heterogeneous population of cells, consisting of phenotypic subsets that can be differentiated in part according to lineage variations, distribution within secondary lymphoid tissues, and changes that occur as cells proceed from an immature to mature or activated form Pulendran et al, 1997;Vrmec and Shortman, 1997;Ardavin et al, 1993;Kronin et al, 1997;Saunders et al, 1996;Wu et al, 1995).…”

Dendritic cells in autoimmune disorders and cancer of the thyroid

“…This suggestion seems to be further supported by the fact that murine DCs located in secondary lymphoid tissues were shown to produce and secrete TSH and in vitro studies confirmed DCs as the most potent known TSH producers in immune system. Interestingly, TSH production by DCs increased considerably in response to bacterial products [115,116], suggesting possible role of DC associated auto-and paracrine TSH secretion in primary immune response.…”