Rapid antimicrobial susceptibility testing for low bacterial concentrations integrating a centrifuge based bacterial cell concentrator

Abstract

Antibiotic resistance threatens human health worldwide. Patients infected with antibiotic-resistant bacteria require appropriate antibiotic prescriptions based on a rapid antibiotic susceptibility test (AST). Various rapid AST methods have been developed...

A capillary-based centrifugal indicator equipped with in situ pathogenic bacteria culture for fast antimicrobial susceptibility testing

“…To demonstrate the difference in the inhibition of bacterial growth more clearly, a threshold where the column height was less than 50% of the control group was given here for MIC determination. 34–36…”

“…To demonstrate the difference in the inhibition of bacterial growth more clearly, a threshold where the column height was less than 50% of the control group was given here for MIC determination. [34][35][36] For the ATCC 35218 strains, there was no obvious decrease in the column height compared to the control group in 1-100 μg mL −1 ampicillin after 3 h incubation (Fig. 6c).…”

Integrated Microfluidic Chip for Rapid Antimicrobial Susceptibility Testing Directly from Positive Blood Cultures

“…Recently, microfluidic methods have been utilized for bacterial analysis owing to their speed, high throughput, and small footprint. Various microfluidic platforms were provided for rapid AST and minimum inhibitory concentration (MIC) determination. – These devices focus on reducing the assay time or increasing the assay throughput by utilizing high aspect ratio channels, droplets, ,, dielectrophoresis, , concentration gradient generators, , and centrifugal microfluidics. ,, Compared with traditional bulk incubation, the bacteria growing on-chip can reach their MIC value much faster, allowing antimicrobial resistance analysis to be completed in as little as 2 h and reducing reagent and sample consumption . By increasing the number of chambers, these devices can also provide the opportunity to analyze multiple antibiotics and multiple samples in parallel.…”

“…Unlike other microfluidic devices, centrifugal microfluidic technology does not require complex instruments, and it allows for the rapid acquisition of sufficient microbial cells for downstream AST and identification, avoiding the lengthy incubation process of traditional methods. ,, For example, Cho et al developed a custom-made fidget spinner that could rapidly concentrate pathogens in 1 mL of undiluted urine by more than 100-fold for the on-device colorimetric detection of bacterial load and pathogen identification, which can be used for AST . Xu et al presented a centrifugation-based microfluidic concentrator for milliliter to picoliter bacteria suspension enrichment to achieve fast and real-time pathogen detection and AST within 3 h .…”